Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China

ABSTRACT Père David’s deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and prov...

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Autores principales:	Xiaoyu Lu, Xia Xiao, Yuan Liu, Siyang Huang, Ruichao Li, Zhiqiang Wang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Escherichia coli IncI2 Père David's deer blaCTX-M-132 colistin resistance mcr-1 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/13f148aaf6844c19a90207917bb244d8
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spelling	oai:doaj.org-article:13f148aaf6844c19a90207917bb244d82021-11-15T15:31:13ZWidespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China10.1128/mSphere.01221-202379-5042https://doaj.org/article/13f148aaf6844c19a90207917bb244d82020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.01221-20https://doaj.org/toc/2379-5042ABSTRACT Père David’s deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemination of AMR in nature reserve settings. Sixty-seven mcr-1-positive Escherichia coli isolates from 97 fecal samples were identified by PCR and found resistant to colistin. The prevalence of β-lactam resistance was very high, and there were 64 mcr-1-positive isolates containing β-lactamase genes. Transconjugants of 66 mcr-1-positive isolates were acquired through conjugation experiments. PCR-based replicon typing (PBRT) showed that 44 strains harbored IncI2 mcr-1-bearing plasmids, eight strains harbored IncX4 mcr-1-carrying plasmids, and 14 strains harbored IncHI2 mcr-1-positive plasmids. Notably, mcr-1 was located in the chromosome of LD27-1. Clonal dissemination and horizontal dissemination of mcr-1 by plasmids coexist. We first report the prevalence of plasmid-mediated mcr-1 in E. coli from Père David’s deer in China. mcr-1-bearing IncI2 plasmid was the most frequent plasmid type, and the first IncI2 plasmid harboring both blaCTX-M-132 and mcr-1 is characterized here. Our results support the implication of Père David’s deer as a potential reservoir for MCR-1-producing E. coli. IMPORTANCE The mcr-1 gene is widely reported around the world and has been identified on various plasmids with different replicon types. Resistance to the last-line antibiotic colistin mediated by mcr-1 still represents a threat to global public health. Père David’s deer is a highly endangered species originating in China, and many deer are currently being raised in captivity for gradual reintroduction to the wild. If this species carries AMR bacteria, it will pose a potential threat to the environment. Therefore, research on the dissemination of mcr-1-positive E. coli from Père David’s deer is of great significance. This is the first study to investigate the microbiological and genomic surveillance of MCR-1-producing bacteria colonized among Père David’s deer in China, and we uncovered a high prevalence of MCR-1-producing E. coli. The importance of constant surveillance for AMR bacteria in nature reserve settings is emphasized.Xiaoyu LuXia XiaoYuan LiuSiyang HuangRuichao LiZhiqiang WangAmerican Society for MicrobiologyarticleEscherichia coliIncI2Père David's deerblaCTX-M-132colistin resistancemcr-1MicrobiologyQR1-502ENmSphere, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli IncI2 Père David's deer blaCTX-M-132 colistin resistance mcr-1 Microbiology QR1-502
spellingShingle	Escherichia coli IncI2 Père David's deer blaCTX-M-132 colistin resistance mcr-1 Microbiology QR1-502 Xiaoyu Lu Xia Xiao Yuan Liu Siyang Huang Ruichao Li Zhiqiang Wang Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
description	ABSTRACT Père David’s deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemination of AMR in nature reserve settings. Sixty-seven mcr-1-positive Escherichia coli isolates from 97 fecal samples were identified by PCR and found resistant to colistin. The prevalence of β-lactam resistance was very high, and there were 64 mcr-1-positive isolates containing β-lactamase genes. Transconjugants of 66 mcr-1-positive isolates were acquired through conjugation experiments. PCR-based replicon typing (PBRT) showed that 44 strains harbored IncI2 mcr-1-bearing plasmids, eight strains harbored IncX4 mcr-1-carrying plasmids, and 14 strains harbored IncHI2 mcr-1-positive plasmids. Notably, mcr-1 was located in the chromosome of LD27-1. Clonal dissemination and horizontal dissemination of mcr-1 by plasmids coexist. We first report the prevalence of plasmid-mediated mcr-1 in E. coli from Père David’s deer in China. mcr-1-bearing IncI2 plasmid was the most frequent plasmid type, and the first IncI2 plasmid harboring both blaCTX-M-132 and mcr-1 is characterized here. Our results support the implication of Père David’s deer as a potential reservoir for MCR-1-producing E. coli. IMPORTANCE The mcr-1 gene is widely reported around the world and has been identified on various plasmids with different replicon types. Resistance to the last-line antibiotic colistin mediated by mcr-1 still represents a threat to global public health. Père David’s deer is a highly endangered species originating in China, and many deer are currently being raised in captivity for gradual reintroduction to the wild. If this species carries AMR bacteria, it will pose a potential threat to the environment. Therefore, research on the dissemination of mcr-1-positive E. coli from Père David’s deer is of great significance. This is the first study to investigate the microbiological and genomic surveillance of MCR-1-producing bacteria colonized among Père David’s deer in China, and we uncovered a high prevalence of MCR-1-producing E. coli. The importance of constant surveillance for AMR bacteria in nature reserve settings is emphasized.
format	article
author	Xiaoyu Lu Xia Xiao Yuan Liu Siyang Huang Ruichao Li Zhiqiang Wang
author_facet	Xiaoyu Lu Xia Xiao Yuan Liu Siyang Huang Ruichao Li Zhiqiang Wang
author_sort	Xiaoyu Lu
title	Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
title_short	Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
title_full	Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
title_fullStr	Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
title_full_unstemmed	Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China
title_sort	widespread prevalence of plasmid-mediated colistin resistance gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">escherichia coli</named-content> from père david's deer in china
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/13f148aaf6844c19a90207917bb244d8
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Widespread Prevalence of Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-1</italic> in <named-content content-type="genus-species">Escherichia coli</named-content> from Père David's Deer in China

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