Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway

Although several treatments are available for the treatment of type 2 diabetes mellitus, adverse effects and cost burden impose the search for safe, efficient, and cost-effective alternative herbal remedies. Syzygium aqueum (Burm.f.) Alston, a natural anti-inflammatory, antioxidant herb, may suppres...

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Autores principales:	Mona F. Mahmoud, Shimaa Abdelaal, Heba Osama Mohammed, Assem M. El-Shazly, Rachid Daoud, Mohamed A. O. Abdelfattah, Mansour Sobeh
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Syzygium aqueum TLR-4 TRAF-6 MyD88 HO-1 diabetes Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/1438a8bf00aa4ba798f8035a799f9263
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spelling	oai:doaj.org-article:1438a8bf00aa4ba798f8035a799f92632021-12-01T13:36:06ZSyzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway1663-981210.3389/fphar.2021.769244https://doaj.org/article/1438a8bf00aa4ba798f8035a799f92632021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.769244/fullhttps://doaj.org/toc/1663-9812Although several treatments are available for the treatment of type 2 diabetes mellitus, adverse effects and cost burden impose the search for safe, efficient, and cost-effective alternative herbal remedies. Syzygium aqueum (Burm.f.) Alston, a natural anti-inflammatory, antioxidant herb, may suppress diabetes-associated inflammation and pancreatic beta-cell death. Here, we tested the ability of the bioactive leaf extract (SA) to prevent streptozotocin (STZ)-induced oxidative stress and inflammation in pancreatic beta cells in rats and the involvement of the TLR-4 signaling pathway. Non-fasted rats pretreated with 100 or 200 mg kg−1 SA 2 days prior to the STZ challenge and for 14 days later had up to 52 and 39% reduction in the glucose levels, respectively, while glibenclamide, the reference standard drug (0.5 mg kg−1), results in 70% reduction. Treatment with SA extract was accompanied by increased insulin secretion, restoration of Langerhans islets morphology, and decreased collagen deposition as demonstrated from ELISA measurement, H and E, and Mallory staining. Both glibenclamide and SA extract significantly decreased levels of TLR-4, MYD88, pro-inflammatory cytokines TNF-α, and TRAF-6 in pancreatic tissue homogenates, which correlated well with minimal pancreatic inflammatory cell infiltration. Pre-treatment with SA or glibenclamide decreased malondialdehyde, a sensitive biomarker of ROS-induced lipid peroxidation, and restored depleted reduced glutathione in the pancreas. Altogether, these data indicate that S. aqueum is effective in improving STZ-induced pancreatic damage, which could be beneficial in treating type 2 diabetes mellitus.Mona F. MahmoudShimaa AbdelaalHeba Osama MohammedAssem M. El-ShazlyRachid DaoudMohamed A. O. AbdelfattahMansour SobehFrontiers Media S.A.articleSyzygium aqueumTLR-4TRAF-6MyD88HO-1diabetesTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Syzygium aqueum TLR-4 TRAF-6 MyD88 HO-1 diabetes Therapeutics. Pharmacology RM1-950
spellingShingle	Syzygium aqueum TLR-4 TRAF-6 MyD88 HO-1 diabetes Therapeutics. Pharmacology RM1-950 Mona F. Mahmoud Shimaa Abdelaal Heba Osama Mohammed Assem M. El-Shazly Rachid Daoud Mohamed A. O. Abdelfattah Mansour Sobeh Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
description	Although several treatments are available for the treatment of type 2 diabetes mellitus, adverse effects and cost burden impose the search for safe, efficient, and cost-effective alternative herbal remedies. Syzygium aqueum (Burm.f.) Alston, a natural anti-inflammatory, antioxidant herb, may suppress diabetes-associated inflammation and pancreatic beta-cell death. Here, we tested the ability of the bioactive leaf extract (SA) to prevent streptozotocin (STZ)-induced oxidative stress and inflammation in pancreatic beta cells in rats and the involvement of the TLR-4 signaling pathway. Non-fasted rats pretreated with 100 or 200 mg kg−1 SA 2 days prior to the STZ challenge and for 14 days later had up to 52 and 39% reduction in the glucose levels, respectively, while glibenclamide, the reference standard drug (0.5 mg kg−1), results in 70% reduction. Treatment with SA extract was accompanied by increased insulin secretion, restoration of Langerhans islets morphology, and decreased collagen deposition as demonstrated from ELISA measurement, H and E, and Mallory staining. Both glibenclamide and SA extract significantly decreased levels of TLR-4, MYD88, pro-inflammatory cytokines TNF-α, and TRAF-6 in pancreatic tissue homogenates, which correlated well with minimal pancreatic inflammatory cell infiltration. Pre-treatment with SA or glibenclamide decreased malondialdehyde, a sensitive biomarker of ROS-induced lipid peroxidation, and restored depleted reduced glutathione in the pancreas. Altogether, these data indicate that S. aqueum is effective in improving STZ-induced pancreatic damage, which could be beneficial in treating type 2 diabetes mellitus.
format	article
author	Mona F. Mahmoud Shimaa Abdelaal Heba Osama Mohammed Assem M. El-Shazly Rachid Daoud Mohamed A. O. Abdelfattah Mansour Sobeh
author_facet	Mona F. Mahmoud Shimaa Abdelaal Heba Osama Mohammed Assem M. El-Shazly Rachid Daoud Mohamed A. O. Abdelfattah Mansour Sobeh
author_sort	Mona F. Mahmoud
title	Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
title_short	Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
title_full	Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
title_fullStr	Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
title_full_unstemmed	Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway
title_sort	syzygium aqueum (burm.f.) alston prevents streptozotocin-induced pancreatic beta cells damage via the tlr-4 signaling pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1438a8bf00aa4ba798f8035a799f9263
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Syzygium aqueum (Burm.f.) Alston Prevents Streptozotocin-Induced Pancreatic Beta Cells Damage via the TLR-4 Signaling Pathway

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