In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period

Grafting of microencapsulated pancreatic islets has been proposed as an alternative to exogenous insulin for the treatment of type 1 diabetes mellitus. Microencapsulated islets are protected from direct contact with immune cells and larger immune‐active molecules such as immunoglobulins. Unfortunate...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carlos E. Navarro Chica, Tian Qin, Bart J. de Haan, Marijke M. Faas, Alexandra M. Smink, Ligia Sierra, Betty L. López, Paul de Vos
Formato:	article
Lenguaje:	EN
Publicado:	Wiley-VCH 2021
Materias:	alginate microcapsules gusperimus controlled release islet microencapsulation local immunosuppression squalene-gusperimus nanoparticles type 1 diabetes mellitus Biotechnology TP248.13-248.65 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/1449a3cc0a6d4c959af56e0e02c79114
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1449a3cc0a6d4c959af56e0e02c79114
record_format	dspace
spelling	oai:doaj.org-article:1449a3cc0a6d4c959af56e0e02c791142021-11-06T04:12:26ZIn Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period2699-930710.1002/anbr.202100055https://doaj.org/article/1449a3cc0a6d4c959af56e0e02c791142021-11-01T00:00:00Zhttps://doi.org/10.1002/anbr.202100055https://doaj.org/toc/2699-9307Grafting of microencapsulated pancreatic islets has been proposed as an alternative to exogenous insulin for the treatment of type 1 diabetes mellitus. Microencapsulated islets are protected from direct contact with immune cells and larger immune‐active molecules such as immunoglobulins. Unfortunately, many islet cells in the microcapsules are lost in the immediate period after transplantation due to an early host immune response limiting long‐term function of the graft. Gusperimus has shown to reduce the inflammatory responses to grafted encapsulated islets, but it cannot be appropriately used because it is easily hydrolyzed leading to loss of activity. To temporarily modulate the inflammatory response directly after implantation and stabilize gusperimus, squalene‐gusperimus nanoparticles (Sq‐GusNPs) are developed and incorporated in human islets‐containing alginate‐based microcapsules. A prolonged and continuous release of gusperimus is achieved. This offers an anti‐inflammatory microenvironment in the vicinity of the microcapsules and a reduction of cytokine secretion by lipopolysaccharides‐activated human macrophages. Release of gusperimus from Sq‐GusNPs does not affect the in vitro viability or function of human pancreatic islets. The data illustrate that incorporation of Sq‐GusNPs in alginate microcapsules offers an opportunity to temporarily modulate the immediate immune response after the grafting procedure of encapsulated islets cells and reduce loss of islet cells.Carlos E. Navarro ChicaTian QinBart J. de HaanMarijke M. FaasAlexandra M. SminkLigia SierraBetty L. LópezPaul de VosWiley-VCHarticlealginate microcapsulesgusperimus controlled releaseislet microencapsulationlocal immunosuppressionsqualene-gusperimus nanoparticlestype 1 diabetes mellitusBiotechnologyTP248.13-248.65Medical technologyR855-855.5ENAdvanced NanoBiomed Research, Vol 1, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alginate microcapsules gusperimus controlled release islet microencapsulation local immunosuppression squalene-gusperimus nanoparticles type 1 diabetes mellitus Biotechnology TP248.13-248.65 Medical technology R855-855.5
spellingShingle	alginate microcapsules gusperimus controlled release islet microencapsulation local immunosuppression squalene-gusperimus nanoparticles type 1 diabetes mellitus Biotechnology TP248.13-248.65 Medical technology R855-855.5 Carlos E. Navarro Chica Tian Qin Bart J. de Haan Marijke M. Faas Alexandra M. Smink Ligia Sierra Betty L. López Paul de Vos In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
description	Grafting of microencapsulated pancreatic islets has been proposed as an alternative to exogenous insulin for the treatment of type 1 diabetes mellitus. Microencapsulated islets are protected from direct contact with immune cells and larger immune‐active molecules such as immunoglobulins. Unfortunately, many islet cells in the microcapsules are lost in the immediate period after transplantation due to an early host immune response limiting long‐term function of the graft. Gusperimus has shown to reduce the inflammatory responses to grafted encapsulated islets, but it cannot be appropriately used because it is easily hydrolyzed leading to loss of activity. To temporarily modulate the inflammatory response directly after implantation and stabilize gusperimus, squalene‐gusperimus nanoparticles (Sq‐GusNPs) are developed and incorporated in human islets‐containing alginate‐based microcapsules. A prolonged and continuous release of gusperimus is achieved. This offers an anti‐inflammatory microenvironment in the vicinity of the microcapsules and a reduction of cytokine secretion by lipopolysaccharides‐activated human macrophages. Release of gusperimus from Sq‐GusNPs does not affect the in vitro viability or function of human pancreatic islets. The data illustrate that incorporation of Sq‐GusNPs in alginate microcapsules offers an opportunity to temporarily modulate the immediate immune response after the grafting procedure of encapsulated islets cells and reduce loss of islet cells.
format	article
author	Carlos E. Navarro Chica Tian Qin Bart J. de Haan Marijke M. Faas Alexandra M. Smink Ligia Sierra Betty L. López Paul de Vos
author_facet	Carlos E. Navarro Chica Tian Qin Bart J. de Haan Marijke M. Faas Alexandra M. Smink Ligia Sierra Betty L. López Paul de Vos
author_sort	Carlos E. Navarro Chica
title	In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
title_short	In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
title_full	In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
title_fullStr	In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
title_full_unstemmed	In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period
title_sort	in vitro studies of squalene‐gusperimus nanoparticles in islet‐containing alginate microcapsules to regulate the immune response in the immediate posttransplant period
publisher	Wiley-VCH
publishDate	2021
url	https://doaj.org/article/1449a3cc0a6d4c959af56e0e02c79114
work_keys_str_mv	AT carlosenavarrochica invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT tianqin invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT bartjdehaan invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT marijkemfaas invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT alexandramsmink invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT ligiasierra invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT bettyllopez invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod AT pauldevos invitrostudiesofsqualenegusperimusnanoparticlesinisletcontainingalginatemicrocapsulestoregulatetheimmuneresponseintheimmediateposttransplantperiod
_version_	1718443900041953280

In Vitro Studies of Squalene‐Gusperimus Nanoparticles in Islet‐Containing Alginate Microcapsules to Regulate the Immune Response in the Immediate Posttransplant Period

Ejemplares similares