Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".

"Brachyspira hampsonii" causes disease indistinguishable from swine dysentery, and the structure of the intestinal microbiome likely plays a role in determining susceptibility of individual pigs to infection and development of clinical disease. The objectives of the current study were to d...

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Autores principales:	Matheus O Costa, Bonnie Chaban, John C S Harding, Janet E Hill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1490d2e7d0a945d3bd24262a5142bd87
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spelling	oai:doaj.org-article:1490d2e7d0a945d3bd24262a5142bd872021-11-25T06:02:49ZCharacterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".1932-620310.1371/journal.pone.0106399https://doaj.org/article/1490d2e7d0a945d3bd24262a5142bd872014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25166307/?tool=EBIhttps://doaj.org/toc/1932-6203"Brachyspira hampsonii" causes disease indistinguishable from swine dysentery, and the structure of the intestinal microbiome likely plays a role in determining susceptibility of individual pigs to infection and development of clinical disease. The objectives of the current study were to determine if the pre-inoculation fecal microbiota differed between inoculated pigs that did (INOC MH) or did not (INOC non-MH) develop mucohaemorrhagic diarrhea following challenge with "B. hampsonii", and to quantify changes in the structure of the microbiome following development of clinical disease. Fecal microbiota profiles were generated based on amplification and sequencing of the cpn60 universal target sequence from 89 samples from 18 pigs collected at -8, -5, -3 and 0 days post-inoculation, and at termination. No significant differences in richness, diversity or taxonomic composition distinguished the pre-inoculation microbiomes of INOC MH and INOC non-MH pigs. However, the development of bloody diarrhea in inoculated pigs was associated with perturbation of the microbiota relative to INOC non-MH or sham-inoculated control pigs. Specifically, the fecal microbiota of INOC MH pigs was less dense (fewer total 16S rRNA copies per gram of feces), and had a lower Bacteroidetes:Firmicutes ratio. Further investigation of the potential long-term effects of Brachyspira disease on intestinal health and performance is warranted.Matheus O CostaBonnie ChabanJohn C S HardingJanet E HillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e106399 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matheus O Costa Bonnie Chaban John C S Harding Janet E Hill Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
description	"Brachyspira hampsonii" causes disease indistinguishable from swine dysentery, and the structure of the intestinal microbiome likely plays a role in determining susceptibility of individual pigs to infection and development of clinical disease. The objectives of the current study were to determine if the pre-inoculation fecal microbiota differed between inoculated pigs that did (INOC MH) or did not (INOC non-MH) develop mucohaemorrhagic diarrhea following challenge with "B. hampsonii", and to quantify changes in the structure of the microbiome following development of clinical disease. Fecal microbiota profiles were generated based on amplification and sequencing of the cpn60 universal target sequence from 89 samples from 18 pigs collected at -8, -5, -3 and 0 days post-inoculation, and at termination. No significant differences in richness, diversity or taxonomic composition distinguished the pre-inoculation microbiomes of INOC MH and INOC non-MH pigs. However, the development of bloody diarrhea in inoculated pigs was associated with perturbation of the microbiota relative to INOC non-MH or sham-inoculated control pigs. Specifically, the fecal microbiota of INOC MH pigs was less dense (fewer total 16S rRNA copies per gram of feces), and had a lower Bacteroidetes:Firmicutes ratio. Further investigation of the potential long-term effects of Brachyspira disease on intestinal health and performance is warranted.
format	article
author	Matheus O Costa Bonnie Chaban John C S Harding Janet E Hill
author_facet	Matheus O Costa Bonnie Chaban John C S Harding Janet E Hill
author_sort	Matheus O Costa
title	Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
title_short	Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
title_full	Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
title_fullStr	Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
title_full_unstemmed	Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".
title_sort	characterization of the fecal microbiota of pigs before and after inoculation with "brachyspira hampsonii".
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1490d2e7d0a945d3bd24262a5142bd87
work_keys_str_mv	AT matheusocosta characterizationofthefecalmicrobiotaofpigsbeforeandafterinoculationwithbrachyspirahampsonii AT bonniechaban characterizationofthefecalmicrobiotaofpigsbeforeandafterinoculationwithbrachyspirahampsonii AT johncsharding characterizationofthefecalmicrobiotaofpigsbeforeandafterinoculationwithbrachyspirahampsonii AT janetehill characterizationofthefecalmicrobiotaofpigsbeforeandafterinoculationwithbrachyspirahampsonii
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Characterization of the fecal microbiota of pigs before and after inoculation with "Brachyspira hampsonii".

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