Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant Staphylococcus aureus, Streptococcus dysgalactiae and Streptococcus uberis Causing Bovine Mastitis

Antibiotics are the most effective strategy to prevent and treat intramammary infections. However, their misuse has led to the dissemination of multidrug resistant bacteria (MDR) for both animals and humans. Efforts to develop new alternative strategies to control bacterial infections related to MDR...

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Autores principales:	Samantha Bennett, Laila Ben Said, Pierre Lacasse, François Malouin, Ismail Fliss
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotic resistance bacteriocin dairy cows intramammary infection alternative to antibiotics reuterin Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/13df85513dbd42c1ad5816455f4b092d
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spelling	oai:doaj.org-article:13df85513dbd42c1ad5816455f4b092d2021-11-25T16:25:06ZSusceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis10.3390/antibiotics101114182079-6382https://doaj.org/article/13df85513dbd42c1ad5816455f4b092d2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1418https://doaj.org/toc/2079-6382Antibiotics are the most effective strategy to prevent and treat intramammary infections. However, their misuse has led to the dissemination of multidrug resistant bacteria (MDR) for both animals and humans. Efforts to develop new alternative strategies to control bacterial infections related to MDR are continuously on the rise. The objective of this study was to evaluate the antimicrobial activity of different bacteriocins and reuterin against MDR <i>Staphylococcus</i> and <i>Streptococcus</i> clinical isolates involved in bovine mastitis. A bacterial collection including <i>S. aureus</i> (<i>n</i> = 19), <i>S. dysgalactiae</i> (<i>n</i> = 17) and <i>S. uberis</i> (<i>n</i> = 19) was assembled for this study. Antibiotic resistance profiles were determined by the disk diffusion method. In addition, sensitivity to bacteriocins and reuterin was evaluated by determining minimum inhibitory concentrations (MIC). A total of 21 strains (37.5%) were MDR. MICs ranged from ≤1.0 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL to ≥100 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL for nisin and 2.0 to ≥250 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL for bactofencin. Reuterin was active against all tested bacteria, and MICs vary between 70 and 560 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL. Interestingly, 20 MDR strains were inhibited by bactofencin at a concentration of ≤250 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL, while 14 were inhibited by nisin at an MIC of ≤100 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL. Pediocin did not show an inhibitory effect.Samantha BennettLaila Ben SaidPierre LacasseFrançois MalouinIsmail FlissMDPI AGarticleantibiotic resistancebacteriocindairy cowsintramammary infectionalternative to antibioticsreuterinTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1418, p 1418 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance bacteriocin dairy cows intramammary infection alternative to antibiotics reuterin Therapeutics. Pharmacology RM1-950
spellingShingle	antibiotic resistance bacteriocin dairy cows intramammary infection alternative to antibiotics reuterin Therapeutics. Pharmacology RM1-950 Samantha Bennett Laila Ben Said Pierre Lacasse François Malouin Ismail Fliss Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
description	Antibiotics are the most effective strategy to prevent and treat intramammary infections. However, their misuse has led to the dissemination of multidrug resistant bacteria (MDR) for both animals and humans. Efforts to develop new alternative strategies to control bacterial infections related to MDR are continuously on the rise. The objective of this study was to evaluate the antimicrobial activity of different bacteriocins and reuterin against MDR <i>Staphylococcus</i> and <i>Streptococcus</i> clinical isolates involved in bovine mastitis. A bacterial collection including <i>S. aureus</i> (<i>n</i> = 19), <i>S. dysgalactiae</i> (<i>n</i> = 17) and <i>S. uberis</i> (<i>n</i> = 19) was assembled for this study. Antibiotic resistance profiles were determined by the disk diffusion method. In addition, sensitivity to bacteriocins and reuterin was evaluated by determining minimum inhibitory concentrations (MIC). A total of 21 strains (37.5%) were MDR. MICs ranged from ≤1.0 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL to ≥100 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL for nisin and 2.0 to ≥250 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL for bactofencin. Reuterin was active against all tested bacteria, and MICs vary between 70 and 560 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL. Interestingly, 20 MDR strains were inhibited by bactofencin at a concentration of ≤250 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL, while 14 were inhibited by nisin at an MIC of ≤100 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="sans-serif">μ</mi></semantics></math></inline-formula>g/mL. Pediocin did not show an inhibitory effect.
format	article
author	Samantha Bennett Laila Ben Said Pierre Lacasse François Malouin Ismail Fliss
author_facet	Samantha Bennett Laila Ben Said Pierre Lacasse François Malouin Ismail Fliss
author_sort	Samantha Bennett
title	Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
title_short	Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
title_full	Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
title_fullStr	Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
title_full_unstemmed	Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis
title_sort	susceptibility to nisin, bactofencin, pediocin and reuterin of multidrug resistant <i>staphylococcus aureus</i>, <i>streptococcus dysgalactiae</i> and <i>streptococcus uberis</i> causing bovine mastitis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/13df85513dbd42c1ad5816455f4b092d
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Susceptibility to Nisin, Bactofencin, Pediocin and Reuterin of Multidrug Resistant <i>Staphylococcus aureus</i>, <i>Streptococcus dysgalactiae</i> and <i>Streptococcus uberis</i> Causing Bovine Mastitis

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