Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.

TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Phuong Nguyen Duy, Dai Tran Lan, Hang Pham Thu, Huong Phung Thi Thu, Ha Nguyen Thanh, Ngoc Phuong Pham, Florence Auguy, Huong Bui Thi Thu, Tran Bao Manh, Sebastien Cunnac, Xuan Hoi Pham
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/145dce7282184ef4967d27e9f8990353
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:145dce7282184ef4967d27e9f8990353
record_format	dspace
spelling	oai:doaj.org-article:145dce7282184ef4967d27e9f89903532021-12-02T20:08:24ZImproved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.1932-620310.1371/journal.pone.0255470https://doaj.org/article/145dce7282184ef4967d27e9f89903532021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255470https://doaj.org/toc/1932-6203TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety.Phuong Nguyen DuyDai Tran LanHang Pham ThuHuong Phung Thi ThuHa Nguyen ThanhNgoc Phuong PhamFlorence AuguyHuong Bui Thi ThuTran Bao ManhSebastien CunnacXuan Hoi PhamPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0255470 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
description	TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. OsSWEET14 belongs to the SWEET gene family that encodes sugar transporters. Together with other Clade III members, it behaves as a susceptibility (S) gene whose induction by Asian Xoo Transcription-Activator-Like Effectors (TALEs) is absolutely necessary for disease. In this study, we sought to introduce BLB resistance in the TBR225 elite variety. First, two Vietnamese Xoo strains were shown to up-regulate OsSWEET14 upon TBR225 infection. To investigate if this induction is connected with disease susceptibility, nine TBR225 mutant lines with mutations in the AvrXa7, PthXo3 or TalF TALEs DNA target sequences of the OsSWEET14 promoter were obtained using the CRISPR/Cas9 editing system. Genotyping analysis of T0 and T1 individuals showed that mutations were stably inherited. None of the examined agronomic traits of three transgene-free T2 edited lines were significantly different from those of wild-type TBR225. Importantly, one of these T2 lines, harboring the largest homozygous 6-bp deletion, displayed decreased OsSWEET14 expression as well as a significantly reduced susceptibility to a Vietnamese Xoo strains and complete resistance to another one. Our findings indicate that CRISPR/Cas9 editing conferred an improved BLB resistance to a Vietnamese commercial elite rice variety.
format	article
author	Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham
author_facet	Phuong Nguyen Duy Dai Tran Lan Hang Pham Thu Huong Phung Thi Thu Ha Nguyen Thanh Ngoc Phuong Pham Florence Auguy Huong Bui Thi Thu Tran Bao Manh Sebastien Cunnac Xuan Hoi Pham
author_sort	Phuong Nguyen Duy
title	Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
title_short	Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
title_full	Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
title_fullStr	Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
title_full_unstemmed	Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.
title_sort	improved bacterial leaf blight disease resistance in the major elite vietnamese rice cultivar tbr225 via editing of the ossweet14 promoter.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/145dce7282184ef4967d27e9f8990353
work_keys_str_mv	AT phuongnguyenduy improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT daitranlan improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT hangphamthu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT huongphungthithu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT hanguyenthanh improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT ngocphuongpham improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT florenceauguy improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT huongbuithithu improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT tranbaomanh improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT sebastiencunnac improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter AT xuanhoipham improvedbacterialleafblightdiseaseresistanceinthemajorelitevietnamesericecultivartbr225viaeditingoftheossweet14promoter
_version_	1718375165517103104

Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.

Ejemplares similares