Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection

This study was conducted to assess the effect of anti-immunoglobulin antibodies onthe measurement of the humoral immune response in hepatitis A virus (HAV) infectedpatients. Serum samples from 47 patients with acute hepatitis A and from 47 age/sexmatched healthy adult subjects were tested for IgA, I...

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Autores principales:	Medhat Haroun, Mohamed M. El-Sayed
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2007
Materias:	Hepatitis A virus Antibodies IgA IgM IgG Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/14191131dcc847b0b916254bc9d72c7e
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spelling	oai:doaj.org-article:14191131dcc847b0b916254bc9d72c7e2021-12-02T13:45:32ZDiagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection0250-46851303-829Xhttps://doaj.org/article/14191131dcc847b0b916254bc9d72c7e2007-05-01T00:00:00Zhttp://www.turkjbiochem.com/2007/022.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XThis study was conducted to assess the effect of anti-immunoglobulin antibodies onthe measurement of the humoral immune response in hepatitis A virus (HAV) infectedpatients. Serum samples from 47 patients with acute hepatitis A and from 47 age/sexmatched healthy adult subjects were tested for IgA, IgG and IgM by ELISA. Antiimmunoglobulinantibodies were defined using goat immunoglobulins as a target tocharacterize distinct changes in levels of interacting immunoglobulins. Initial resultsobtained before removal of antibodies that interacted with goat immunoglobulinssuggested that HAV patients had increased levels of IgA and IgM in their sera. It wasfound that normal individuals had mean IgA, IgG and IgM levels of 2.52 ± 0.22 mg/ml,9.86 ± 0.94 mg/ml and 1.75 ± 0.13 mg/ml, respectively while HAV patients had meanlevels of 2.89 ± 0.20 mg/ml, 10.03 ± 1.05 mg/ml and 1.97 ± 0.13 mg/ml (p <0.0002, p<0.41 and p <0.0001). However, the mean levels of IgA and IgM in HAV infected sera,after purification from antibodies that interacted with goat immunoglobulins, were 2.49± 0.21 mg/ml and 1.73 ± 0.14 mg/ml. Therefore, there was no significant difference inHAV patients compared to normal individuals (p <0.55 and p <0.56). The presence ofcirculating immune complex in serum during the early phase of infection may contributeto immunopathological effects in the infected host and provide some new insights intoantibody response to HAVMedhat Haroun,Mohamed M. El-SayedDe GruyterarticleHepatitis A virusAntibodiesIgAIgMIgGBiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 32, Iss 1, Pp 22-27 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatitis A virus Antibodies IgA IgM IgG Biochemistry QD415-436
spellingShingle	Hepatitis A virus Antibodies IgA IgM IgG Biochemistry QD415-436 Medhat Haroun, Mohamed M. El-Sayed Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
description	This study was conducted to assess the effect of anti-immunoglobulin antibodies onthe measurement of the humoral immune response in hepatitis A virus (HAV) infectedpatients. Serum samples from 47 patients with acute hepatitis A and from 47 age/sexmatched healthy adult subjects were tested for IgA, IgG and IgM by ELISA. Antiimmunoglobulinantibodies were defined using goat immunoglobulins as a target tocharacterize distinct changes in levels of interacting immunoglobulins. Initial resultsobtained before removal of antibodies that interacted with goat immunoglobulinssuggested that HAV patients had increased levels of IgA and IgM in their sera. It wasfound that normal individuals had mean IgA, IgG and IgM levels of 2.52 ± 0.22 mg/ml,9.86 ± 0.94 mg/ml and 1.75 ± 0.13 mg/ml, respectively while HAV patients had meanlevels of 2.89 ± 0.20 mg/ml, 10.03 ± 1.05 mg/ml and 1.97 ± 0.13 mg/ml (p <0.0002, p<0.41 and p <0.0001). However, the mean levels of IgA and IgM in HAV infected sera,after purification from antibodies that interacted with goat immunoglobulins, were 2.49± 0.21 mg/ml and 1.73 ± 0.14 mg/ml. Therefore, there was no significant difference inHAV patients compared to normal individuals (p <0.55 and p <0.56). The presence ofcirculating immune complex in serum during the early phase of infection may contributeto immunopathological effects in the infected host and provide some new insights intoantibody response to HAV
format	article
author	Medhat Haroun, Mohamed M. El-Sayed
author_facet	Medhat Haroun, Mohamed M. El-Sayed
author_sort	Medhat Haroun,
title	Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
title_short	Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
title_full	Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
title_fullStr	Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
title_full_unstemmed	Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection
title_sort	diagnostic value of immunoglobulin a and m antibodies as a disease marker for hepatitis a infection
publisher	De Gruyter
publishDate	2007
url	https://doaj.org/article/14191131dcc847b0b916254bc9d72c7e
work_keys_str_mv	AT medhatharoun diagnosticvalueofimmunoglobulinaandmantibodiesasadiseasemarkerforhepatitisainfection AT mohamedmelsayed diagnosticvalueofimmunoglobulinaandmantibodiesasadiseasemarkerforhepatitisainfection
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Diagnostic Value of Immunoglobulin A and M antibodies as a disease Marker for hepatitis A Infection

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