Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults

Recent findings suggested that proteins can differentially affect carotenoid bioaccessibility during gastro-intestinal digestion. In this crossover, randomized human trial, we aimed to confirm that proteins, specifically whey- and soy-protein isolates (WPI/SPI) impact postprandial carotenoid bioavai...

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Autores principales:	Mohammed Iddir, Denis Pittois, Cédric Guignard, Bernard Weber, Manon Gantenbein, Yvan Larondelle, Torsten Bohn
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carotenoids enzymes micellization plant vs. animal proteins emulsification xanthophylls Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/1453dc9cf3b54b3fae69b5e9106dc7ae
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spelling	oai:doaj.org-article:1453dc9cf3b54b3fae69b5e9106dc7ae2021-11-25T16:27:45ZWhey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults10.3390/antiox101117482076-3921https://doaj.org/article/1453dc9cf3b54b3fae69b5e9106dc7ae2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1748https://doaj.org/toc/2076-3921Recent findings suggested that proteins can differentially affect carotenoid bioaccessibility during gastro-intestinal digestion. In this crossover, randomized human trial, we aimed to confirm that proteins, specifically whey- and soy-protein isolates (WPI/SPI) impact postprandial carotenoid bioavailability. Healthy adults (<i>n</i> = 12 males, <i>n</i> = 12 females) were recruited. After 2-week washout periods, 350 g of a tomato-carrot juice mixture was served in the absence/presence of WPI or SPI (50% of the recommended dietary allowance, RDA ≈ 60 g/d). Absorption kinetics of carotenoids and triacylglycerols (TAGs) were evaluated via the triacylglycerol-rich lipoprotein (TRL) fraction response, at timed intervals up to 10 h after test meal intake, on three occasions separated by 1 week. Maximum TRL-carotenoid concentration (C<sub>max</sub>) and corresponding time (T<sub>max</sub>) were also determined. Considering both genders and carotenoids/TAGs combined, the estimated area under the curve (AUC) for WPI increased by 45% vs. the control (<i>p</i> = 0.018), to 92.0 ± 1.7 nmol × h/L and by 57% vs. SPI (<i>p</i> = 0.006). Test meal effect was significant in males (<i>p</i> = 0.036), but not in females (<i>p</i> = 0.189). In males, significant differences were found for phytoene (<i>p</i> = 0.026), phytofluene (<i>p</i> = 0.004), α-carotene (<i>p</i> = 0.034), and β-carotene (<i>p</i> = 0.031). C<sub>max</sub> for total carotenoids (nmol/L ± SD) was positively influenced by WPI (135.4 ± 38.0), while significantly lowered by SPI (89.6 ± 17.3 nmol/L) vs. the control (119.6 ± 30.9, <i>p</i> < 0.001). T<sub>max</sub> did not change. The results suggest that a well-digestible protein could enhance carotenoid bioavailability, whereas the less digestible SPI results in negative effects. This is, to our knowledge, the first study finding effects of proteins on carotenoid absorption in humans.Mohammed IddirDenis PittoisCédric GuignardBernard WeberManon GantenbeinYvan LarondelleTorsten BohnMDPI AGarticlecarotenoidsenzymesmicellizationplant vs. animal proteinsemulsificationxanthophyllsTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1748, p 1748 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carotenoids enzymes micellization plant vs. animal proteins emulsification xanthophylls Therapeutics. Pharmacology RM1-950
spellingShingle	carotenoids enzymes micellization plant vs. animal proteins emulsification xanthophylls Therapeutics. Pharmacology RM1-950 Mohammed Iddir Denis Pittois Cédric Guignard Bernard Weber Manon Gantenbein Yvan Larondelle Torsten Bohn Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
description	Recent findings suggested that proteins can differentially affect carotenoid bioaccessibility during gastro-intestinal digestion. In this crossover, randomized human trial, we aimed to confirm that proteins, specifically whey- and soy-protein isolates (WPI/SPI) impact postprandial carotenoid bioavailability. Healthy adults (<i>n</i> = 12 males, <i>n</i> = 12 females) were recruited. After 2-week washout periods, 350 g of a tomato-carrot juice mixture was served in the absence/presence of WPI or SPI (50% of the recommended dietary allowance, RDA ≈ 60 g/d). Absorption kinetics of carotenoids and triacylglycerols (TAGs) were evaluated via the triacylglycerol-rich lipoprotein (TRL) fraction response, at timed intervals up to 10 h after test meal intake, on three occasions separated by 1 week. Maximum TRL-carotenoid concentration (C<sub>max</sub>) and corresponding time (T<sub>max</sub>) were also determined. Considering both genders and carotenoids/TAGs combined, the estimated area under the curve (AUC) for WPI increased by 45% vs. the control (<i>p</i> = 0.018), to 92.0 ± 1.7 nmol × h/L and by 57% vs. SPI (<i>p</i> = 0.006). Test meal effect was significant in males (<i>p</i> = 0.036), but not in females (<i>p</i> = 0.189). In males, significant differences were found for phytoene (<i>p</i> = 0.026), phytofluene (<i>p</i> = 0.004), α-carotene (<i>p</i> = 0.034), and β-carotene (<i>p</i> = 0.031). C<sub>max</sub> for total carotenoids (nmol/L ± SD) was positively influenced by WPI (135.4 ± 38.0), while significantly lowered by SPI (89.6 ± 17.3 nmol/L) vs. the control (119.6 ± 30.9, <i>p</i> < 0.001). T<sub>max</sub> did not change. The results suggest that a well-digestible protein could enhance carotenoid bioavailability, whereas the less digestible SPI results in negative effects. This is, to our knowledge, the first study finding effects of proteins on carotenoid absorption in humans.
format	article
author	Mohammed Iddir Denis Pittois Cédric Guignard Bernard Weber Manon Gantenbein Yvan Larondelle Torsten Bohn
author_facet	Mohammed Iddir Denis Pittois Cédric Guignard Bernard Weber Manon Gantenbein Yvan Larondelle Torsten Bohn
author_sort	Mohammed Iddir
title	Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
title_short	Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
title_full	Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
title_fullStr	Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
title_full_unstemmed	Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults
title_sort	whey- and soy protein isolates added to a carrot-tomato juice alter carotenoid bioavailability in healthy adults
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1453dc9cf3b54b3fae69b5e9106dc7ae
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Whey- and Soy Protein Isolates Added to a Carrot-Tomato Juice Alter Carotenoid Bioavailability in Healthy Adults

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