A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species

Abstract The genome of Fusarium oxysporum (Fo) consists of a set of eleven ‘core’ chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. We sequenced a strain of Fo f.sp. radicis-cucumerinum (Forc) using PacBio SMRT sequencing. All but one of...

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Autores principales:	Peter van Dam, Like Fokkens, Yu Ayukawa, Michelle van der Gragt, Anneliek ter Horst, Balázs Brankovics, Petra M. Houterman, Tsutomu Arie, Martijn Rep
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/141f1e23397942e99924c4b0e9aa3434
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spelling	oai:doaj.org-article:141f1e23397942e99924c4b0e9aa34342021-12-02T16:06:50ZA mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species10.1038/s41598-017-07995-y2045-2322https://doaj.org/article/141f1e23397942e99924c4b0e9aa34342017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07995-yhttps://doaj.org/toc/2045-2322Abstract The genome of Fusarium oxysporum (Fo) consists of a set of eleven ‘core’ chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. We sequenced a strain of Fo f.sp. radicis-cucumerinum (Forc) using PacBio SMRT sequencing. All but one of the core chromosomes were assembled into single contigs, and a chromosome that shows all the hallmarks of a pathogenicity chromosome comprised two contigs. A central part of this chromosome contains all identified candidate effector genes, including homologs of SIX6, SIX9, SIX11 and SIX 13. We show that SIX6 contributes to virulence of Forc. Through horizontal chromosome transfer (HCT) to a non-pathogenic strain, we also show that the accessory chromosome containing the SIX gene homologs is indeed a pathogenicity chromosome for cucurbit infection. Conversely, complete loss of virulence was observed in Forc016 strains that lost this chromosome. We conclude that also a non-wilt-inducing Fo pathogen relies on effector proteins for successful infection and that the Forc pathogenicity chromosome contains all the information necessary for causing root rot of cucurbits. Three out of nine HCT strains investigated have undergone large-scale chromosome alterations, reflecting the remarkable plasticity of Fo genomes.Peter van DamLike FokkensYu AyukawaMichelle van der GragtAnneliek ter HorstBalázs BrankovicsPetra M. HoutermanTsutomu ArieMartijn RepNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter van Dam Like Fokkens Yu Ayukawa Michelle van der Gragt Anneliek ter Horst Balázs Brankovics Petra M. Houterman Tsutomu Arie Martijn Rep A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
description	Abstract The genome of Fusarium oxysporum (Fo) consists of a set of eleven ‘core’ chromosomes, shared by most strains and responsible for housekeeping, and one or several accessory chromosomes. We sequenced a strain of Fo f.sp. radicis-cucumerinum (Forc) using PacBio SMRT sequencing. All but one of the core chromosomes were assembled into single contigs, and a chromosome that shows all the hallmarks of a pathogenicity chromosome comprised two contigs. A central part of this chromosome contains all identified candidate effector genes, including homologs of SIX6, SIX9, SIX11 and SIX 13. We show that SIX6 contributes to virulence of Forc. Through horizontal chromosome transfer (HCT) to a non-pathogenic strain, we also show that the accessory chromosome containing the SIX gene homologs is indeed a pathogenicity chromosome for cucurbit infection. Conversely, complete loss of virulence was observed in Forc016 strains that lost this chromosome. We conclude that also a non-wilt-inducing Fo pathogen relies on effector proteins for successful infection and that the Forc pathogenicity chromosome contains all the information necessary for causing root rot of cucurbits. Three out of nine HCT strains investigated have undergone large-scale chromosome alterations, reflecting the remarkable plasticity of Fo genomes.
format	article
author	Peter van Dam Like Fokkens Yu Ayukawa Michelle van der Gragt Anneliek ter Horst Balázs Brankovics Petra M. Houterman Tsutomu Arie Martijn Rep
author_facet	Peter van Dam Like Fokkens Yu Ayukawa Michelle van der Gragt Anneliek ter Horst Balázs Brankovics Petra M. Houterman Tsutomu Arie Martijn Rep
author_sort	Peter van Dam
title	A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
title_short	A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
title_full	A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
title_fullStr	A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
title_full_unstemmed	A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species
title_sort	mobile pathogenicity chromosome in fusarium oxysporum for infection of multiple cucurbit species
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/141f1e23397942e99924c4b0e9aa3434
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A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species

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