Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil

Background: Piracanjuba (Brycon orbignyanus) is a fish species highly affected by anthropogenic actions such as overfishing, water pollution, and hydroelectric developments. This species is currently considered in danger of extinction. Objective: To analyze the genetic diversity of a natural populat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nelson M. Lopera-Barrero, Silvio C. A. Santos, Pedro L. Castro, Felipe P. Souza, Angela R. Poveda-Parra, Victor C. F. Pandolfi, Andrei L. Yamachita, Angela M. Urrea-Rojas, Diego A. Rojas-Meza, Ricardo P. Ribeiro
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	aquaculture brycon orbignyanus genetic conservation microsatellites rapd Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/146e5afa5f254b609cb8ec15981fc155
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:146e5afa5f254b609cb8ec15981fc155
record_format	dspace
spelling	oai:doaj.org-article:146e5afa5f254b609cb8ec15981fc1552021-12-01T18:59:21ZGenetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil2256-295810.17533/udea.rccp.v32n2a07https://doaj.org/article/146e5afa5f254b609cb8ec15981fc1552019-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/335846https://doaj.org/toc/2256-2958Background: Piracanjuba (Brycon orbignyanus) is a fish species highly affected by anthropogenic actions such as overfishing, water pollution, and hydroelectric developments. This species is currently considered in danger of extinction. Objective: To analyze the genetic diversity of a natural population (NP) and two captive broodstocks (SA and SB) of B. orbignyanus. Methods: Samples of caudal fins (NP: 24, SA: 30, and SB: 30) were collected. DNA was extracted and amplified for six RAPD primers and four microsatellite loci. Results: Sixty polymorphic fragments and 17 microsatellite alleles were detected. High intrapopulation heterozygosity (NP: 0.692, SA: 0.724, and SB: 0.686) was observed. Thirty-eight fragments and six alleles were shared among NP, SA, and SB. The FIS and Shannon’s Index of diversity revealed a lack of inbreeding within groups. AMOVA analyses and FST indicated very high (NP vs SA and SB) and small (SA vs SB) genetic differentiation, confirmed by genetic distance and identity, number of migrants and a dendrogram, which revealed the formation of two genetic groups. Conclusions: The two marker types showed similar variability. The groups have adequate genetic variability, with high differentiation between NP and SA–SB, and similarity between broodstocks.Nelson M. Lopera-BarreroSilvio C. A. SantosPedro L. CastroFelipe P. SouzaAngela R. Poveda-ParraVictor C. F. PandolfiAndrei L. YamachitaAngela M. Urrea-RojasDiego A. Rojas-MezaRicardo P. RibeiroUniversidad de Antioquiaarticleaquaculturebrycon orbignyanusgenetic conservationmicrosatellitesrapdAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 2, Pp 139-149 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquaculture brycon orbignyanus genetic conservation microsatellites rapd Animal culture SF1-1100
spellingShingle	aquaculture brycon orbignyanus genetic conservation microsatellites rapd Animal culture SF1-1100 Nelson M. Lopera-Barrero Silvio C. A. Santos Pedro L. Castro Felipe P. Souza Angela R. Poveda-Parra Victor C. F. Pandolfi Andrei L. Yamachita Angela M. Urrea-Rojas Diego A. Rojas-Meza Ricardo P. Ribeiro Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
description	Background: Piracanjuba (Brycon orbignyanus) is a fish species highly affected by anthropogenic actions such as overfishing, water pollution, and hydroelectric developments. This species is currently considered in danger of extinction. Objective: To analyze the genetic diversity of a natural population (NP) and two captive broodstocks (SA and SB) of B. orbignyanus. Methods: Samples of caudal fins (NP: 24, SA: 30, and SB: 30) were collected. DNA was extracted and amplified for six RAPD primers and four microsatellite loci. Results: Sixty polymorphic fragments and 17 microsatellite alleles were detected. High intrapopulation heterozygosity (NP: 0.692, SA: 0.724, and SB: 0.686) was observed. Thirty-eight fragments and six alleles were shared among NP, SA, and SB. The FIS and Shannon’s Index of diversity revealed a lack of inbreeding within groups. AMOVA analyses and FST indicated very high (NP vs SA and SB) and small (SA vs SB) genetic differentiation, confirmed by genetic distance and identity, number of migrants and a dendrogram, which revealed the formation of two genetic groups. Conclusions: The two marker types showed similar variability. The groups have adequate genetic variability, with high differentiation between NP and SA–SB, and similarity between broodstocks.
format	article
author	Nelson M. Lopera-Barrero Silvio C. A. Santos Pedro L. Castro Felipe P. Souza Angela R. Poveda-Parra Victor C. F. Pandolfi Andrei L. Yamachita Angela M. Urrea-Rojas Diego A. Rojas-Meza Ricardo P. Ribeiro
author_facet	Nelson M. Lopera-Barrero Silvio C. A. Santos Pedro L. Castro Felipe P. Souza Angela R. Poveda-Parra Victor C. F. Pandolfi Andrei L. Yamachita Angela M. Urrea-Rojas Diego A. Rojas-Meza Ricardo P. Ribeiro
author_sort	Nelson M. Lopera-Barrero
title	Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
title_short	Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
title_full	Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
title_fullStr	Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
title_full_unstemmed	Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil
title_sort	genetic diversity of piracanjuba populations in fish stocking programs in the tietê river, brazil
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/146e5afa5f254b609cb8ec15981fc155
work_keys_str_mv	AT nelsonmloperabarrero geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT silviocasantos geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT pedrolcastro geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT felipepsouza geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT angelarpovedaparra geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT victorcfpandolfi geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT andreilyamachita geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT angelamurrearojas geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT diegoarojasmeza geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil AT ricardopribeiro geneticdiversityofpiracanjubapopulationsinfishstockingprogramsinthetieteriverbrazil
_version_	1718404617221439488

Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil

Ejemplares similares