Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci

Abstract Chlamydia gallinacea is an obligate intracellular bacterium that has recently been added to the family of Chlamydiaceae. C. gallinacea is genetically diverse, widespread in poultry and a suspected cause of pneumonia in slaughterhouse workers. In poultry, C. gallinacea infections appear asym...

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Autores principales:	Marloes Heijne, Martina Jelocnik, Alexander Umanets, Michael S. M. Brouwer, Annemieke Dinkla, Frank Harders, Lucien J. M. van Keulen, Hendrik Jan Roest, Famke Schaafsma, Francisca C. Velkers, Jeanet A. van der Goot, Yvonne Pannekoek, Ad P. Koets
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13ee2af1fea3462987074f5ff3d377cf
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spelling	oai:doaj.org-article:13ee2af1fea3462987074f5ff3d377cf2021-12-02T18:50:59ZGenetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci10.1038/s41598-021-95966-92045-2322https://doaj.org/article/13ee2af1fea3462987074f5ff3d377cf2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95966-9https://doaj.org/toc/2045-2322Abstract Chlamydia gallinacea is an obligate intracellular bacterium that has recently been added to the family of Chlamydiaceae. C. gallinacea is genetically diverse, widespread in poultry and a suspected cause of pneumonia in slaughterhouse workers. In poultry, C. gallinacea infections appear asymptomatic, but studies about the pathogenic potential are limited. In this study two novel sequence types of C. gallinacea were isolated from apparently healthy chickens. Both isolates (NL_G47 and NL_F725) were closely related to each other and have at least 99.5% DNA sequence identity to C. gallinacea Type strain 08-1274/3. To gain further insight into the pathogenic potential, infection experiments in embryonated chicken eggs and comparative genomics with Chlamydia psittaci were performed. C. psittaci is a ubiquitous zoonotic pathogen of birds and mammals, and infection in poultry can result in severe systemic illness. In experiments with embryonated chicken eggs, C. gallinacea induced mortality was observed, potentially strain dependent, but lower compared to C. psittaci induced mortality. Comparative analyses confirmed all currently available C. gallinacea genomes possess the hallmark genes coding for known and potential virulence factors as found in C. psittaci albeit to a reduced number of orthologues or paralogs. The presence of potential virulence factors and the observed mortality in embryonated eggs indicates C. gallinacea should rather be considered as an opportunistic pathogen than an innocuous commensal.Marloes HeijneMartina JelocnikAlexander UmanetsMichael S. M. BrouwerAnnemieke DinklaFrank HardersLucien J. M. van KeulenHendrik Jan RoestFamke SchaafsmaFrancisca C. VelkersJeanet A. van der GootYvonne PannekoekAd P. KoetsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marloes Heijne Martina Jelocnik Alexander Umanets Michael S. M. Brouwer Annemieke Dinkla Frank Harders Lucien J. M. van Keulen Hendrik Jan Roest Famke Schaafsma Francisca C. Velkers Jeanet A. van der Goot Yvonne Pannekoek Ad P. Koets Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
description	Abstract Chlamydia gallinacea is an obligate intracellular bacterium that has recently been added to the family of Chlamydiaceae. C. gallinacea is genetically diverse, widespread in poultry and a suspected cause of pneumonia in slaughterhouse workers. In poultry, C. gallinacea infections appear asymptomatic, but studies about the pathogenic potential are limited. In this study two novel sequence types of C. gallinacea were isolated from apparently healthy chickens. Both isolates (NL_G47 and NL_F725) were closely related to each other and have at least 99.5% DNA sequence identity to C. gallinacea Type strain 08-1274/3. To gain further insight into the pathogenic potential, infection experiments in embryonated chicken eggs and comparative genomics with Chlamydia psittaci were performed. C. psittaci is a ubiquitous zoonotic pathogen of birds and mammals, and infection in poultry can result in severe systemic illness. In experiments with embryonated chicken eggs, C. gallinacea induced mortality was observed, potentially strain dependent, but lower compared to C. psittaci induced mortality. Comparative analyses confirmed all currently available C. gallinacea genomes possess the hallmark genes coding for known and potential virulence factors as found in C. psittaci albeit to a reduced number of orthologues or paralogs. The presence of potential virulence factors and the observed mortality in embryonated eggs indicates C. gallinacea should rather be considered as an opportunistic pathogen than an innocuous commensal.
format	article
author	Marloes Heijne Martina Jelocnik Alexander Umanets Michael S. M. Brouwer Annemieke Dinkla Frank Harders Lucien J. M. van Keulen Hendrik Jan Roest Famke Schaafsma Francisca C. Velkers Jeanet A. van der Goot Yvonne Pannekoek Ad P. Koets
author_facet	Marloes Heijne Martina Jelocnik Alexander Umanets Michael S. M. Brouwer Annemieke Dinkla Frank Harders Lucien J. M. van Keulen Hendrik Jan Roest Famke Schaafsma Francisca C. Velkers Jeanet A. van der Goot Yvonne Pannekoek Ad P. Koets
author_sort	Marloes Heijne
title	Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
title_short	Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
title_full	Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
title_fullStr	Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
title_full_unstemmed	Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci
title_sort	genetic and phenotypic analysis of the pathogenic potential of two novel chlamydia gallinacea strains compared to chlamydia psittaci
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/13ee2af1fea3462987074f5ff3d377cf
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Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci

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