Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis

Colorectal cancer (CRC) is ranked as the third most common malignancy worldwide. Therefore, it is urgent to screen novel and effective molecular drug targets for colorectal cancer therapeutics. In this study, the specific role and related mechanism underlying Ring finger (RNF) 220 in colon cancer we...

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Autores principales:	Jianwen Yan, Min Tan, Lin Yu, Xichao Jin, Yangcheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	rnf220 usp22 bmi1 stemness progression colon cancer Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/13ea0175b17b4f14af014f452a64ac39
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spelling	oai:doaj.org-article:13ea0175b17b4f14af014f452a64ac392021-11-11T14:23:43ZRing finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis2165-59792165-598710.1080/21655979.2021.2003664https://doaj.org/article/13ea0175b17b4f14af014f452a64ac392021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2003664https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Colorectal cancer (CRC) is ranked as the third most common malignancy worldwide. Therefore, it is urgent to screen novel and effective molecular drug targets for colorectal cancer therapeutics. In this study, the specific role and related mechanism underlying Ring finger (RNF) 220 in colon cancer were investigated. Firstly, RT-PCR assay was used to compare differences between expression levels of RNF220 in colorectal tumor and normal tissues. Western blot and RT-PCR assays were applied to examine the protein levels of RNF220 in normal colonic mucosa and colorectal cancer cells. We found that RNF220 was upregulated in colorectal cancer in patients and cell models. RNF220 promoted the proliferation and migration, invasion of colorectal cancer cells through BrdU incorporation, clone formation, transwell and wound healing assays. Spheroid formation and western blot assays illustrated that RNF220 promoted the stemness of colorectal cancer cells. Moreover, we found that RNF220 regulated BMI1 expression through USP22 by western blot. Finally, we discovered that RNF220 facilitated tumor growth in vivo through establishment of subcutaneous xenograft tumor mice model. In conclusion, RNF220 promoted the stemness and progression of colon cancer cells via the USP22-BMI1 axis.Jianwen YanMin TanLin YuXichao JinYangcheng LiTaylor & Francis Grouparticlernf220usp22bmi1stemnessprogressioncolon cancerBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rnf220 usp22 bmi1 stemness progression colon cancer Biotechnology TP248.13-248.65
spellingShingle	rnf220 usp22 bmi1 stemness progression colon cancer Biotechnology TP248.13-248.65 Jianwen Yan Min Tan Lin Yu Xichao Jin Yangcheng Li Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
description	Colorectal cancer (CRC) is ranked as the third most common malignancy worldwide. Therefore, it is urgent to screen novel and effective molecular drug targets for colorectal cancer therapeutics. In this study, the specific role and related mechanism underlying Ring finger (RNF) 220 in colon cancer were investigated. Firstly, RT-PCR assay was used to compare differences between expression levels of RNF220 in colorectal tumor and normal tissues. Western blot and RT-PCR assays were applied to examine the protein levels of RNF220 in normal colonic mucosa and colorectal cancer cells. We found that RNF220 was upregulated in colorectal cancer in patients and cell models. RNF220 promoted the proliferation and migration, invasion of colorectal cancer cells through BrdU incorporation, clone formation, transwell and wound healing assays. Spheroid formation and western blot assays illustrated that RNF220 promoted the stemness of colorectal cancer cells. Moreover, we found that RNF220 regulated BMI1 expression through USP22 by western blot. Finally, we discovered that RNF220 facilitated tumor growth in vivo through establishment of subcutaneous xenograft tumor mice model. In conclusion, RNF220 promoted the stemness and progression of colon cancer cells via the USP22-BMI1 axis.
format	article
author	Jianwen Yan Min Tan Lin Yu Xichao Jin Yangcheng Li
author_facet	Jianwen Yan Min Tan Lin Yu Xichao Jin Yangcheng Li
author_sort	Jianwen Yan
title	Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
title_short	Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
title_full	Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
title_fullStr	Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
title_full_unstemmed	Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis
title_sort	ring finger 220 promotes the stemness and progression of colon cancer cells via ubiquitin specific peptidase 22-bmi1 axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/13ea0175b17b4f14af014f452a64ac39
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Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis

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