circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells

Oxidized low-density lipoprotein (ox-LDL) is a significant risk factor for various brain vascular diseases. Circular RNA (circRNA) is involved in the pathogenesis of brain vascular diseases. This study revealed the roles of circ_CHFR in ox-LDL-mediated cell proliferation, apoptosis, and endothelial-...

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Autores principales:	Wu Shanwu, Yang Sheng, Qu Hongyan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	ox-ldl circ_chfr mir-15a-5p egfr hbmecs Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/142f1801e85347388026ae910b118dec
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spelling	oai:doaj.org-article:142f1801e85347388026ae910b118dec2021-12-05T14:10:41Zcirc_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells2391-541210.1515/biol-2021-0082https://doaj.org/article/142f1801e85347388026ae910b118dec2021-09-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0082https://doaj.org/toc/2391-5412Oxidized low-density lipoprotein (ox-LDL) is a significant risk factor for various brain vascular diseases. Circular RNA (circRNA) is involved in the pathogenesis of brain vascular diseases. This study revealed the roles of circ_CHFR in ox-LDL-mediated cell proliferation, apoptosis, and endothelial-to-mesenchymal transition (EndoMT). Our results showed that circ_CHFR and EGFR expressions were dramatically upregulated, while miR-15a-5p expression was downregulated in ox-LDL-induced human brain microvessel endothelial cells (HBMECs) relative to control groups. circ_CHFR knockdown hindered the effects of ox-LDL exposure on cell proliferation, cell cycle, apoptosis, and EndoMT in HBMECs, whereas these impacts were abolished by miR-15a-5p inhibitor. In addition, circ_CHFR functioned as a sponge of miR-15a-5p and miR-15a-5p bound to EGFR. Thus, we concluded that circ_CHFR silencing hindered ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by downregulating EGFR expression through sponging miR-15a-5p in HBMECs. Our findings provide a new mechanism for studying circRNA-directed therapy in ox-LDL-induced human brain vascular diseases.Wu ShanwuYang ShengQu HongyanDe Gruyterarticleox-ldlcirc_chfrmir-15a-5pegfrhbmecsBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1053-1063 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ox-ldl circ_chfr mir-15a-5p egfr hbmecs Biology (General) QH301-705.5
spellingShingle	ox-ldl circ_chfr mir-15a-5p egfr hbmecs Biology (General) QH301-705.5 Wu Shanwu Yang Sheng Qu Hongyan circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
description	Oxidized low-density lipoprotein (ox-LDL) is a significant risk factor for various brain vascular diseases. Circular RNA (circRNA) is involved in the pathogenesis of brain vascular diseases. This study revealed the roles of circ_CHFR in ox-LDL-mediated cell proliferation, apoptosis, and endothelial-to-mesenchymal transition (EndoMT). Our results showed that circ_CHFR and EGFR expressions were dramatically upregulated, while miR-15a-5p expression was downregulated in ox-LDL-induced human brain microvessel endothelial cells (HBMECs) relative to control groups. circ_CHFR knockdown hindered the effects of ox-LDL exposure on cell proliferation, cell cycle, apoptosis, and EndoMT in HBMECs, whereas these impacts were abolished by miR-15a-5p inhibitor. In addition, circ_CHFR functioned as a sponge of miR-15a-5p and miR-15a-5p bound to EGFR. Thus, we concluded that circ_CHFR silencing hindered ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by downregulating EGFR expression through sponging miR-15a-5p in HBMECs. Our findings provide a new mechanism for studying circRNA-directed therapy in ox-LDL-induced human brain vascular diseases.
format	article
author	Wu Shanwu Yang Sheng Qu Hongyan
author_facet	Wu Shanwu Yang Sheng Qu Hongyan
author_sort	Wu Shanwu
title	circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
title_short	circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
title_full	circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
title_fullStr	circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
title_full_unstemmed	circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells
title_sort	circ_chfr regulates ox-ldl-mediated cell proliferation, apoptosis, and endomt by mir-15a-5p/egfr axis in human brain microvessel endothelial cells
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/142f1801e85347388026ae910b118dec
work_keys_str_mv	AT wushanwu circchfrregulatesoxldlmediatedcellproliferationapoptosisandendomtbymir15a5pegfraxisinhumanbrainmicrovesselendothelialcells AT yangsheng circchfrregulatesoxldlmediatedcellproliferationapoptosisandendomtbymir15a5pegfraxisinhumanbrainmicrovesselendothelialcells AT quhongyan circchfrregulatesoxldlmediatedcellproliferationapoptosisandendomtbymir15a5pegfraxisinhumanbrainmicrovesselendothelialcells
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circ_CHFR regulates ox-LDL-mediated cell proliferation, apoptosis, and EndoMT by miR-15a-5p/EGFR axis in human brain microvessel endothelial cells

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