Microbiota dysbiosis and functional outcome in acute ischemic stroke patients

Abstract Currently, few studies are reported on the composition of microbiota in stroke patients and the association with stroke prognosis. This study investigated the differing microbiota composition in stroke patients and confirmed the association of microbiota composition with poor functional out...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yoonkyung Chang, Ho Geol Woo, Jee Hyang Jeong, Geon Ha Kim, Kee Duk Park, Tae-Jin Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14602572b7a041968808e9417d022367
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:14602572b7a041968808e9417d022367
record_format	dspace
spelling	oai:doaj.org-article:14602572b7a041968808e9417d0223672021-12-02T14:49:18ZMicrobiota dysbiosis and functional outcome in acute ischemic stroke patients10.1038/s41598-021-90463-52045-2322https://doaj.org/article/14602572b7a041968808e9417d0223672021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90463-5https://doaj.org/toc/2045-2322Abstract Currently, few studies are reported on the composition of microbiota in stroke patients and the association with stroke prognosis. This study investigated the differing microbiota composition in stroke patients and confirmed the association of microbiota composition with poor functional outcome. Between January of 2018 and December of 2019, 198 patients with acute cerebral infarction were included in this study. For the case–control study, age and sex-matched normal healthy subjects (n = 200) were included when receiving their health screening examinations. We isolated bacterial extracellular membrane vesicles and extracted DNA from blood samples. Taxonomic assignments were performed by using the sequence reads of 16S rRNA genes following blood microbiota analysis. Statistical analysis was conducted appropriately by using Statistical Analysis System software. The mean age of the stroke patients were 63.7 ± 12.5 years, and the male sex was 58.5%. Of the total enrolled patients, poor functional outcome (modified Rankin Score ≥ 3) was noted in 19.7%. The principal component analysis of microbiota composition revealed significant differences between healthy control subjects and stroke patients. At the genus level, Aerococcaceae(f), ZB2(c), TM7-1(c), and Flavobacterium were significantly increased in stroke patients compared to the healthy controls, whereas Mucispirillum, rc4-4, Akkermansia, Clostridiales(o), Lactobacillus, and Stenotrophomonas were decreased considerably. For the functional outcome after ischemic stroke, Anaerococcus, Blautia, Dialister, Aerococcaceae(f), Propionibacterium, Microbacteriaceae(f), and Rothia were enriched in the group with good outcomes, whereas Ruminococcaceae(f) and Prevotella were enriched in the group with poor outcome. There was apparent dysbiosis of blood microbiota in patients with acute ischemic stroke compared to healthy people. Ruminococcaceae(f) and Prevotella were elevated in stroke patients with poor functional outcome.Yoonkyung ChangHo Geol WooJee Hyang JeongGeon Ha KimKee Duk ParkTae-Jin SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoonkyung Chang Ho Geol Woo Jee Hyang Jeong Geon Ha Kim Kee Duk Park Tae-Jin Song Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
description	Abstract Currently, few studies are reported on the composition of microbiota in stroke patients and the association with stroke prognosis. This study investigated the differing microbiota composition in stroke patients and confirmed the association of microbiota composition with poor functional outcome. Between January of 2018 and December of 2019, 198 patients with acute cerebral infarction were included in this study. For the case–control study, age and sex-matched normal healthy subjects (n = 200) were included when receiving their health screening examinations. We isolated bacterial extracellular membrane vesicles and extracted DNA from blood samples. Taxonomic assignments were performed by using the sequence reads of 16S rRNA genes following blood microbiota analysis. Statistical analysis was conducted appropriately by using Statistical Analysis System software. The mean age of the stroke patients were 63.7 ± 12.5 years, and the male sex was 58.5%. Of the total enrolled patients, poor functional outcome (modified Rankin Score ≥ 3) was noted in 19.7%. The principal component analysis of microbiota composition revealed significant differences between healthy control subjects and stroke patients. At the genus level, Aerococcaceae(f), ZB2(c), TM7-1(c), and Flavobacterium were significantly increased in stroke patients compared to the healthy controls, whereas Mucispirillum, rc4-4, Akkermansia, Clostridiales(o), Lactobacillus, and Stenotrophomonas were decreased considerably. For the functional outcome after ischemic stroke, Anaerococcus, Blautia, Dialister, Aerococcaceae(f), Propionibacterium, Microbacteriaceae(f), and Rothia were enriched in the group with good outcomes, whereas Ruminococcaceae(f) and Prevotella were enriched in the group with poor outcome. There was apparent dysbiosis of blood microbiota in patients with acute ischemic stroke compared to healthy people. Ruminococcaceae(f) and Prevotella were elevated in stroke patients with poor functional outcome.
format	article
author	Yoonkyung Chang Ho Geol Woo Jee Hyang Jeong Geon Ha Kim Kee Duk Park Tae-Jin Song
author_facet	Yoonkyung Chang Ho Geol Woo Jee Hyang Jeong Geon Ha Kim Kee Duk Park Tae-Jin Song
author_sort	Yoonkyung Chang
title	Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
title_short	Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
title_full	Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
title_fullStr	Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
title_full_unstemmed	Microbiota dysbiosis and functional outcome in acute ischemic stroke patients
title_sort	microbiota dysbiosis and functional outcome in acute ischemic stroke patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/14602572b7a041968808e9417d022367
work_keys_str_mv	AT yoonkyungchang microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients AT hogeolwoo microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients AT jeehyangjeong microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients AT geonhakim microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients AT keedukpark microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients AT taejinsong microbiotadysbiosisandfunctionaloutcomeinacuteischemicstrokepatients
_version_	1718389493093892096

Microbiota dysbiosis and functional outcome in acute ischemic stroke patients

Ejemplares similares