In Vitro Anti-Epstein Barr Virus Activity of Olea europaea L. Leaf Extracts

Olea europaea L. var. sativa (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxi...

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Autores principales:	Ichrak Ben-Amor, Bochra Gargouri, Hamadi Attia, Khaoula Tlili, Imen Kallel, Maria Musarra-Pizzo, Maria Teresa Sciortino, Rosamaria Pennisi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Olea europaea</i> leaves antivirals antioxidant plants bioactive compounds Botany QK1-989
Acceso en línea:	https://doaj.org/article/14623be7920b4ce5bf74dcbbcf4e0002
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spelling	oai:doaj.org-article:14623be7920b4ce5bf74dcbbcf4e00022021-11-25T18:46:39ZIn Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts10.3390/plants101124452223-7747https://doaj.org/article/14623be7920b4ce5bf74dcbbcf4e00022021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2445https://doaj.org/toc/2223-7747<i>Olea europaea</i> L. var. <i>sativa</i> (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC<sub>50</sub> values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (<i>p</i> < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** <i>p</i> < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.Ichrak Ben-AmorBochra GargouriHamadi AttiaKhaoula TliliImen KallelMaria Musarra-PizzoMaria Teresa SciortinoRosamaria PennisiMDPI AGarticle<i>Olea europaea</i> leavesantiviralsantioxidantplants bioactive compoundsBotanyQK1-989ENPlants, Vol 10, Iss 2445, p 2445 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Olea europaea</i> leaves antivirals antioxidant plants bioactive compounds Botany QK1-989
spellingShingle	<i>Olea europaea</i> leaves antivirals antioxidant plants bioactive compounds Botany QK1-989 Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
description	<i>Olea europaea</i> L. var. <i>sativa</i> (OESA) preparations are widely used in traditional medicine in the Mediterranean region to prevent and treat different diseases. In this research, olive extracts derived from the leaves of the OESA tree have been screened for antioxidant activity by two methods: the DPPH free radical scavenging assay (DPPH) and the Ferric reducing antioxidant power (FRAP) assay. The DPPH assay showed that OESA possesses a stronger antioxidant activity (84%) at 1 mg/mL while the FRAP method showed a strong metal ion chelating activity (90%) at 1 mg/mL. The low IC<sub>50</sub> values, obtained by two different methods, implies that OESA has a noticeable effect on scavenging free radicals comparable to standards. During EBV infection, the free radicals increased triggering lipid oxidation. Therefore, the monitoring of the secondary lipid peroxidation products was done by measuring malonaldehyde (MDA) and conjugated dienes (DC). The simultaneous treatment of Raji cells with OESA and TPA, as an inductorof the lytic cycle, generated a significant decrease in MDA levels and DC (<i>p</i> < 0.05). Besides, Raji cells simultaneously exposed to TPA and OESA exhibited a percentage of EBV-positive fluorescence cells lower than TPA treated cells (**** <i>p</i> < 0.0001). This suggests that OESA treatment has a protective effect against EBV lytic cycle induction.
format	article
author	Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi
author_facet	Ichrak Ben-Amor Bochra Gargouri Hamadi Attia Khaoula Tlili Imen Kallel Maria Musarra-Pizzo Maria Teresa Sciortino Rosamaria Pennisi
author_sort	Ichrak Ben-Amor
title	In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
title_short	In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
title_full	In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
title_fullStr	In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
title_full_unstemmed	In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts
title_sort	in vitro anti-epstein barr virus activity of <i>olea europaea</i> l. leaf extracts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/14623be7920b4ce5bf74dcbbcf4e0002
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In Vitro Anti-Epstein Barr Virus Activity of <i>Olea europaea</i> L. Leaf Extracts

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