Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK

Phenolic compounds are chemical precursor building blocks of soil organic matter. Their occurrence can be inhibitory to certain enzymes present in soil, thereby influencing the rate of decomposition of soil organic matter. Microbe-derived phenoloxidases (laccases) are extracellular enzymes capable o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Benneth O. I. Esiana, Christopher J. Coates, W. Paul Adderley, Anne E. Berns, Roland Bol
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1464a292f5034a729d2f25447b095a1e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1464a292f5034a729d2f25447b095a1e
record_format	dspace
spelling	oai:doaj.org-article:1464a292f5034a729d2f25447b095a1e2021-11-04T06:49:45ZPhenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK1932-6203https://doaj.org/article/1464a292f5034a729d2f25447b095a1e2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550383/?tool=EBIhttps://doaj.org/toc/1932-6203Phenolic compounds are chemical precursor building blocks of soil organic matter. Their occurrence can be inhibitory to certain enzymes present in soil, thereby influencing the rate of decomposition of soil organic matter. Microbe-derived phenoloxidases (laccases) are extracellular enzymes capable of degrading recalcitrant polyphenolic compounds. In this study, our aim was to investigate the relationships between phenoloxidase enzyme activity, organic carbon content and microbial abundance in the context of long-term anthropogenically amended soils. To achieve this, we used a series of complementary biochemical analytical methods including gas chromatography, enzyme assays and solid-state Carbon-13 Cross Polarisation Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy (13C CPMAS NMR). Using several anthrosols found in St Andrews (Scotland, UK) that had been subjected to intense anthropogenic modification since the medieval period (11th century AD) to present-day, we were able to scope the impact of past waste disposal on soils. The long-term anthropogenic impact led to organic matter-rich soils. Overall, phenoloxidase activity increased by up to 2-fold with soil depth (up to 100 cm) and was inversely correlated with microbial biomass. Solid-state 13C NMR characterisation of carbon species revealed that the observed decline in soil organic matter with depth corresponded to decreases in the labile organic carbon fractions as evidenced by changes in the O/N-alkyl C region of the spectra. The increase in phenoloxidase activity with depth would appear to be a compensatory mechanism for the reduced quantities of organic carbon and lower overall nutrient environment in subsoils. By enzymatically targeting phenolic compounds, microbes can better utilise recalcitrant carbon when other labile soil carbon sources become limited, thereby maintaining metabolic processes.Benneth O. I. EsianaChristopher J. CoatesW. Paul AdderleyAnne E. BernsRoland BolPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benneth O. I. Esiana Christopher J. Coates W. Paul Adderley Anne E. Berns Roland Bol Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
description	Phenolic compounds are chemical precursor building blocks of soil organic matter. Their occurrence can be inhibitory to certain enzymes present in soil, thereby influencing the rate of decomposition of soil organic matter. Microbe-derived phenoloxidases (laccases) are extracellular enzymes capable of degrading recalcitrant polyphenolic compounds. In this study, our aim was to investigate the relationships between phenoloxidase enzyme activity, organic carbon content and microbial abundance in the context of long-term anthropogenically amended soils. To achieve this, we used a series of complementary biochemical analytical methods including gas chromatography, enzyme assays and solid-state Carbon-13 Cross Polarisation Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy (13C CPMAS NMR). Using several anthrosols found in St Andrews (Scotland, UK) that had been subjected to intense anthropogenic modification since the medieval period (11th century AD) to present-day, we were able to scope the impact of past waste disposal on soils. The long-term anthropogenic impact led to organic matter-rich soils. Overall, phenoloxidase activity increased by up to 2-fold with soil depth (up to 100 cm) and was inversely correlated with microbial biomass. Solid-state 13C NMR characterisation of carbon species revealed that the observed decline in soil organic matter with depth corresponded to decreases in the labile organic carbon fractions as evidenced by changes in the O/N-alkyl C region of the spectra. The increase in phenoloxidase activity with depth would appear to be a compensatory mechanism for the reduced quantities of organic carbon and lower overall nutrient environment in subsoils. By enzymatically targeting phenolic compounds, microbes can better utilise recalcitrant carbon when other labile soil carbon sources become limited, thereby maintaining metabolic processes.
format	article
author	Benneth O. I. Esiana Christopher J. Coates W. Paul Adderley Anne E. Berns Roland Bol
author_facet	Benneth O. I. Esiana Christopher J. Coates W. Paul Adderley Anne E. Berns Roland Bol
author_sort	Benneth O. I. Esiana
title	Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
title_short	Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
title_full	Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
title_fullStr	Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
title_full_unstemmed	Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK
title_sort	phenoloxidase activity and organic carbon dynamics in historic anthrosols in scotland, uk
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1464a292f5034a729d2f25447b095a1e
work_keys_str_mv	AT bennethoiesiana phenoloxidaseactivityandorganiccarbondynamicsinhistoricanthrosolsinscotlanduk AT christopherjcoates phenoloxidaseactivityandorganiccarbondynamicsinhistoricanthrosolsinscotlanduk AT wpauladderley phenoloxidaseactivityandorganiccarbondynamicsinhistoricanthrosolsinscotlanduk AT anneeberns phenoloxidaseactivityandorganiccarbondynamicsinhistoricanthrosolsinscotlanduk AT rolandbol phenoloxidaseactivityandorganiccarbondynamicsinhistoricanthrosolsinscotlanduk
_version_	1718445004877201408

Phenoloxidase activity and organic carbon dynamics in historic Anthrosols in Scotland, UK

Ejemplares similares