Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning
Abstract T-cell-redirecting bispecific antibodies have emerged as a new class of therapeutic agents designed to simultaneously bind to T cells via CD3 and to tumor cells via tumor-cell-specific antigens (TSA), inducing T-cell-mediated killing of tumor cells. The promising preclinical and clinical ef...
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Nature Portfolio
2021
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oai:doaj.org-article:13db8e3e17e043ffbbadbbb6bb988d632021-12-02T16:14:03ZGeneration of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning10.1038/s41598-021-93842-02045-2322https://doaj.org/article/13db8e3e17e043ffbbadbbb6bb988d632021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93842-0https://doaj.org/toc/2045-2322Abstract T-cell-redirecting bispecific antibodies have emerged as a new class of therapeutic agents designed to simultaneously bind to T cells via CD3 and to tumor cells via tumor-cell-specific antigens (TSA), inducing T-cell-mediated killing of tumor cells. The promising preclinical and clinical efficacy of TSAxCD3 antibodies is often accompanied by toxicities such as cytokine release syndrome due to T-cell activation. How the efficacy and toxicity profile of the TSAxCD3 bispecific antibodies depends on the binding affinity to CD3 remains unclear. Here, we evaluate bispecific antibodies that were engineered to have a range of CD3 affinities, while retaining the same binding affinity for the selected tumor antigen. These agents were tested for their ability to kill tumor cells in vitro, and their biodistribution, serum half-life, and anti-tumor activity in vivo. Remarkably, by altering the binding affinity for CD3 alone, we can generate bispecific antibodies that maintain potent killing of TSA + tumor cells but display differential patterns of cytokine release, pharmacokinetics, and biodistribution. Therefore, tuning CD3 affinity is a promising method to improve the therapeutic index of T-cell-engaging bispecific antibodies.Lauric HaberKara OlsonMarcus P. KellyAlison CrawfordDavid J. DiLilloRichard TavaréErica UllmanShu MaoLauren CanovaOlga SineshchekovaJennifer FinneyArpita PawasheSupriya PatelRyan McKaySahar RizviErmelinda DamkoDanica ChiuKristin VazzanaPriyanka RamKatja MohrsAmanda D’OrvilliersJenny XiaoSosina MakonnenCarlos HickeyCody ArnoldJason GiurleoYa Ping ChenCourtney ThwaitesDrew DudgeonKevin BrayAshique RafiqueTammy HuangFrank DelfinoAynur HermannJessica R. KirshnerMarc W. RetterRobert BabbDouglas MacDonaldGang ChenWilliam C. OlsonGavin ThurstonSamuel DavisJohn C. LinEric SmithNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Lauric Haber Kara Olson Marcus P. Kelly Alison Crawford David J. DiLillo Richard Tavaré Erica Ullman Shu Mao Lauren Canova Olga Sineshchekova Jennifer Finney Arpita Pawashe Supriya Patel Ryan McKay Sahar Rizvi Ermelinda Damko Danica Chiu Kristin Vazzana Priyanka Ram Katja Mohrs Amanda D’Orvilliers Jenny Xiao Sosina Makonnen Carlos Hickey Cody Arnold Jason Giurleo Ya Ping Chen Courtney Thwaites Drew Dudgeon Kevin Bray Ashique Rafique Tammy Huang Frank Delfino Aynur Hermann Jessica R. Kirshner Marc W. Retter Robert Babb Douglas MacDonald Gang Chen William C. Olson Gavin Thurston Samuel Davis John C. Lin Eric Smith Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| description |
Abstract T-cell-redirecting bispecific antibodies have emerged as a new class of therapeutic agents designed to simultaneously bind to T cells via CD3 and to tumor cells via tumor-cell-specific antigens (TSA), inducing T-cell-mediated killing of tumor cells. The promising preclinical and clinical efficacy of TSAxCD3 antibodies is often accompanied by toxicities such as cytokine release syndrome due to T-cell activation. How the efficacy and toxicity profile of the TSAxCD3 bispecific antibodies depends on the binding affinity to CD3 remains unclear. Here, we evaluate bispecific antibodies that were engineered to have a range of CD3 affinities, while retaining the same binding affinity for the selected tumor antigen. These agents were tested for their ability to kill tumor cells in vitro, and their biodistribution, serum half-life, and anti-tumor activity in vivo. Remarkably, by altering the binding affinity for CD3 alone, we can generate bispecific antibodies that maintain potent killing of TSA + tumor cells but display differential patterns of cytokine release, pharmacokinetics, and biodistribution. Therefore, tuning CD3 affinity is a promising method to improve the therapeutic index of T-cell-engaging bispecific antibodies. |
| format |
article |
| author |
Lauric Haber Kara Olson Marcus P. Kelly Alison Crawford David J. DiLillo Richard Tavaré Erica Ullman Shu Mao Lauren Canova Olga Sineshchekova Jennifer Finney Arpita Pawashe Supriya Patel Ryan McKay Sahar Rizvi Ermelinda Damko Danica Chiu Kristin Vazzana Priyanka Ram Katja Mohrs Amanda D’Orvilliers Jenny Xiao Sosina Makonnen Carlos Hickey Cody Arnold Jason Giurleo Ya Ping Chen Courtney Thwaites Drew Dudgeon Kevin Bray Ashique Rafique Tammy Huang Frank Delfino Aynur Hermann Jessica R. Kirshner Marc W. Retter Robert Babb Douglas MacDonald Gang Chen William C. Olson Gavin Thurston Samuel Davis John C. Lin Eric Smith |
| author_facet |
Lauric Haber Kara Olson Marcus P. Kelly Alison Crawford David J. DiLillo Richard Tavaré Erica Ullman Shu Mao Lauren Canova Olga Sineshchekova Jennifer Finney Arpita Pawashe Supriya Patel Ryan McKay Sahar Rizvi Ermelinda Damko Danica Chiu Kristin Vazzana Priyanka Ram Katja Mohrs Amanda D’Orvilliers Jenny Xiao Sosina Makonnen Carlos Hickey Cody Arnold Jason Giurleo Ya Ping Chen Courtney Thwaites Drew Dudgeon Kevin Bray Ashique Rafique Tammy Huang Frank Delfino Aynur Hermann Jessica R. Kirshner Marc W. Retter Robert Babb Douglas MacDonald Gang Chen William C. Olson Gavin Thurston Samuel Davis John C. Lin Eric Smith |
| author_sort |
Lauric Haber |
| title |
Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| title_short |
Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| title_full |
Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| title_fullStr |
Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| title_full_unstemmed |
Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning |
| title_sort |
generation of t-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by cd3 affinity tuning |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/13db8e3e17e043ffbbadbbb6bb988d63 |
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