THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION

The possibility of post-vaccination anti-plague immunity evaluation was researched using antigen-stimulated cells tests in vitro and cytometry analysis. The object of study — the blood samples of 17 people immunised by the live plague vaccine (Yersinia pestis EV) epicutaneously. Blood taking was car...

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Autores principales:	A. N. Kulichenko, N. V. Abzaeva, S. E. Gostischeva, E. L. Rakitina, D. G. Ponomarenko, M. V. Kostuchenko
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	specific plague prevention cellular immunity evaluation of the effectiveness of vaccination lymphocyte activation markers yersinia pestis antigens stimulating coefficient Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/13c3f2c3010a48daa3f57ea9bbbc7170
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spelling	oai:doaj.org-article:13c3f2c3010a48daa3f57ea9bbbc71702021-11-22T07:09:48ZTHE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION2220-76192313-739810.15789/2220-7619-2017-2-203-208https://doaj.org/article/13c3f2c3010a48daa3f57ea9bbbc71702017-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/520https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The possibility of post-vaccination anti-plague immunity evaluation was researched using antigen-stimulated cells tests in vitro and cytometry analysis. The object of study — the blood samples of 17 people immunised by the live plague vaccine (Yersinia pestis EV) epicutaneously. Blood taking was carried out before vaccination and after immunisation on 7 and on 21 days, in 3 and in 6 months. Intensity antigen reactivity of lymphocytes was detected by cell tests in vitro, analysing markers of early (CD45+CD3+CD25+) and late (CD45+CD3+HLA-DR+) lymphocyte activation using flow cytometry. The complex of water-soluble Y. pestis antigens and allergen — pestin PP was tested as antigen. The high stimulating potential was defined of the water-soluble antigens Y. pestis complex. It is shown that coefficient of stimulation of relative level T- lymphocytes which express receptors for IL-2 was positive for all observation times after immunisation. The coefficient of stimulation had maximum values at 21 days (56.37%) and at 3 (47.41%) months. In identifying HLADR-positive lymphocytes before vaccination, the negative coefficient of stimulation was indicated on 7 and 21 days and the positive coefficient of stimulation was indicated at 3 and at 6 months. Analysis of intensity expression of early and late lymphocyte activation markers dynamics showed the possibility and prospect of application of cellular in vitro tests for the laboratory evaluation of specific reactivity of cellular immunity in both the early (7 days) and late (6 months) periods after vaccination. The results can be the basis for developing a new algorithm for assessment of immunological effectiveness of vaccination people against plague. It is the algorithm based on the identification of lymphocyte activation markers by antigen stimulation in conditions in vitro.A. N. KulichenkoN. V. AbzaevaS. E. GostischevaE. L. RakitinaD. G. PonomarenkoM. V. KostuchenkoSankt-Peterburg : NIIÈM imeni Pasteraarticlespecific plague preventioncellular immunityevaluation of the effectiveness of vaccinationlymphocyte activation markersyersinia pestis antigensstimulating coefficientInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 2, Pp 203-208 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	specific plague prevention cellular immunity evaluation of the effectiveness of vaccination lymphocyte activation markers yersinia pestis antigens stimulating coefficient Infectious and parasitic diseases RC109-216
spellingShingle	specific plague prevention cellular immunity evaluation of the effectiveness of vaccination lymphocyte activation markers yersinia pestis antigens stimulating coefficient Infectious and parasitic diseases RC109-216 A. N. Kulichenko N. V. Abzaeva S. E. Gostischeva E. L. Rakitina D. G. Ponomarenko M. V. Kostuchenko THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
description	The possibility of post-vaccination anti-plague immunity evaluation was researched using antigen-stimulated cells tests in vitro and cytometry analysis. The object of study — the blood samples of 17 people immunised by the live plague vaccine (Yersinia pestis EV) epicutaneously. Blood taking was carried out before vaccination and after immunisation on 7 and on 21 days, in 3 and in 6 months. Intensity antigen reactivity of lymphocytes was detected by cell tests in vitro, analysing markers of early (CD45+CD3+CD25+) and late (CD45+CD3+HLA-DR+) lymphocyte activation using flow cytometry. The complex of water-soluble Y. pestis antigens and allergen — pestin PP was tested as antigen. The high stimulating potential was defined of the water-soluble antigens Y. pestis complex. It is shown that coefficient of stimulation of relative level T- lymphocytes which express receptors for IL-2 was positive for all observation times after immunisation. The coefficient of stimulation had maximum values at 21 days (56.37%) and at 3 (47.41%) months. In identifying HLADR-positive lymphocytes before vaccination, the negative coefficient of stimulation was indicated on 7 and 21 days and the positive coefficient of stimulation was indicated at 3 and at 6 months. Analysis of intensity expression of early and late lymphocyte activation markers dynamics showed the possibility and prospect of application of cellular in vitro tests for the laboratory evaluation of specific reactivity of cellular immunity in both the early (7 days) and late (6 months) periods after vaccination. The results can be the basis for developing a new algorithm for assessment of immunological effectiveness of vaccination people against plague. It is the algorithm based on the identification of lymphocyte activation markers by antigen stimulation in conditions in vitro.
format	article
author	A. N. Kulichenko N. V. Abzaeva S. E. Gostischeva E. L. Rakitina D. G. Ponomarenko M. V. Kostuchenko
author_facet	A. N. Kulichenko N. V. Abzaeva S. E. Gostischeva E. L. Rakitina D. G. Ponomarenko M. V. Kostuchenko
author_sort	A. N. Kulichenko
title	THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
title_short	THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
title_full	THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
title_fullStr	THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
title_full_unstemmed	THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION
title_sort	antigen-specific cell in vitro tests for post-vaccination antiplague immunity formation
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/13c3f2c3010a48daa3f57ea9bbbc7170
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THE ANTIGEN-SPECIFIC CELL IN VITRO TESTS FOR POST-VACCINATION ANTIPLAGUE IMMUNITY FORMATION

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