Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing

Summary: Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA molecules from a single pre-mRNA with a prominent role in the development and function of the central nervous system. We used long-read isoform sequencing to generate full-length transcript sequences...

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Autores principales:	Szi Kay Leung, Aaron R. Jeffries, Isabel Castanho, Ben T. Jordan, Karen Moore, Jonathan P. Davies, Emma L. Dempster, Nicholas J. Bray, Paul O’Neill, Elizabeth Tseng, Zeshan Ahmed, David A. Collier, Erin D. Jeffery, Shyam Prabhakar, Leonard Schalkwyk, Connor Jops, Michael J. Gandal, Gloria M. Sheynkman, Eilis Hannon, Jonathan Mill
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	isoform, transcript, expression, brain, cortex, mouse, human, adult, fetal, long-read sequencing, alternative splicing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1417caefb134428cac84fed4a4f86a78
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spelling	oai:doaj.org-article:1417caefb134428cac84fed4a4f86a782021-11-18T04:47:59ZFull-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing2211-124710.1016/j.celrep.2021.110022https://doaj.org/article/1417caefb134428cac84fed4a4f86a782021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721015047https://doaj.org/toc/2211-1247Summary: Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA molecules from a single pre-mRNA with a prominent role in the development and function of the central nervous system. We used long-read isoform sequencing to generate full-length transcript sequences in the human and mouse cortex. We identify novel transcripts not present in existing genome annotations, including transcripts mapping to putative novel (unannotated) genes and fusion transcripts incorporating exons from multiple genes. Global patterns of transcript diversity are similar between human and mouse cortex, although certain genes are characterized by striking differences between species. We also identify developmental changes in alternative splicing, with differential transcript usage between human fetal and adult cortex. Our data confirm the importance of alternative splicing in the cortex, dramatically increasing transcriptional diversity and representing an important mechanism underpinning gene regulation in the brain. We provide transcript-level data for human and mouse cortex as a resource to the scientific community.Szi Kay LeungAaron R. JeffriesIsabel CastanhoBen T. JordanKaren MooreJonathan P. DaviesEmma L. DempsterNicholas J. BrayPaul O’NeillElizabeth TsengZeshan AhmedDavid A. CollierErin D. JefferyShyam PrabhakarLeonard SchalkwykConnor JopsMichael J. GandalGloria M. SheynkmanEilis HannonJonathan MillElsevierarticleisoform, transcript, expression, brain, cortex, mouse, human, adult, fetal, long-read sequencing, alternative splicingBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 7, Pp 110022- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	isoform, transcript, expression, brain, cortex, mouse, human, adult, fetal, long-read sequencing, alternative splicing Biology (General) QH301-705.5
spellingShingle	isoform, transcript, expression, brain, cortex, mouse, human, adult, fetal, long-read sequencing, alternative splicing Biology (General) QH301-705.5 Szi Kay Leung Aaron R. Jeffries Isabel Castanho Ben T. Jordan Karen Moore Jonathan P. Davies Emma L. Dempster Nicholas J. Bray Paul O’Neill Elizabeth Tseng Zeshan Ahmed David A. Collier Erin D. Jeffery Shyam Prabhakar Leonard Schalkwyk Connor Jops Michael J. Gandal Gloria M. Sheynkman Eilis Hannon Jonathan Mill Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
description	Summary: Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA molecules from a single pre-mRNA with a prominent role in the development and function of the central nervous system. We used long-read isoform sequencing to generate full-length transcript sequences in the human and mouse cortex. We identify novel transcripts not present in existing genome annotations, including transcripts mapping to putative novel (unannotated) genes and fusion transcripts incorporating exons from multiple genes. Global patterns of transcript diversity are similar between human and mouse cortex, although certain genes are characterized by striking differences between species. We also identify developmental changes in alternative splicing, with differential transcript usage between human fetal and adult cortex. Our data confirm the importance of alternative splicing in the cortex, dramatically increasing transcriptional diversity and representing an important mechanism underpinning gene regulation in the brain. We provide transcript-level data for human and mouse cortex as a resource to the scientific community.
format	article
author	Szi Kay Leung Aaron R. Jeffries Isabel Castanho Ben T. Jordan Karen Moore Jonathan P. Davies Emma L. Dempster Nicholas J. Bray Paul O’Neill Elizabeth Tseng Zeshan Ahmed David A. Collier Erin D. Jeffery Shyam Prabhakar Leonard Schalkwyk Connor Jops Michael J. Gandal Gloria M. Sheynkman Eilis Hannon Jonathan Mill
author_facet	Szi Kay Leung Aaron R. Jeffries Isabel Castanho Ben T. Jordan Karen Moore Jonathan P. Davies Emma L. Dempster Nicholas J. Bray Paul O’Neill Elizabeth Tseng Zeshan Ahmed David A. Collier Erin D. Jeffery Shyam Prabhakar Leonard Schalkwyk Connor Jops Michael J. Gandal Gloria M. Sheynkman Eilis Hannon Jonathan Mill
author_sort	Szi Kay Leung
title	Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
title_short	Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
title_full	Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
title_fullStr	Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
title_full_unstemmed	Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
title_sort	full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1417caefb134428cac84fed4a4f86a78
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Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing

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