A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections

Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liv...

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Autores principales:	Susumu Tadokoro, Reiko Tokuyama-Toda, Seiko Tatehara, Shinji Ide, Hirochika Umeki, Keiko Miyoshi, Takafumi Noma, Kazuhito Satomura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iPSC differentiation frozen section Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/13f33f043a324e809017b77474f3db2d
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spelling	oai:doaj.org-article:13f33f043a324e809017b77474f3db2d2021-11-25T17:07:40ZA New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections10.3390/cells101128272073-4409https://doaj.org/article/13f33f043a324e809017b77474f3db2d2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2827https://doaj.org/toc/2073-4409Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality.Susumu TadokoroReiko Tokuyama-TodaSeiko TateharaShinji IdeHirochika UmekiKeiko MiyoshiTakafumi NomaKazuhito SatomuraMDPI AGarticleiPSCdifferentiationfrozen sectionBiology (General)QH301-705.5ENCells, Vol 10, Iss 2827, p 2827 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iPSC differentiation frozen section Biology (General) QH301-705.5
spellingShingle	iPSC differentiation frozen section Biology (General) QH301-705.5 Susumu Tadokoro Reiko Tokuyama-Toda Seiko Tatehara Shinji Ide Hirochika Umeki Keiko Miyoshi Takafumi Noma Kazuhito Satomura A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
description	Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality.
format	article
author	Susumu Tadokoro Reiko Tokuyama-Toda Seiko Tatehara Shinji Ide Hirochika Umeki Keiko Miyoshi Takafumi Noma Kazuhito Satomura
author_facet	Susumu Tadokoro Reiko Tokuyama-Toda Seiko Tatehara Shinji Ide Hirochika Umeki Keiko Miyoshi Takafumi Noma Kazuhito Satomura
author_sort	Susumu Tadokoro
title	A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
title_short	A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
title_full	A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
title_fullStr	A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
title_full_unstemmed	A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections
title_sort	new induction method for the controlled differentiation of human-induced pluripotent stem cells using frozen sections
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/13f33f043a324e809017b77474f3db2d
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A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections

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