Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities

Abstract. Trianto A, Radjasa OK, Subagiyo, Purnaweni H, Bahry MS, Djamaludin R, Tjoa A, Singleton I, Diele K, Evan D. 2021. Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities. Biodiversitas 22: 1717- 1724. The hi...

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Autores principales:	AGUS TRIANTO, OCKY KARNA RADJASA, SUBAGIYO SUBAGIYO, HARTUTI PURNAWENI, MUHAMMAD SYAIFUDIEN BAHRY, RIGNOLDA DJAMALUDIN, AIYEN TJOA, IAN SINGLETON, KAREN DIELE, DARREN EVAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	fungi, mangrove, protease, cellulose, anti-microbial activity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/13e1fc1befb448bdb436e50b9b0f9393
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spelling	oai:doaj.org-article:13e1fc1befb448bdb436e50b9b0f93932021-11-22T00:57:38ZPotential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities1412-033X2085-472210.13057/biodiv/d220415https://doaj.org/article/13e1fc1befb448bdb436e50b9b0f93932021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7791https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Trianto A, Radjasa OK, Subagiyo, Purnaweni H, Bahry MS, Djamaludin R, Tjoa A, Singleton I, Diele K, Evan D. 2021. Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities. Biodiversitas 22: 1717- 1724. The high and relatively unexplored diversity of fungi present in the mangrove ecosystem represents a source of novel biotechnological importance. This study explored the potential of fungi isolated from the mangrove ecosystems to produce proteases and cellulases (commercially important enzymes) and their ability to inhibit pathogenic Vibrio species. Random samples of root, branch, leaf, sediments and litters were collected from 5 different mangrove sites in Manado, North Sulawesi, as a source of fungal isolates. The fungi were isolated on malt extract agar (MEA) and potato dextrose agar (PDA). The isolates were identified mainly based on the molecular methods (18S gene sequence) and examined for their ability to produce proteases, cellulases, and activity against several Vibrio species. Altogether 288 species of fungi were isolated from all samples. The fungi, isolated from leaves showed the highest diversity. A fungal isolate 19 Mba-C2-1 Fusarium equiseti from Avicennia sp. leaf showed the highest protease activity. While, the isolate 19 MT-05-3 Hypocrea sp. from sediment had the highest cellulase activity. From the root of Rhizophora sp., the isolate 19 MT-04-3 identified as Trichoderma viride had the strongest activity against a range of Vibrio species. This work indicates the high potential of fungi isolated from mangrove ecosystems as a source of commercially important enzymes and novel antimicrobial compounds.AGUS TRIANTOOCKY KARNA RADJASASUBAGIYO SUBAGIYOHARTUTI PURNAWENIMUHAMMAD SYAIFUDIEN BAHRYRIGNOLDA DJAMALUDINAIYEN TJOAIAN SINGLETONKAREN DIELEDARREN EVANMBI & UNS Soloarticlefungi, mangrove, protease, cellulose, anti-microbial activityBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fungi, mangrove, protease, cellulose, anti-microbial activity Biology (General) QH301-705.5
spellingShingle	fungi, mangrove, protease, cellulose, anti-microbial activity Biology (General) QH301-705.5 AGUS TRIANTO OCKY KARNA RADJASA SUBAGIYO SUBAGIYO HARTUTI PURNAWENI MUHAMMAD SYAIFUDIEN BAHRY RIGNOLDA DJAMALUDIN AIYEN TJOA IAN SINGLETON KAREN DIELE DARREN EVAN Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
description	Abstract. Trianto A, Radjasa OK, Subagiyo, Purnaweni H, Bahry MS, Djamaludin R, Tjoa A, Singleton I, Diele K, Evan D. 2021. Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities. Biodiversitas 22: 1717- 1724. The high and relatively unexplored diversity of fungi present in the mangrove ecosystem represents a source of novel biotechnological importance. This study explored the potential of fungi isolated from the mangrove ecosystems to produce proteases and cellulases (commercially important enzymes) and their ability to inhibit pathogenic Vibrio species. Random samples of root, branch, leaf, sediments and litters were collected from 5 different mangrove sites in Manado, North Sulawesi, as a source of fungal isolates. The fungi were isolated on malt extract agar (MEA) and potato dextrose agar (PDA). The isolates were identified mainly based on the molecular methods (18S gene sequence) and examined for their ability to produce proteases, cellulases, and activity against several Vibrio species. Altogether 288 species of fungi were isolated from all samples. The fungi, isolated from leaves showed the highest diversity. A fungal isolate 19 Mba-C2-1 Fusarium equiseti from Avicennia sp. leaf showed the highest protease activity. While, the isolate 19 MT-05-3 Hypocrea sp. from sediment had the highest cellulase activity. From the root of Rhizophora sp., the isolate 19 MT-04-3 identified as Trichoderma viride had the strongest activity against a range of Vibrio species. This work indicates the high potential of fungi isolated from mangrove ecosystems as a source of commercially important enzymes and novel antimicrobial compounds.
format	article
author	AGUS TRIANTO OCKY KARNA RADJASA SUBAGIYO SUBAGIYO HARTUTI PURNAWENI MUHAMMAD SYAIFUDIEN BAHRY RIGNOLDA DJAMALUDIN AIYEN TJOA IAN SINGLETON KAREN DIELE DARREN EVAN
author_facet	AGUS TRIANTO OCKY KARNA RADJASA SUBAGIYO SUBAGIYO HARTUTI PURNAWENI MUHAMMAD SYAIFUDIEN BAHRY RIGNOLDA DJAMALUDIN AIYEN TJOA IAN SINGLETON KAREN DIELE DARREN EVAN
author_sort	AGUS TRIANTO
title	Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
title_short	Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
title_full	Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
title_fullStr	Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
title_full_unstemmed	Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities
title_sort	potential of fungi isolated from a mangrove ecosystem in northern sulawesi, indonesia: protease, cellulase and anti-microbial capabilities
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/13e1fc1befb448bdb436e50b9b0f9393
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Potential of fungi isolated from a mangrove ecosystem in Northern Sulawesi, Indonesia: Protease, cellulase and anti-microbial capabilities

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