Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish
Abstract Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects a...
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2021
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oai:doaj.org-article:13c3e7c8a66640889a25179ca2872f082021-12-02T14:04:39ZAltered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish10.1038/s41598-021-83345-32045-2322https://doaj.org/article/13c3e7c8a66640889a25179ca2872f082021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83345-3https://doaj.org/toc/2045-2322Abstract Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects are accompanied with changes in the expression profile of non-coding RNAs, inherited by next generations. Non-coding RNA expression profile was analysed in F1 offspring (5.5 h post-fertilization) by high-throughput sequencing 1 year after parental irradiation (8.7 mGy/h, 5.2 Gy total dose). Using our previous F1-γ genome-wide gene expression data (GSE98539), hundreds of mRNAs were predicted as targets of differentially expressed (DE) miRNAs, involved in pathways such as insulin receptor, NFkB and PTEN signalling, linking to apoptosis and cancer. snRNAs belonging to the five major spliceosomal snRNAs were down-regulated in the F1-γ group, Indicating transcriptional and post-transcriptional alterations. In addition, DEpiRNA clusters were associated to 9 transposable elements (TEs) (LTR, LINE, and TIR) (p = 0.0024), probable as a response to the activation of these TEs. Moreover, the expression of the lincRNAs malat-1, and several others was altered in the offspring F1, in concordance with previously observed phenotypical alterations. In conclusion, our results demonstrate diverse gamma radiation-induced alterations in the ncRNA profiles of F1 offspring observable 1 year after parental irradiation.Leonardo MartínJorke H. KamstraSelma HuremLeif C. LindemanDag A. BredeHåvard AanesIgor BabiakAmilcar ArenalDeborah OughtonBrit SalbuJan Ludvig LychePeter AleströmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Leonardo Martín Jorke H. Kamstra Selma Hurem Leif C. Lindeman Dag A. Brede Håvard Aanes Igor Babiak Amilcar Arenal Deborah Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| description |
Abstract Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects are accompanied with changes in the expression profile of non-coding RNAs, inherited by next generations. Non-coding RNA expression profile was analysed in F1 offspring (5.5 h post-fertilization) by high-throughput sequencing 1 year after parental irradiation (8.7 mGy/h, 5.2 Gy total dose). Using our previous F1-γ genome-wide gene expression data (GSE98539), hundreds of mRNAs were predicted as targets of differentially expressed (DE) miRNAs, involved in pathways such as insulin receptor, NFkB and PTEN signalling, linking to apoptosis and cancer. snRNAs belonging to the five major spliceosomal snRNAs were down-regulated in the F1-γ group, Indicating transcriptional and post-transcriptional alterations. In addition, DEpiRNA clusters were associated to 9 transposable elements (TEs) (LTR, LINE, and TIR) (p = 0.0024), probable as a response to the activation of these TEs. Moreover, the expression of the lincRNAs malat-1, and several others was altered in the offspring F1, in concordance with previously observed phenotypical alterations. In conclusion, our results demonstrate diverse gamma radiation-induced alterations in the ncRNA profiles of F1 offspring observable 1 year after parental irradiation. |
| format |
article |
| author |
Leonardo Martín Jorke H. Kamstra Selma Hurem Leif C. Lindeman Dag A. Brede Håvard Aanes Igor Babiak Amilcar Arenal Deborah Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström |
| author_facet |
Leonardo Martín Jorke H. Kamstra Selma Hurem Leif C. Lindeman Dag A. Brede Håvard Aanes Igor Babiak Amilcar Arenal Deborah Oughton Brit Salbu Jan Ludvig Lyche Peter Aleström |
| author_sort |
Leonardo Martín |
| title |
Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| title_short |
Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| title_full |
Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| title_fullStr |
Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| title_full_unstemmed |
Altered non-coding RNA expression profile in F1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| title_sort |
altered non-coding rna expression profile in f1 progeny 1 year after parental irradiation is linked to adverse effects in zebrafish |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/13c3e7c8a66640889a25179ca2872f08 |
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