LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize...

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Autores principales:	Akhtar Ali, Yasmeen M. Bashmil, Jeremy J. Cottrell, Hafiz A. R. Suleria, Frank R. Dunshea
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	herbs medicinal plants polyphenols antioxidants human health LC-MS/MS Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/14030538cc3642e785755178b7ea40e0
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spelling	oai:doaj.org-article:14030538cc3642e785755178b7ea40e02021-11-25T16:28:07ZLC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential10.3390/antiox101117702076-3921https://doaj.org/article/14030538cc3642e785755178b7ea40e02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1770https://doaj.org/toc/2076-3921Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.Akhtar AliYasmeen M. BashmilJeremy J. CottrellHafiz A. R. SuleriaFrank R. DunsheaMDPI AGarticleherbsmedicinal plantspolyphenolsantioxidantshuman healthLC-MS/MSTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1770, p 1770 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	herbs medicinal plants polyphenols antioxidants human health LC-MS/MS Therapeutics. Pharmacology RM1-950
spellingShingle	herbs medicinal plants polyphenols antioxidants human health LC-MS/MS Therapeutics. Pharmacology RM1-950 Akhtar Ali Yasmeen M. Bashmil Jeremy J. Cottrell Hafiz A. R. Suleria Frank R. Dunshea LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
description	Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.
format	article
author	Akhtar Ali Yasmeen M. Bashmil Jeremy J. Cottrell Hafiz A. R. Suleria Frank R. Dunshea
author_facet	Akhtar Ali Yasmeen M. Bashmil Jeremy J. Cottrell Hafiz A. R. Suleria Frank R. Dunshea
author_sort	Akhtar Ali
title	LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
title_short	LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
title_full	LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
title_fullStr	LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
title_full_unstemmed	LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential
title_sort	lc-ms/ms-qtof screening and identification of phenolic compounds from australian grown herbs and their antioxidant potential
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/14030538cc3642e785755178b7ea40e0
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LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

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