Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells

Ana Carolina Martínez-Torres*, Helen Yarimet Lorenzo-Anota*, Martín Gerardo García-Juárez*, Diana G Zarate-Triviño, Cristina Rodríguez-Padilla Universidad Autónoma De Nuevo León, Facultad De Ciencias Biol&oac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martínez-Torres AC, Lorenzo-Anota HY, García-Juárez MG, Zarate-Triviño DG, Rodríguez-Padilla C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	AuNPs leukemia nuclear alterations apoptosis necroptosis autophagy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1478b50154924f7093e6863a7791dce4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1478b50154924f7093e6863a7791dce4
record_format	dspace
spelling	oai:doaj.org-article:1478b50154924f7093e6863a7791dce42021-12-02T11:33:05ZChitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells1178-2013https://doaj.org/article/1478b50154924f7093e6863a7791dce42019-09-01T00:00:00Zhttps://www.dovepress.com/chitosan-gold-nanoparticles-induce-different-ros-dependent-cell-death--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ana Carolina Martínez-Torres, Helen Yarimet Lorenzo-Anota, Martín Gerardo García-Juárez, Diana G Zarate-Triviño, Cristina Rodríguez-Padilla Universidad Autónoma De Nuevo León, Facultad De Ciencias Biológicas, Laboratorio De Inmunología Y Virología, Monterrey, Nuevo Leon, MexicoThese authors contributed equally to this workCorrespondence: Ana Carolina Martínez-TorresUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Inmunología y Virología, Monterrey 66455, MéxicoTel +52 8 121 4115Fax +52 818 352 4212Email ana.martinezto@uanl.edu.mxBackground: Nanotechnology proposes the use of gold nanoparticles (AuNPs) for drug delivery, diagnosis, and treatment of cancer. Leukemia is a type of hematopoietic cancer that results from the malignant transformation of white blood cells. Chitosan-coated AuNPs (CH-AuNPs) are cell death inductors in HeLa and MCF-7 cancer cells without affecting peripheral blood mononuclear cells (PBMC). Considering the selectivity and versatile cytotoxicity of CH-AuNPs, we evaluated whether their selectivity is due to the cell lineage or the characteristics of the cancer cells, by assessing its cytotoxicity in leukemic cells. Moreover, we further examined the cell death mechanism and assessed the implication of nuclear damage, autophagosome formation, and the cell death mechanism induced in leukemic cells.Materials and methods: We synthesized CH-AuNPs by chemical methods and analyzed their cell death capacity in a T-acute lymphocytic leukemia cell line (CEM), in a chronic myeloid leukemia cell line (K562), and in healthy cells from the same lineage (PBMC and bone marrow, BM, cells). Then, we assessed ROS generation and mitochondrial and nuclear damage. Finally, we evaluated whether cell death occurred by autophagy, apoptosis, or necroptosis, and the role of ROS in this mechanism.Results: We found that CH-AuNPs did not affect PBMC and BM cells, whereas they are cytotoxic in a dose-dependent manner in leukemic cells. ROS production leads to mitochondrial and nuclear damage, and cell death. We found that CH-AuNPs induce apoptosis in CEM and necroptosis in K562, both undergoing autophagy as a pro-survival mechanism.Conclusion: CH-AuNPs are selective cell death inductors in hematologic cancer cells, without affecting their healthy counterparts. Cell death induced by CH-AuNPs is independent of the cancer cell type; however, its mechanism is different depending on the type of leukemic cells.Keywords: AuNPs, leukemia, nuclear alterations, apoptosis, necroptosis, autophagyMartínez-Torres ACLorenzo-Anota HYGarcía-Juárez MGZarate-Triviño DGRodríguez-Padilla CDove Medical PressarticleAuNPsleukemianuclear alterationsapoptosisnecroptosisautophagyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 7173-7190 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	AuNPs leukemia nuclear alterations apoptosis necroptosis autophagy Medicine (General) R5-920
spellingShingle	AuNPs leukemia nuclear alterations apoptosis necroptosis autophagy Medicine (General) R5-920 Martínez-Torres AC Lorenzo-Anota HY García-Juárez MG Zarate-Triviño DG Rodríguez-Padilla C Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
description	Ana Carolina Martínez-Torres, Helen Yarimet Lorenzo-Anota, Martín Gerardo García-Juárez, Diana G Zarate-Triviño, Cristina Rodríguez-Padilla Universidad Autónoma De Nuevo León, Facultad De Ciencias Biológicas, Laboratorio De Inmunología Y Virología, Monterrey, Nuevo Leon, MexicoThese authors contributed equally to this workCorrespondence: Ana Carolina Martínez-TorresUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Inmunología y Virología, Monterrey 66455, MéxicoTel +52 8 121 4115Fax +52 818 352 4212Email ana.martinezto@uanl.edu.mxBackground: Nanotechnology proposes the use of gold nanoparticles (AuNPs) for drug delivery, diagnosis, and treatment of cancer. Leukemia is a type of hematopoietic cancer that results from the malignant transformation of white blood cells. Chitosan-coated AuNPs (CH-AuNPs) are cell death inductors in HeLa and MCF-7 cancer cells without affecting peripheral blood mononuclear cells (PBMC). Considering the selectivity and versatile cytotoxicity of CH-AuNPs, we evaluated whether their selectivity is due to the cell lineage or the characteristics of the cancer cells, by assessing its cytotoxicity in leukemic cells. Moreover, we further examined the cell death mechanism and assessed the implication of nuclear damage, autophagosome formation, and the cell death mechanism induced in leukemic cells.Materials and methods: We synthesized CH-AuNPs by chemical methods and analyzed their cell death capacity in a T-acute lymphocytic leukemia cell line (CEM), in a chronic myeloid leukemia cell line (K562), and in healthy cells from the same lineage (PBMC and bone marrow, BM, cells). Then, we assessed ROS generation and mitochondrial and nuclear damage. Finally, we evaluated whether cell death occurred by autophagy, apoptosis, or necroptosis, and the role of ROS in this mechanism.Results: We found that CH-AuNPs did not affect PBMC and BM cells, whereas they are cytotoxic in a dose-dependent manner in leukemic cells. ROS production leads to mitochondrial and nuclear damage, and cell death. We found that CH-AuNPs induce apoptosis in CEM and necroptosis in K562, both undergoing autophagy as a pro-survival mechanism.Conclusion: CH-AuNPs are selective cell death inductors in hematologic cancer cells, without affecting their healthy counterparts. Cell death induced by CH-AuNPs is independent of the cancer cell type; however, its mechanism is different depending on the type of leukemic cells.Keywords: AuNPs, leukemia, nuclear alterations, apoptosis, necroptosis, autophagy
format	article
author	Martínez-Torres AC Lorenzo-Anota HY García-Juárez MG Zarate-Triviño DG Rodríguez-Padilla C
author_facet	Martínez-Torres AC Lorenzo-Anota HY García-Juárez MG Zarate-Triviño DG Rodríguez-Padilla C
author_sort	Martínez-Torres AC
title	Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
title_short	Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
title_full	Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
title_fullStr	Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
title_full_unstemmed	Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells
title_sort	chitosan gold nanoparticles induce different ros-dependent cell death modalities in leukemic cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/1478b50154924f7093e6863a7791dce4
work_keys_str_mv	AT martineztorresac chitosangoldnanoparticlesinducedifferentrosdependentcelldeathmodalitiesinleukemiccells AT lorenzoanotahy chitosangoldnanoparticlesinducedifferentrosdependentcelldeathmodalitiesinleukemiccells AT garciajuarezmg chitosangoldnanoparticlesinducedifferentrosdependentcelldeathmodalitiesinleukemiccells AT zaratetrivinodg chitosangoldnanoparticlesinducedifferentrosdependentcelldeathmodalitiesinleukemiccells AT rodriguezpadillac chitosangoldnanoparticlesinducedifferentrosdependentcelldeathmodalitiesinleukemiccells
_version_	1718395805309599744

Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells

Ejemplares similares