Mueller matrix imaging for collagen scoring in mice model of pregnancy

Abstract Preterm birth risk is associated with early softening of the uterine cervix in pregnancy due to the accelerated remodeling of collagen extracellular matrix. Studies of mice model of pregnancy were performed with an imaging Mueller polarimeter at different time points of pregnancy to find po...

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Autores principales:	Hee Ryung Lee, Ilyas Saytashev, Vinh Nguyen Du Le, Mala Mahendroo, Jessica Ramella-Roman, Tatiana Novikova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/142d02fb78754b32994e37742b383e63
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spelling	oai:doaj.org-article:142d02fb78754b32994e37742b383e632021-12-02T16:35:19ZMueller matrix imaging for collagen scoring in mice model of pregnancy10.1038/s41598-021-95020-82045-2322https://doaj.org/article/142d02fb78754b32994e37742b383e632021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95020-8https://doaj.org/toc/2045-2322Abstract Preterm birth risk is associated with early softening of the uterine cervix in pregnancy due to the accelerated remodeling of collagen extracellular matrix. Studies of mice model of pregnancy were performed with an imaging Mueller polarimeter at different time points of pregnancy to find polarimetric parameters for collagen scoring. Mueller matrix images of the unstained sections of mice uterine cervices were taken at day 6 and day 18 of 19-days gestation period and at different spatial locations through the cervices. The logarithmic decomposition of the recorded Mueller matrices mapped the depolarization, linear retardance, and azimuth of the optical axis of cervical tissue. These images highlighted both the inner structure of cervix and the arrangement of cervical collagen fibers confirmed by the second harmonic generation microscopy. The statistical analysis and two-Gaussians fit of the distributions of linear retardance and linear depolarization in the entire images of cervical tissue (without manual selection of the specific regions of interest) quantified the randomization of collagen fibers alignment with gestation time. At day 18 the remodeling of cervical extracellular matrix of collagen was measurable at the external cervical os that is available for the direct optical observations in vivo. It supports the assumption that imaging Mueller polarimetry holds promise for the fast and accurate collagen scoring in pregnancy and the assessment of the preterm birth risk.Hee Ryung LeeIlyas SaytashevVinh Nguyen Du LeMala MahendrooJessica Ramella-RomanTatiana NovikovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hee Ryung Lee Ilyas Saytashev Vinh Nguyen Du Le Mala Mahendroo Jessica Ramella-Roman Tatiana Novikova Mueller matrix imaging for collagen scoring in mice model of pregnancy
description	Abstract Preterm birth risk is associated with early softening of the uterine cervix in pregnancy due to the accelerated remodeling of collagen extracellular matrix. Studies of mice model of pregnancy were performed with an imaging Mueller polarimeter at different time points of pregnancy to find polarimetric parameters for collagen scoring. Mueller matrix images of the unstained sections of mice uterine cervices were taken at day 6 and day 18 of 19-days gestation period and at different spatial locations through the cervices. The logarithmic decomposition of the recorded Mueller matrices mapped the depolarization, linear retardance, and azimuth of the optical axis of cervical tissue. These images highlighted both the inner structure of cervix and the arrangement of cervical collagen fibers confirmed by the second harmonic generation microscopy. The statistical analysis and two-Gaussians fit of the distributions of linear retardance and linear depolarization in the entire images of cervical tissue (without manual selection of the specific regions of interest) quantified the randomization of collagen fibers alignment with gestation time. At day 18 the remodeling of cervical extracellular matrix of collagen was measurable at the external cervical os that is available for the direct optical observations in vivo. It supports the assumption that imaging Mueller polarimetry holds promise for the fast and accurate collagen scoring in pregnancy and the assessment of the preterm birth risk.
format	article
author	Hee Ryung Lee Ilyas Saytashev Vinh Nguyen Du Le Mala Mahendroo Jessica Ramella-Roman Tatiana Novikova
author_facet	Hee Ryung Lee Ilyas Saytashev Vinh Nguyen Du Le Mala Mahendroo Jessica Ramella-Roman Tatiana Novikova
author_sort	Hee Ryung Lee
title	Mueller matrix imaging for collagen scoring in mice model of pregnancy
title_short	Mueller matrix imaging for collagen scoring in mice model of pregnancy
title_full	Mueller matrix imaging for collagen scoring in mice model of pregnancy
title_fullStr	Mueller matrix imaging for collagen scoring in mice model of pregnancy
title_full_unstemmed	Mueller matrix imaging for collagen scoring in mice model of pregnancy
title_sort	mueller matrix imaging for collagen scoring in mice model of pregnancy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/142d02fb78754b32994e37742b383e63
work_keys_str_mv	AT heeryunglee muellermatriximagingforcollagenscoringinmicemodelofpregnancy AT ilyassaytashev muellermatriximagingforcollagenscoringinmicemodelofpregnancy AT vinhnguyendule muellermatriximagingforcollagenscoringinmicemodelofpregnancy AT malamahendroo muellermatriximagingforcollagenscoringinmicemodelofpregnancy AT jessicaramellaroman muellermatriximagingforcollagenscoringinmicemodelofpregnancy AT tatiananovikova muellermatriximagingforcollagenscoringinmicemodelofpregnancy
_version_	1718383677494263808

Mueller matrix imaging for collagen scoring in mice model of pregnancy

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