Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage

Abstract Ektacytometry has been the primary method for evaluating deformability of red blood cells (RBCs) in both research and clinical settings. This study was designed to test the hypothesis that the flow of RBCs through a network of microfluidic capillaries could provide a more sensitive assessme...

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Autores principales:	Nathaniel Z. Piety, Julianne Stutz, Nida Yilmaz, Hui Xia, Tatsuro Yoshida, Sergey S. Shevkoplyas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/147ac28da7b24f78a514006a1cd0d519
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spelling	oai:doaj.org-article:147ac28da7b24f78a514006a1cd0d5192021-12-02T14:02:33ZMicrofluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage10.1038/s41598-020-79710-32045-2322https://doaj.org/article/147ac28da7b24f78a514006a1cd0d5192021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79710-3https://doaj.org/toc/2045-2322Abstract Ektacytometry has been the primary method for evaluating deformability of red blood cells (RBCs) in both research and clinical settings. This study was designed to test the hypothesis that the flow of RBCs through a network of microfluidic capillaries could provide a more sensitive assessment of the progressive impairment of RBC deformability during hypothermic storage than ektacytometry. RBC units (n = 9) were split in half, with one half stored under standard (normoxic) conditions and the other half stored hypoxically, for up to 6 weeks. RBC deformability was measured weekly using two microfluidic devices, an artificial microvascular network (AMVN) and a multiplexed microcapillary network (MMCN), and two commercially available ektacytometers (RheoScan-D and LORRCA). By week 6, the elongation indexes measured with RheoScan-D and LORRCA decreased by 5.8–7.1% (5.4–6.9% for hypoxic storage). Over the same storage duration, the AMVN perfusion rate declined by 27.5% (24.5% for hypoxic) and the MMCN perfusion rate declined by 49.0% (42.4% for hypoxic). Unlike ektacytometry, both AMVN and MMCN measurements showed statistically significant differences between the two conditions after 1 week of storage. RBC morphology deteriorated continuously with the fraction of irreversibly-damaged (spherical) cells increasing significantly faster for normoxic than for hypoxic storage. Consequently, the number of MMCN capillary plugging events and the time MMCN capillaries spent plugged was consistently lower for hypoxic than for normoxic storage. These data suggest that capillary networks are significantly more sensitive to both the overall storage-induced decline of RBC deformability, and to the differences between the two storage conditions, than ektacytometry.Nathaniel Z. PietyJulianne StutzNida YilmazHui XiaTatsuro YoshidaSergey S. ShevkoplyasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathaniel Z. Piety Julianne Stutz Nida Yilmaz Hui Xia Tatsuro Yoshida Sergey S. Shevkoplyas Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
description	Abstract Ektacytometry has been the primary method for evaluating deformability of red blood cells (RBCs) in both research and clinical settings. This study was designed to test the hypothesis that the flow of RBCs through a network of microfluidic capillaries could provide a more sensitive assessment of the progressive impairment of RBC deformability during hypothermic storage than ektacytometry. RBC units (n = 9) were split in half, with one half stored under standard (normoxic) conditions and the other half stored hypoxically, for up to 6 weeks. RBC deformability was measured weekly using two microfluidic devices, an artificial microvascular network (AMVN) and a multiplexed microcapillary network (MMCN), and two commercially available ektacytometers (RheoScan-D and LORRCA). By week 6, the elongation indexes measured with RheoScan-D and LORRCA decreased by 5.8–7.1% (5.4–6.9% for hypoxic storage). Over the same storage duration, the AMVN perfusion rate declined by 27.5% (24.5% for hypoxic) and the MMCN perfusion rate declined by 49.0% (42.4% for hypoxic). Unlike ektacytometry, both AMVN and MMCN measurements showed statistically significant differences between the two conditions after 1 week of storage. RBC morphology deteriorated continuously with the fraction of irreversibly-damaged (spherical) cells increasing significantly faster for normoxic than for hypoxic storage. Consequently, the number of MMCN capillary plugging events and the time MMCN capillaries spent plugged was consistently lower for hypoxic than for normoxic storage. These data suggest that capillary networks are significantly more sensitive to both the overall storage-induced decline of RBC deformability, and to the differences between the two storage conditions, than ektacytometry.
format	article
author	Nathaniel Z. Piety Julianne Stutz Nida Yilmaz Hui Xia Tatsuro Yoshida Sergey S. Shevkoplyas
author_facet	Nathaniel Z. Piety Julianne Stutz Nida Yilmaz Hui Xia Tatsuro Yoshida Sergey S. Shevkoplyas
author_sort	Nathaniel Z. Piety
title	Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
title_short	Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
title_full	Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
title_fullStr	Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
title_full_unstemmed	Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
title_sort	microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/147ac28da7b24f78a514006a1cd0d519
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Microfluidic capillary networks are more sensitive than ektacytometry to the decline of red blood cell deformability induced by storage

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