Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members

Pancreatic cancers are among of the most lethal types of neoplasms, and are mostly detected at an advanced stage. Conventional treatment methods such as chemotherapy or radiotherapy often do not bring the desired therapeutic effects. For this reason, natural compounds are increasingly being used as...

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Autores principales:	Katarzyna Ratajczak, Natalia Glatzel-Plucińska, Katarzyna Ratajczak-Wielgomas, Katarzyna Nowińska, Sylwia Borska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	resveratrol Bax Bcl-2 Caspase-3 apoptosis multidrug resistance Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/148c960df9bc4ddeae858b2d1608b61e
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spelling	oai:doaj.org-article:148c960df9bc4ddeae858b2d1608b61e2021-11-11T18:32:36ZEffect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members10.3390/molecules262165601420-3049https://doaj.org/article/148c960df9bc4ddeae858b2d1608b61e2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6560https://doaj.org/toc/1420-3049Pancreatic cancers are among of the most lethal types of neoplasms, and are mostly detected at an advanced stage. Conventional treatment methods such as chemotherapy or radiotherapy often do not bring the desired therapeutic effects. For this reason, natural compounds are increasingly being used as adjuvants in cancer therapy. Polyphenolic compounds, including resveratrol, are of particular interest. The aim of this study is to analyze the antiproliferative and pro-apoptotic mechanisms of resveratrol on human pancreatic cells. The study was carried out on three human pancreatic cancer cell lines: EPP85-181P, EPP85-181RNOV (mitoxantrone-resistant cells) and AsPC-1, as well as the normal pancreatic cell line H6c7. The cytotoxicity of resveratrol in the tested cell lines was assessed by the colorimetric method (MTT) and the flow cytometry method. Three selected concentrations of the compound (25, 50 and 100 µM) were tested in the experiments during a 48-h incubation. TUNEL and Comet assays, flow cytometry, immunocytochemistry, confocal microscopy, real-time PCR and Western Blot analyses were used to evaluate the pleiotropic effect of resveratrol. The results indicate that resveratrol is likely to be anticarcinogenic by inhibiting human pancreatic cancer cell proliferation. In addition, it affects the levels of Bcl-2 pro- and anti-apoptotic proteins. However, it should be emphasized that the activity of resveratrol was specific for each of the tested cell lines, and the most statistically significant changes were observed in the mitoxantrone-resistant cells.Katarzyna RatajczakNatalia Glatzel-PlucińskaKatarzyna Ratajczak-WielgomasKatarzyna NowińskaSylwia BorskaMDPI AGarticleresveratrolBaxBcl-2Caspase-3apoptosismultidrug resistanceOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6560, p 6560 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resveratrol Bax Bcl-2 Caspase-3 apoptosis multidrug resistance Organic chemistry QD241-441
spellingShingle	resveratrol Bax Bcl-2 Caspase-3 apoptosis multidrug resistance Organic chemistry QD241-441 Katarzyna Ratajczak Natalia Glatzel-Plucińska Katarzyna Ratajczak-Wielgomas Katarzyna Nowińska Sylwia Borska Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
description	Pancreatic cancers are among of the most lethal types of neoplasms, and are mostly detected at an advanced stage. Conventional treatment methods such as chemotherapy or radiotherapy often do not bring the desired therapeutic effects. For this reason, natural compounds are increasingly being used as adjuvants in cancer therapy. Polyphenolic compounds, including resveratrol, are of particular interest. The aim of this study is to analyze the antiproliferative and pro-apoptotic mechanisms of resveratrol on human pancreatic cells. The study was carried out on three human pancreatic cancer cell lines: EPP85-181P, EPP85-181RNOV (mitoxantrone-resistant cells) and AsPC-1, as well as the normal pancreatic cell line H6c7. The cytotoxicity of resveratrol in the tested cell lines was assessed by the colorimetric method (MTT) and the flow cytometry method. Three selected concentrations of the compound (25, 50 and 100 µM) were tested in the experiments during a 48-h incubation. TUNEL and Comet assays, flow cytometry, immunocytochemistry, confocal microscopy, real-time PCR and Western Blot analyses were used to evaluate the pleiotropic effect of resveratrol. The results indicate that resveratrol is likely to be anticarcinogenic by inhibiting human pancreatic cancer cell proliferation. In addition, it affects the levels of Bcl-2 pro- and anti-apoptotic proteins. However, it should be emphasized that the activity of resveratrol was specific for each of the tested cell lines, and the most statistically significant changes were observed in the mitoxantrone-resistant cells.
format	article
author	Katarzyna Ratajczak Natalia Glatzel-Plucińska Katarzyna Ratajczak-Wielgomas Katarzyna Nowińska Sylwia Borska
author_facet	Katarzyna Ratajczak Natalia Glatzel-Plucińska Katarzyna Ratajczak-Wielgomas Katarzyna Nowińska Sylwia Borska
author_sort	Katarzyna Ratajczak
title	Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
title_short	Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
title_full	Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
title_fullStr	Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
title_full_unstemmed	Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members
title_sort	effect of resveratrol treatment on human pancreatic cancer cells through alterations of bcl-2 family members
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/148c960df9bc4ddeae858b2d1608b61e
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Effect of Resveratrol Treatment on Human Pancreatic Cancer Cells through Alterations of Bcl-2 Family Members

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