Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
Wei-Ping Xiao,1,* Li-Li-Qiang Ding,2,* You-Jiang Min,1,3 Hua-Yuan Yang,4 Hai-Hua Yao,3 Jie Sun,1 Xuan Zhou,1 Xue-Bo Zeng,1 Wan Yu1 1Spinal Department of Orthopedics and Department of Acupuncture, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People&...
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2019
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oai:doaj.org-article:1418c0310a1642499e78b7cbe538061a2021-12-02T10:40:30ZElectroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway1178-2021https://doaj.org/article/1418c0310a1642499e78b7cbe538061a2019-12-01T00:00:00Zhttps://www.dovepress.com/electroacupuncture-promoting-axonal-regeneration-in-spinal-cord-injury-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Wei-Ping Xiao,1,* Li-Li-Qiang Ding,2,* You-Jiang Min,1,3 Hua-Yuan Yang,4 Hai-Hua Yao,3 Jie Sun,1 Xuan Zhou,1 Xue-Bo Zeng,1 Wan Yu1 1Spinal Department of Orthopedics and Department of Acupuncture, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People’s Republic of China; 2Department of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Traditional Chinese Medicine, Shanghai Eighth People’s Hospital, Shanghai, People’s Republic of China; 4Institute of Traditional Chinese Medicine Engineering, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: You-Jiang MinShanghai Eighth People’s Hospital, Caobao Road 8, Shanghai 200235, People’s Republic of ChinaTel +86 18221786381Email myj2002@126.com Hua-Yuan YangShanghai University of Traditional Chinese Medicine, Cairen Road 1200, Shanghai 201203, People’s Republic of ChinaTel +86 13651968830Email yhyabcd@sina.comPurpose: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI.Methods: Allen’s method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods.Results: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632treatment alone (P < 0.01 or <0.05). Conclusion: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.Keywords: Nogo/NgR, Rho/ROCK, MLCP, MYPT1, spinal cord injury, Y27632, electroacupunctureXiao WPDing LLQMin YJYang HYYao HHSun JZhou XZeng XBYu WDove Medical Pressarticlenogo/ngrrho/rockmlcpmypt1spinal cord injuryy27632electroacupunctureNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 3429-3442 (2019) |
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DOAJ |
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nogo/ngr rho/rock mlcp mypt1 spinal cord injury y27632 electroacupuncture Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 |
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nogo/ngr rho/rock mlcp mypt1 spinal cord injury y27632 electroacupuncture Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Xiao WP Ding LLQ Min YJ Yang HY Yao HH Sun J Zhou X Zeng XB Yu W Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| description |
Wei-Ping Xiao,1,* Li-Li-Qiang Ding,2,* You-Jiang Min,1,3 Hua-Yuan Yang,4 Hai-Hua Yao,3 Jie Sun,1 Xuan Zhou,1 Xue-Bo Zeng,1 Wan Yu1 1Spinal Department of Orthopedics and Department of Acupuncture, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People’s Republic of China; 2Department of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Traditional Chinese Medicine, Shanghai Eighth People’s Hospital, Shanghai, People’s Republic of China; 4Institute of Traditional Chinese Medicine Engineering, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: You-Jiang MinShanghai Eighth People’s Hospital, Caobao Road 8, Shanghai 200235, People’s Republic of ChinaTel +86 18221786381Email myj2002@126.com
Hua-Yuan YangShanghai University of Traditional Chinese Medicine, Cairen Road 1200, Shanghai 201203, People’s Republic of ChinaTel +86 13651968830Email yhyabcd@sina.comPurpose: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI.Methods: Allen’s method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods.Results: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632treatment alone (P < 0.01 or <0.05).
Conclusion: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.Keywords: Nogo/NgR, Rho/ROCK, MLCP, MYPT1, spinal cord injury, Y27632, electroacupuncture |
| format |
article |
| author |
Xiao WP Ding LLQ Min YJ Yang HY Yao HH Sun J Zhou X Zeng XB Yu W |
| author_facet |
Xiao WP Ding LLQ Min YJ Yang HY Yao HH Sun J Zhou X Zeng XB Yu W |
| author_sort |
Xiao WP |
| title |
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| title_short |
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| title_full |
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| title_fullStr |
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| title_full_unstemmed |
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway |
| title_sort |
electroacupuncture promoting axonal regeneration in spinal cord injury rats via suppression of nogo/ngr and rho/rock signaling pathway |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/1418c0310a1642499e78b7cbe538061a |
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