Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli

Abstract Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during repl...

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Autores principales:	Emily Helgesen, Frank Sætre, Kirsten Skarstad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13fcba975ef245dcaeeacf34ab761619
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spelling	oai:doaj.org-article:13fcba975ef245dcaeeacf34ab7616192021-12-02T15:22:59ZTopoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli10.1038/s41598-020-80043-42045-2322https://doaj.org/article/13fcba975ef245dcaeeacf34ab7616192021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80043-4https://doaj.org/toc/2045-2322Abstract Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.Emily HelgesenFrank SætreKirsten SkarstadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emily Helgesen Frank Sætre Kirsten Skarstad Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
description	Abstract Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.
format	article
author	Emily Helgesen Frank Sætre Kirsten Skarstad
author_facet	Emily Helgesen Frank Sætre Kirsten Skarstad
author_sort	Emily Helgesen
title	Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_short	Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_full	Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_fullStr	Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_full_unstemmed	Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
title_sort	topoisomerase iv tracks behind the replication fork and the seqa complex during dna replication in escherichia coli
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/13fcba975ef245dcaeeacf34ab761619
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Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli

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