Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps

Cancer-associated thrombosis is the second-leading cause of mortality in patients with cancer and presents a poor prognosis, with a lack of effective treatment strategies. NAD(P)H quinone oxidoreductase 1 (NQO1) increases the cellular nicotinamide adenine dinucleotide (NAD<sup>+</sup>) l...

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Autores principales: Wa Cao, Meng-Yu Zhu, Seung-Hoon Lee, Su-Bin Lee, Hyung-Jin Kim, Byung-Ouk Park, Cheol-Hwan Yoon, Dipendra Khadka, Gi-Su Oh, Hyeok Shim, Tae-Hwan Kwak, Hong-Seob So
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spelling oai:doaj.org-article:13c7afa97e9841b28f1e57e3c3b7e4822021-11-11T17:28:31ZModulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps10.3390/ijms2221120851422-00671661-6596https://doaj.org/article/13c7afa97e9841b28f1e57e3c3b7e4822021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12085https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cancer-associated thrombosis is the second-leading cause of mortality in patients with cancer and presents a poor prognosis, with a lack of effective treatment strategies. NAD(P)H quinone oxidoreductase 1 (NQO1) increases the cellular nicotinamide adenine dinucleotide (NAD<sup>+</sup>) levels by accelerating the oxidation of NADH to NAD<sup>+</sup>, thus playing important roles in cellular homeostasis, energy metabolism, and inflammatory responses. Using a murine orthotopic 4T1 breast cancer model, in which multiple thrombi are generated in the lungs at the late stage of cancer development, we investigated the effects of regulating the cellular NAD<sup>+</sup> levels on cancer-associated thrombosis. In this study, we show that dunnione (a strong substrate of NQO1) attenuates the prothrombotic state and lung thrombosis in tumor-bearing mice by inhibiting the expression of tissue factor and formation of neutrophil extracellular traps (NETs). Dunnione increases the cellular NAD<sup>+</sup> levels in lung tissues of tumor-bearing mice to restore the declining sirtuin 1 (SIRT1) activity, thus deacetylating nuclear factor-kappa B (NF-κB) and preventing the overexpression of tissue factor in bronchial epithelial and vascular endothelial cells. In addition, we demonstrated that dunnione abolishes the ability of neutrophils to generate NETs by suppressing histone acetylation and NADPH oxidase (NOX) activity. Overall, our results reveal that the regulation of cellular NAD<sup>+</sup> levels by pharmacological agents may inhibit pulmonary embolism in tumor-bearing mice, which may potentially be used as a viable therapeutic approach for the treatment of cancer-associated thrombosis.Wa CaoMeng-Yu ZhuSeung-Hoon LeeSu-Bin LeeHyung-Jin KimByung-Ouk ParkCheol-Hwan YoonDipendra KhadkaGi-Su OhHyeok ShimTae-Hwan KwakHong-Seob SoMDPI AGarticlecancer-associated thrombosisNAD<sup>+</sup>dunnionetissue factorNETsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12085, p 12085 (2021)
institution DOAJ
collection DOAJ
language EN
topic cancer-associated thrombosis
NAD<sup>+</sup>
dunnione
tissue factor
NETs
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle cancer-associated thrombosis
NAD<sup>+</sup>
dunnione
tissue factor
NETs
Biology (General)
QH301-705.5
Chemistry
QD1-999
Wa Cao
Meng-Yu Zhu
Seung-Hoon Lee
Su-Bin Lee
Hyung-Jin Kim
Byung-Ouk Park
Cheol-Hwan Yoon
Dipendra Khadka
Gi-Su Oh
Hyeok Shim
Tae-Hwan Kwak
Hong-Seob So
Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
description Cancer-associated thrombosis is the second-leading cause of mortality in patients with cancer and presents a poor prognosis, with a lack of effective treatment strategies. NAD(P)H quinone oxidoreductase 1 (NQO1) increases the cellular nicotinamide adenine dinucleotide (NAD<sup>+</sup>) levels by accelerating the oxidation of NADH to NAD<sup>+</sup>, thus playing important roles in cellular homeostasis, energy metabolism, and inflammatory responses. Using a murine orthotopic 4T1 breast cancer model, in which multiple thrombi are generated in the lungs at the late stage of cancer development, we investigated the effects of regulating the cellular NAD<sup>+</sup> levels on cancer-associated thrombosis. In this study, we show that dunnione (a strong substrate of NQO1) attenuates the prothrombotic state and lung thrombosis in tumor-bearing mice by inhibiting the expression of tissue factor and formation of neutrophil extracellular traps (NETs). Dunnione increases the cellular NAD<sup>+</sup> levels in lung tissues of tumor-bearing mice to restore the declining sirtuin 1 (SIRT1) activity, thus deacetylating nuclear factor-kappa B (NF-κB) and preventing the overexpression of tissue factor in bronchial epithelial and vascular endothelial cells. In addition, we demonstrated that dunnione abolishes the ability of neutrophils to generate NETs by suppressing histone acetylation and NADPH oxidase (NOX) activity. Overall, our results reveal that the regulation of cellular NAD<sup>+</sup> levels by pharmacological agents may inhibit pulmonary embolism in tumor-bearing mice, which may potentially be used as a viable therapeutic approach for the treatment of cancer-associated thrombosis.
format article
author Wa Cao
Meng-Yu Zhu
Seung-Hoon Lee
Su-Bin Lee
Hyung-Jin Kim
Byung-Ouk Park
Cheol-Hwan Yoon
Dipendra Khadka
Gi-Su Oh
Hyeok Shim
Tae-Hwan Kwak
Hong-Seob So
author_facet Wa Cao
Meng-Yu Zhu
Seung-Hoon Lee
Su-Bin Lee
Hyung-Jin Kim
Byung-Ouk Park
Cheol-Hwan Yoon
Dipendra Khadka
Gi-Su Oh
Hyeok Shim
Tae-Hwan Kwak
Hong-Seob So
author_sort Wa Cao
title Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
title_short Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
title_full Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
title_fullStr Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
title_full_unstemmed Modulation of Cellular NAD<sup>+</sup> Attenuates Cancer-Associated Hypercoagulability and Thrombosis via the Inhibition of Tissue Factor and Formation of Neutrophil Extracellular Traps
title_sort modulation of cellular nad<sup>+</sup> attenuates cancer-associated hypercoagulability and thrombosis via the inhibition of tissue factor and formation of neutrophil extracellular traps
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/13c7afa97e9841b28f1e57e3c3b7e482
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