miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer

MicroRNAs (miRNAs) operate as tumor suppressor or carcinogen to regulate cell proliferation, metastasis, invasion, differentiation, apoptosis, and metabolic process. In the present research, we investigated the effect and mechanism of miR-496 in human gastric cancer cells. miR-496 was downregulated...

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Autores principales:	Su Rui, Zhao Enhong, Zhang Jun
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	akt/mtor signaling pathway apoptosis binding site mir-496 3ʹ-utr Medicine R
Acceso en línea:	https://doaj.org/article/13d2117e26a7491ebabe234314500045
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spelling	oai:doaj.org-article:13d2117e26a7491ebabe2343145000452021-12-05T14:10:54ZmiR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer2391-546310.1515/med-2021-0313https://doaj.org/article/13d2117e26a7491ebabe2343145000452021-08-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0313https://doaj.org/toc/2391-5463MicroRNAs (miRNAs) operate as tumor suppressor or carcinogen to regulate cell proliferation, metastasis, invasion, differentiation, apoptosis, and metabolic process. In the present research, we investigated the effect and mechanism of miR-496 in human gastric cancer cells. miR-496 was downregulated in two gastric cancer cell lines, AGS and MKN45, compared with normal gastric epithelial cell line GES-1. miR-496 mimics inhibited the proliferation of AGS cells after the transfection for 48 and 72 h. The migration and invasion of AGS cells were also inhibited by the transfection of miR-496 mimics. miR-496 mimics induced the apoptosis through upregulating the levels of Bax and Active Caspase 3 and downregulating the levels of Bcl-2 and Total Caspase 3. Bioinformatics analysis showed that there was a binding site between miR-496 and Lyn kinase (LYN). miR-496 mimics could inhibit the expression of LYN in AGS cells. LYN overexpression blocked the inhibition of tumor cell growth, as well as the inhibition of AKT/mTOR signaling pathway induced by miR-496. In conclusion, miR-496 inhibited the proliferation through the AKT/mTOR signaling pathway via targeting LYN in gastric cancer cells. Our research provides a new potential target for clinical diagnosis and targeted treatment for gastric cancer.Su RuiZhao EnhongZhang JunDe Gruyterarticleakt/mtor signaling pathwayapoptosisbinding sitemir-4963ʹ-utrMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1206-1214 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	akt/mtor signaling pathway apoptosis binding site mir-496 3ʹ-utr Medicine R
spellingShingle	akt/mtor signaling pathway apoptosis binding site mir-496 3ʹ-utr Medicine R Su Rui Zhao Enhong Zhang Jun miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
description	MicroRNAs (miRNAs) operate as tumor suppressor or carcinogen to regulate cell proliferation, metastasis, invasion, differentiation, apoptosis, and metabolic process. In the present research, we investigated the effect and mechanism of miR-496 in human gastric cancer cells. miR-496 was downregulated in two gastric cancer cell lines, AGS and MKN45, compared with normal gastric epithelial cell line GES-1. miR-496 mimics inhibited the proliferation of AGS cells after the transfection for 48 and 72 h. The migration and invasion of AGS cells were also inhibited by the transfection of miR-496 mimics. miR-496 mimics induced the apoptosis through upregulating the levels of Bax and Active Caspase 3 and downregulating the levels of Bcl-2 and Total Caspase 3. Bioinformatics analysis showed that there was a binding site between miR-496 and Lyn kinase (LYN). miR-496 mimics could inhibit the expression of LYN in AGS cells. LYN overexpression blocked the inhibition of tumor cell growth, as well as the inhibition of AKT/mTOR signaling pathway induced by miR-496. In conclusion, miR-496 inhibited the proliferation through the AKT/mTOR signaling pathway via targeting LYN in gastric cancer cells. Our research provides a new potential target for clinical diagnosis and targeted treatment for gastric cancer.
format	article
author	Su Rui Zhao Enhong Zhang Jun
author_facet	Su Rui Zhao Enhong Zhang Jun
author_sort	Su Rui
title	miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
title_short	miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
title_full	miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
title_fullStr	miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
title_full_unstemmed	miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer
title_sort	mir-496 inhibits proliferation via lyn and akt pathway in gastric cancer
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/13d2117e26a7491ebabe234314500045
work_keys_str_mv	AT surui mir496inhibitsproliferationvialynandaktpathwayingastriccancer AT zhaoenhong mir496inhibitsproliferationvialynandaktpathwayingastriccancer AT zhangjun mir496inhibitsproliferationvialynandaktpathwayingastriccancer
_version_	1718371584832438272

miR-496 inhibits proliferation via LYN and AKT pathway in gastric cancer

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