Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial

Abstract This study aimed to measure hemolysis before, during and after 60 days of the ground-based spaceflight analog bed rest and the effect of a nutritional intervention through a prospective randomized clinical trial. Twenty male participants were hospitalized for 88 days comprised of 14 days of...

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Autores principales:	Kathryn Culliton, Hakim Louati, Odette Laneuville, Tim Ramsay, Guy Trudel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/14076d88daca47929aeaeedad9ebfa25
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spelling	oai:doaj.org-article:14076d88daca47929aeaeedad9ebfa252021-12-02T12:11:05ZSix degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial10.1038/s41526-021-00132-02373-8065https://doaj.org/article/14076d88daca47929aeaeedad9ebfa252021-02-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00132-0https://doaj.org/toc/2373-8065Abstract This study aimed to measure hemolysis before, during and after 60 days of the ground-based spaceflight analog bed rest and the effect of a nutritional intervention through a prospective randomized clinical trial. Twenty male participants were hospitalized for 88 days comprised of 14 days of ambulatory baseline, 60 days of 6° head-down tilt bed rest and 14 days of reambulation. Ten participants each received a control diet or daily polyphenol associated with omega-3, vitamin E, and selenium supplements. The primary outcome was endogenous carbon monoxide (CO) elimination measured by gas chromatography. Hemolysis was also measured with serial bilirubin, iron, transferrin saturation blood levels and serial 3-day stool collections were used to measure urobilinoid excretion using photometry. Total hemoglobin mass (tHb) was measured using CO-rebreathing. CO elimination increased after 5, 11, 30, and 57 days of bed rest: +289 ppb (95% CI 101–477 ppb; p = 0.004), +253 ppb (78–427 ppb; p = 0.007), +193 ppb (89–298 ppb; p = 0.001) and +858 ppb (670–1046 ppb; p < 0.000), respectively, compared to baseline. Bilirubin increased after 20 and 49 days of bed rest +0.8 mg/l (p = 0.013) and +1.1 mg/l (p = 0.012), respectively; and iron increased after 20 days of bed rest +10.5 µg/dl (p = 0.032). The nutritional intervention did not change CO elimination. THb was lower after 60 days of bed rest −0.9 g/kg (p = 0.001). Bed rest enhanced hemolysis as measured through all three by-products of heme oxygenase. Ongoing enhanced hemolysis over 60 days contributed to a 10% decrease in tHb mass. Modulation of red blood cell control towards increased hemolysis may be an important mechanism causing anemia in astronauts.Kathryn CullitonHakim LouatiOdette LaneuvilleTim RamsayGuy TrudelNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Kathryn Culliton Hakim Louati Odette Laneuville Tim Ramsay Guy Trudel Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
description	Abstract This study aimed to measure hemolysis before, during and after 60 days of the ground-based spaceflight analog bed rest and the effect of a nutritional intervention through a prospective randomized clinical trial. Twenty male participants were hospitalized for 88 days comprised of 14 days of ambulatory baseline, 60 days of 6° head-down tilt bed rest and 14 days of reambulation. Ten participants each received a control diet or daily polyphenol associated with omega-3, vitamin E, and selenium supplements. The primary outcome was endogenous carbon monoxide (CO) elimination measured by gas chromatography. Hemolysis was also measured with serial bilirubin, iron, transferrin saturation blood levels and serial 3-day stool collections were used to measure urobilinoid excretion using photometry. Total hemoglobin mass (tHb) was measured using CO-rebreathing. CO elimination increased after 5, 11, 30, and 57 days of bed rest: +289 ppb (95% CI 101–477 ppb; p = 0.004), +253 ppb (78–427 ppb; p = 0.007), +193 ppb (89–298 ppb; p = 0.001) and +858 ppb (670–1046 ppb; p < 0.000), respectively, compared to baseline. Bilirubin increased after 20 and 49 days of bed rest +0.8 mg/l (p = 0.013) and +1.1 mg/l (p = 0.012), respectively; and iron increased after 20 days of bed rest +10.5 µg/dl (p = 0.032). The nutritional intervention did not change CO elimination. THb was lower after 60 days of bed rest −0.9 g/kg (p = 0.001). Bed rest enhanced hemolysis as measured through all three by-products of heme oxygenase. Ongoing enhanced hemolysis over 60 days contributed to a 10% decrease in tHb mass. Modulation of red blood cell control towards increased hemolysis may be an important mechanism causing anemia in astronauts.
format	article
author	Kathryn Culliton Hakim Louati Odette Laneuville Tim Ramsay Guy Trudel
author_facet	Kathryn Culliton Hakim Louati Odette Laneuville Tim Ramsay Guy Trudel
author_sort	Kathryn Culliton
title	Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
title_short	Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
title_full	Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
title_fullStr	Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
title_full_unstemmed	Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
title_sort	six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/14076d88daca47929aeaeedad9ebfa25
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Six degrees head-down tilt bed rest caused low-grade hemolysis: a prospective randomized clinical trial

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