Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential

Abstract Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus...

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Autores principales:	Johanna Muurinen, Jacob Richert, Carmen L. Wickware, Brian Richert, Timothy A. Johnson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13c9a48334fa48568e015b98b7167958
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spelling	oai:doaj.org-article:13c9a48334fa48568e015b98b71679582021-12-02T11:35:52ZSwine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential10.1038/s41598-021-84759-92045-2322https://doaj.org/article/13c9a48334fa48568e015b98b71679582021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84759-9https://doaj.org/toc/2045-2322Abstract Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more information is needed on their capability to disseminate antimicrobial resistance compared to antibiotics. We investigated the impacts of carbadox (antibiotic), copper sulfate and zinc oxide (metals) and mushroom powder (natural product) on the pig fecal resistome and microbiome. Antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances were measured using a high-throughput qPCR array with 382 primer pairs. Bacterial community composition was determined by 16S rRNA gene sequencing. More ARGs co-occurred with MGEs in the growth promoter group samples than in the control group samples. Community composition could not be linked to resistome in the growth promoter group samples, indicating a potential decoupling of ARGs and phylogeny. Additionally, machine-learning methods aided in defining the community and resistome differences in response to treatments. Since increased ARG mobility potential was the primary response to the dietary additives used in this study, we suggest that ARG mobility should be considered when designing antimicrobial use policies and antimicrobial resistance surveillances.Johanna MuurinenJacob RichertCarmen L. WickwareBrian RichertTimothy A. JohnsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johanna Muurinen Jacob Richert Carmen L. Wickware Brian Richert Timothy A. Johnson Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
description	Abstract Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more information is needed on their capability to disseminate antimicrobial resistance compared to antibiotics. We investigated the impacts of carbadox (antibiotic), copper sulfate and zinc oxide (metals) and mushroom powder (natural product) on the pig fecal resistome and microbiome. Antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances were measured using a high-throughput qPCR array with 382 primer pairs. Bacterial community composition was determined by 16S rRNA gene sequencing. More ARGs co-occurred with MGEs in the growth promoter group samples than in the control group samples. Community composition could not be linked to resistome in the growth promoter group samples, indicating a potential decoupling of ARGs and phylogeny. Additionally, machine-learning methods aided in defining the community and resistome differences in response to treatments. Since increased ARG mobility potential was the primary response to the dietary additives used in this study, we suggest that ARG mobility should be considered when designing antimicrobial use policies and antimicrobial resistance surveillances.
format	article
author	Johanna Muurinen Jacob Richert Carmen L. Wickware Brian Richert Timothy A. Johnson
author_facet	Johanna Muurinen Jacob Richert Carmen L. Wickware Brian Richert Timothy A. Johnson
author_sort	Johanna Muurinen
title	Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_short	Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_full	Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_fullStr	Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_full_unstemmed	Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_sort	swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/13c9a48334fa48568e015b98b7167958
work_keys_str_mv	AT johannamuurinen swinegrowthpromotionwithantibioticsoralternativescanincreaseantibioticresistancegenemobilitypotential AT jacobrichert swinegrowthpromotionwithantibioticsoralternativescanincreaseantibioticresistancegenemobilitypotential AT carmenlwickware swinegrowthpromotionwithantibioticsoralternativescanincreaseantibioticresistancegenemobilitypotential AT brianrichert swinegrowthpromotionwithantibioticsoralternativescanincreaseantibioticresistancegenemobilitypotential AT timothyajohnson swinegrowthpromotionwithantibioticsoralternativescanincreaseantibioticresistancegenemobilitypotential
_version_	1718395804921626624

Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential

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