Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia

Hasmiwati, Supargiyono. 2018. Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia. Biodiversitas 19: 552-558. Aedes aegypti, the primary vector of Dengue fever, is widely distributed in Indonesia including in West Sumatra. Che...

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Autores principales:	HASMIWATI HASMIWATI, SUPARGIYONO SUPARGIYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	aedes aegypti resistance primer design as-pcr kdr mutation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/145d62fd6c7842ada99e53c72f7d9bd0
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spelling	oai:doaj.org-article:145d62fd6c7842ada99e53c72f7d9bd02021-11-16T14:01:41ZShort Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia1412-033X2085-472210.13057/biodiv/d190225https://doaj.org/article/145d62fd6c7842ada99e53c72f7d9bd02018-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2520https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hasmiwati, Supargiyono. 2018. Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia. Biodiversitas 19: 552-558. Aedes aegypti, the primary vector of Dengue fever, is widely distributed in Indonesia including in West Sumatra. Chemical insecticide is an effective way of shedding the chain of Dengue fever transmission. Long-term applications of insecticides have resulted in the development of resistance in Ae. aegypti populations. Knockdown resistance (kdr) allele as a result of point mutations has been found in Voltage-Gated Sodium Channel (VGSC) gene. This study aimed to design allele-specific primers to detect the kdr allele mutations. Ae aegypti samples were collected from several dengue endemic areas in West Sumatra. The susceptibility of adult mosquitos to insecticides was determined by bioassays using impregnation paper test with 0.18% malathion and 0.75% permethrin. In this study, we successfully confirmed that the A. populations have point mutations in the VGSC gene corresponding to S989P and V1016G amino acid substitutions. To genotype S989P and V1016G alleles, we designed kdr allele-specific primers based on the sequence of VGSC gene (NCBI Accession number No. EU399179.1. PCR analysis using the kdr allele-specific primers successfully identified the genotype of Ae. aegypti populations resistant to malathion and permethrin in West Sumatra.HASMIWATI HASMIWATISUPARGIYONO SUPARGIYONOMBI & UNS Soloarticleaedes aegyptiresistanceprimer designas-pcrkdr mutationBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 502-508 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	aedes aegypti resistance primer design as-pcr kdr mutation Biology (General) QH301-705.5
spellingShingle	aedes aegypti resistance primer design as-pcr kdr mutation Biology (General) QH301-705.5 HASMIWATI HASMIWATI SUPARGIYONO SUPARGIYONO Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
description	Hasmiwati, Supargiyono. 2018. Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia. Biodiversitas 19: 552-558. Aedes aegypti, the primary vector of Dengue fever, is widely distributed in Indonesia including in West Sumatra. Chemical insecticide is an effective way of shedding the chain of Dengue fever transmission. Long-term applications of insecticides have resulted in the development of resistance in Ae. aegypti populations. Knockdown resistance (kdr) allele as a result of point mutations has been found in Voltage-Gated Sodium Channel (VGSC) gene. This study aimed to design allele-specific primers to detect the kdr allele mutations. Ae aegypti samples were collected from several dengue endemic areas in West Sumatra. The susceptibility of adult mosquitos to insecticides was determined by bioassays using impregnation paper test with 0.18% malathion and 0.75% permethrin. In this study, we successfully confirmed that the A. populations have point mutations in the VGSC gene corresponding to S989P and V1016G amino acid substitutions. To genotype S989P and V1016G alleles, we designed kdr allele-specific primers based on the sequence of VGSC gene (NCBI Accession number No. EU399179.1. PCR analysis using the kdr allele-specific primers successfully identified the genotype of Ae. aegypti populations resistant to malathion and permethrin in West Sumatra.
format	article
author	HASMIWATI HASMIWATI SUPARGIYONO SUPARGIYONO
author_facet	HASMIWATI HASMIWATI SUPARGIYONO SUPARGIYONO
author_sort	HASMIWATI HASMIWATI
title	Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
title_short	Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
title_full	Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
title_fullStr	Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
title_full_unstemmed	Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia
title_sort	short communication: genotyping of kdr allele in insecticide resistant-aedes aegypti populations from west sumatra, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/145d62fd6c7842ada99e53c72f7d9bd0
work_keys_str_mv	AT hasmiwatihasmiwati shortcommunicationgenotypingofkdralleleininsecticideresistantaedesaegyptipopulationsfromwestsumatraindonesia AT supargiyonosupargiyono shortcommunicationgenotypingofkdralleleininsecticideresistantaedesaegyptipopulationsfromwestsumatraindonesia
_version_	1718426404545101824

Short Communication: Genotyping of kdr allele in insecticide resistant-Aedes aegypti populations from West Sumatra, Indonesia

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