Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry

Abstract Direct mass spectrometry analysis of metabolic effects of clenbuterol and salbutamol on pork quality at the molecular level is incredibly beneficial for food regulations, public health and the development of new anti-obesity drugs. With internal extractive electrospray ionization mass spect...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haiyan Lu, Hua Zhang, Tenggao Zhu, Yipo Xiao, Shaoxian Xie, Haiwei Gu, Meng Cui, Liping Luo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14054b9e87e44c408082e35298124a88
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:14054b9e87e44c408082e35298124a88
record_format	dspace
spelling	oai:doaj.org-article:14054b9e87e44c408082e35298124a882021-12-02T15:05:54ZMetabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry10.1038/s41598-017-05496-62045-2322https://doaj.org/article/14054b9e87e44c408082e35298124a882017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05496-6https://doaj.org/toc/2045-2322Abstract Direct mass spectrometry analysis of metabolic effects of clenbuterol and salbutamol on pork quality at the molecular level is incredibly beneficial for food regulations, public health and the development of new anti-obesity drugs. With internal extractive electrospray ionization mass spectrometry (iEESI-MS), nutrients including creatine, amino acids, L-carnitine, vitamin B6, carnosine and phosphatidylcholines in pork tissue were identified, without sample pretreatment, using collision-induced dissociation (CID) experiments and by comparison with authentic compounds. Furthermore, normal pork samples were clearly differentiated from pork samples with clenbuterol and salbutamol via principal component analysis (PCA). Correlation analysis performed on the spectral data revealed that the above-mentioned nutrients strongly correlated with pork quality, and the absolute intensity of phosphatidylcholines in normal pork was much higher than pork contaminated by clenbuterol and salbutamol. Our findings suggested that clenbuterol and salbutamol may render effects on the activity of carnitine acyltransferase I, hence the process that L-carnitine transports long-chain fatty acids into mitochondria and the formation of phosphatidylcholines might be affected. However, the underlying metabolic mechanisms of clenbuterol and salbutamol on carnitine acyltransferase I requires more comprehensive studies in future work.Haiyan LuHua ZhangTenggao ZhuYipo XiaoShaoxian XieHaiwei GuMeng CuiLiping LuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haiyan Lu Hua Zhang Tenggao Zhu Yipo Xiao Shaoxian Xie Haiwei Gu Meng Cui Liping Luo Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
description	Abstract Direct mass spectrometry analysis of metabolic effects of clenbuterol and salbutamol on pork quality at the molecular level is incredibly beneficial for food regulations, public health and the development of new anti-obesity drugs. With internal extractive electrospray ionization mass spectrometry (iEESI-MS), nutrients including creatine, amino acids, L-carnitine, vitamin B6, carnosine and phosphatidylcholines in pork tissue were identified, without sample pretreatment, using collision-induced dissociation (CID) experiments and by comparison with authentic compounds. Furthermore, normal pork samples were clearly differentiated from pork samples with clenbuterol and salbutamol via principal component analysis (PCA). Correlation analysis performed on the spectral data revealed that the above-mentioned nutrients strongly correlated with pork quality, and the absolute intensity of phosphatidylcholines in normal pork was much higher than pork contaminated by clenbuterol and salbutamol. Our findings suggested that clenbuterol and salbutamol may render effects on the activity of carnitine acyltransferase I, hence the process that L-carnitine transports long-chain fatty acids into mitochondria and the formation of phosphatidylcholines might be affected. However, the underlying metabolic mechanisms of clenbuterol and salbutamol on carnitine acyltransferase I requires more comprehensive studies in future work.
format	article
author	Haiyan Lu Hua Zhang Tenggao Zhu Yipo Xiao Shaoxian Xie Haiwei Gu Meng Cui Liping Luo
author_facet	Haiyan Lu Hua Zhang Tenggao Zhu Yipo Xiao Shaoxian Xie Haiwei Gu Meng Cui Liping Luo
author_sort	Haiyan Lu
title	Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
title_short	Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
title_full	Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
title_fullStr	Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
title_full_unstemmed	Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry
title_sort	metabolic effects of clenbuterol and salbutamol on pork meat studied using internal extractive electrospray ionization mass spectrometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/14054b9e87e44c408082e35298124a88
work_keys_str_mv	AT haiyanlu metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT huazhang metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT tenggaozhu metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT yipoxiao metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT shaoxianxie metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT haiweigu metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT mengcui metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry AT lipingluo metaboliceffectsofclenbuterolandsalbutamolonporkmeatstudiedusinginternalextractiveelectrosprayionizationmassspectrometry
_version_	1718388627184025600

Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry

Ejemplares similares