Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage

Establishing an in vitro–based cell culture system that can realistically simulate in vivo cell dynamics is desirable. It is thus necessary to develop a method for producing a large amount of cell aggregates (i.e., spheroids) that are uniform in size and quality. Various methods have been proposed f...

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Autores principales:	Hidetaka Togo, Kento Yoshikawa-Terada, Yudai Hirose, Hideo Nakagawa, Hiroki Takeuchi, Masanobu Kusunoki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	spheroid organoid van der Waals force fluoropolymers adhesion microstamp Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/147aa2c500064286bde5c721c4749d50
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spelling	oai:doaj.org-article:147aa2c500064286bde5c721c4749d502021-11-11T15:25:54ZDevelopment of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage10.3390/app1121104952076-3417https://doaj.org/article/147aa2c500064286bde5c721c4749d502021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10495https://doaj.org/toc/2076-3417Establishing an in vitro–based cell culture system that can realistically simulate in vivo cell dynamics is desirable. It is thus necessary to develop a method for producing a large amount of cell aggregates (i.e., spheroids) that are uniform in size and quality. Various methods have been proposed for the preparation of spheroids; however, none of them satisfy all requirements, such as cost, size uniformity, and throughput. Herein, we successfully developed a new cell culture method by combining fluoropolymers and dot patterned extracellular matrix substrates to achieve size-controlled spheroids. First, the spheroids were spontaneously formed by culturing them two-dimensionally, after which the cells were detached with a weak liquid flow and cultured in suspension without enzyme treatment. Stable quality spheroids were easily produced, and it is expected that the introduction and running costs of the technique will be low; therefore, this method shows potential for application in the field of regenerative medicine.Hidetaka TogoKento Yoshikawa-TeradaYudai HiroseHideo NakagawaHiroki TakeuchiMasanobu KusunokiMDPI AGarticlespheroidorganoidvan der Waals forcefluoropolymersadhesionmicrostampTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10495, p 10495 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spheroid organoid van der Waals force fluoropolymers adhesion microstamp Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	spheroid organoid van der Waals force fluoropolymers adhesion microstamp Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Hidetaka Togo Kento Yoshikawa-Terada Yudai Hirose Hideo Nakagawa Hiroki Takeuchi Masanobu Kusunoki Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
description	Establishing an in vitro–based cell culture system that can realistically simulate in vivo cell dynamics is desirable. It is thus necessary to develop a method for producing a large amount of cell aggregates (i.e., spheroids) that are uniform in size and quality. Various methods have been proposed for the preparation of spheroids; however, none of them satisfy all requirements, such as cost, size uniformity, and throughput. Herein, we successfully developed a new cell culture method by combining fluoropolymers and dot patterned extracellular matrix substrates to achieve size-controlled spheroids. First, the spheroids were spontaneously formed by culturing them two-dimensionally, after which the cells were detached with a weak liquid flow and cultured in suspension without enzyme treatment. Stable quality spheroids were easily produced, and it is expected that the introduction and running costs of the technique will be low; therefore, this method shows potential for application in the field of regenerative medicine.
format	article
author	Hidetaka Togo Kento Yoshikawa-Terada Yudai Hirose Hideo Nakagawa Hiroki Takeuchi Masanobu Kusunoki
author_facet	Hidetaka Togo Kento Yoshikawa-Terada Yudai Hirose Hideo Nakagawa Hiroki Takeuchi Masanobu Kusunoki
author_sort	Hidetaka Togo
title	Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
title_short	Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
title_full	Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
title_fullStr	Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
title_full_unstemmed	Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage
title_sort	development of a simple spheroid production method using fluoropolymers with reduced chemical and physical damage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/147aa2c500064286bde5c721c4749d50
work_keys_str_mv	AT hidetakatogo developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage AT kentoyoshikawaterada developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage AT yudaihirose developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage AT hideonakagawa developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage AT hirokitakeuchi developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage AT masanobukusunoki developmentofasimplespheroidproductionmethodusingfluoropolymerswithreducedchemicalandphysicaldamage
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Development of a Simple Spheroid Production Method Using Fluoropolymers with Reduced Chemical and Physical Damage

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