Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.

Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis worldwide and the molecular mechanism is not well understood. This study aimed to establish a collection of human HCC cell lines from patient-derived xenograft (PDX) models. From the 20 surgical HCC sample collections, 7 tumors we...

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Autores principales:	Hong Xin, Ke Wang, Gang Hu, Fubo Xie, Kedong Ouyang, Xuzhen Tang, Minjun Wang, Danyi Wen, Yizhun Zhu, Xiaoran Qin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1492cc0f37f1486984079837d2f53e2c
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spelling	oai:doaj.org-article:1492cc0f37f1486984079837d2f53e2c2021-11-18T08:38:09ZEstablishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.1932-620310.1371/journal.pone.0085308https://doaj.org/article/1492cc0f37f1486984079837d2f53e2c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416385/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis worldwide and the molecular mechanism is not well understood. This study aimed to establish a collection of human HCC cell lines from patient-derived xenograft (PDX) models. From the 20 surgical HCC sample collections, 7 tumors were successfully developed in immunodeficient mice and further established 7 novel HCC cell lines (LIXC002, LIXC003, LIXC004, LIXC006, LIXC011, LIXC012 and CPL0903) by primary culture. The characterization of cell lines was defined by morphology, growth kinetics, cell cycle, chromosome analysis, short tandem repeat (STR) analysis, molecular profile, and tumorigenicity. Additionally, response to clinical chemotherapeutics was validated both in vitro and in vivo. STR analysis indicated that all cell lines were unique cells different from known cell lines and free of contamination by bacteria or mycoplasma. The other findings were quite heterogeneous between individual lines. Chromosome aberration could be found in all cell lines. Alpha-fetoprotein was overexpressed only in 3 out of 7 cell lines. 4 cell lines expressed high level of vimentin. Ki67 was strongly stained in all cell lines. mRNA level of retinoic acid induced protein 3 (RAI3) was decreased in all cell lines. The 7 novel cell lines showed variable sensitivity to 8 tested compounds. LIXC011 and CPL0903 possessed multiple drug resistance property. Sorafenib inhibited xenograft tumor growth of LIXC006, but not of LIXC012. Our results indicated that the 7 novel cell lines with low passage maintaining their clinical and pathological characters could be good tools for further exploring the molecular mechanism of HCC and anti-cancer drug screening.Hong XinKe WangGang HuFubo XieKedong OuyangXuzhen TangMinjun WangDanyi WenYizhun ZhuXiaoran QinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85308 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong Xin Ke Wang Gang Hu Fubo Xie Kedong Ouyang Xuzhen Tang Minjun Wang Danyi Wen Yizhun Zhu Xiaoran Qin Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
description	Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis worldwide and the molecular mechanism is not well understood. This study aimed to establish a collection of human HCC cell lines from patient-derived xenograft (PDX) models. From the 20 surgical HCC sample collections, 7 tumors were successfully developed in immunodeficient mice and further established 7 novel HCC cell lines (LIXC002, LIXC003, LIXC004, LIXC006, LIXC011, LIXC012 and CPL0903) by primary culture. The characterization of cell lines was defined by morphology, growth kinetics, cell cycle, chromosome analysis, short tandem repeat (STR) analysis, molecular profile, and tumorigenicity. Additionally, response to clinical chemotherapeutics was validated both in vitro and in vivo. STR analysis indicated that all cell lines were unique cells different from known cell lines and free of contamination by bacteria or mycoplasma. The other findings were quite heterogeneous between individual lines. Chromosome aberration could be found in all cell lines. Alpha-fetoprotein was overexpressed only in 3 out of 7 cell lines. 4 cell lines expressed high level of vimentin. Ki67 was strongly stained in all cell lines. mRNA level of retinoic acid induced protein 3 (RAI3) was decreased in all cell lines. The 7 novel cell lines showed variable sensitivity to 8 tested compounds. LIXC011 and CPL0903 possessed multiple drug resistance property. Sorafenib inhibited xenograft tumor growth of LIXC006, but not of LIXC012. Our results indicated that the 7 novel cell lines with low passage maintaining their clinical and pathological characters could be good tools for further exploring the molecular mechanism of HCC and anti-cancer drug screening.
format	article
author	Hong Xin Ke Wang Gang Hu Fubo Xie Kedong Ouyang Xuzhen Tang Minjun Wang Danyi Wen Yizhun Zhu Xiaoran Qin
author_facet	Hong Xin Ke Wang Gang Hu Fubo Xie Kedong Ouyang Xuzhen Tang Minjun Wang Danyi Wen Yizhun Zhu Xiaoran Qin
author_sort	Hong Xin
title	Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
title_short	Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
title_full	Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
title_fullStr	Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
title_full_unstemmed	Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
title_sort	establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1492cc0f37f1486984079837d2f53e2c
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Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts.

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