The effect of gestational age on angiogenic gene expression in the rat placenta.

The placenta plays a central role in determining the outcome of pregnancy. It undergoes changes during gestation as the fetus develops and as demands for energy substrate transfer and gas exchange increase. The molecular mechanisms that coordinate these changes have yet to be fully elucidated. The s...

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Autores principales:	Kanchan Vaswani, Melissa Wen-Ching Hum, Hsiu-Wen Chan, Jennifer Ryan, Ryan J Wood-Bradley, Marloes Dekker Nitert, Murray D Mitchell, James A Armitage, Gregory E Rice
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/149499f543bd4c268f1511993d6d6e0b
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spelling	oai:doaj.org-article:149499f543bd4c268f1511993d6d6e0b2021-11-18T08:39:31ZThe effect of gestational age on angiogenic gene expression in the rat placenta.1932-620310.1371/journal.pone.0083762https://doaj.org/article/149499f543bd4c268f1511993d6d6e0b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391823/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The placenta plays a central role in determining the outcome of pregnancy. It undergoes changes during gestation as the fetus develops and as demands for energy substrate transfer and gas exchange increase. The molecular mechanisms that coordinate these changes have yet to be fully elucidated. The study performed a large scale screen of the transcriptome of the rat placenta throughout mid-late gestation (E14.25-E20) with emphasis on characterizing gestational age associated changes in the expression of genes involved in angiogenic pathways. Sprague Dawley dams were sacrificed at E14.25, E15.25, E17.25 and E20 (n = 6 per group) and RNA was isolated from one placenta per dam. Changes in placental gene expression were identified using Illumina Rat Ref-12 Expression BeadChip Microarrays. Differentially expressed genes (>2-fold change, <1% false discovery rate, FDR) were functionally categorised by gene ontology pathway analysis. A subset of differentially expressed genes identified by microarrays were confirmed using Real-Time qPCR. The expression of thirty one genes involved in the angiogenic pathway was shown to change over time, using microarray analysis (22 genes displayed increased and 9 gene decreased expression). Five genes (4 up regulated: Cd36, Mmp14, Rhob and Angpt4 and 1 down regulated: Foxm1) involved in angiogenesis and blood vessel morphogenesis were subjected to further validation. qPCR confirmed late gestational increased expression of Cd36, Mmp14, Rhob and Angpt4 and a decrease in expression of Foxm1 before labour onset (P<0.0001). The observed acute, pre-labour changes in the expression of the 31 genes during gestation warrant further investigation to elucidate their role in pregnancy.Kanchan VaswaniMelissa Wen-Ching HumHsiu-Wen ChanJennifer RyanRyan J Wood-BradleyMarloes Dekker NitertMurray D MitchellJames A ArmitageGregory E RicePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83762 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kanchan Vaswani Melissa Wen-Ching Hum Hsiu-Wen Chan Jennifer Ryan Ryan J Wood-Bradley Marloes Dekker Nitert Murray D Mitchell James A Armitage Gregory E Rice The effect of gestational age on angiogenic gene expression in the rat placenta.
description	The placenta plays a central role in determining the outcome of pregnancy. It undergoes changes during gestation as the fetus develops and as demands for energy substrate transfer and gas exchange increase. The molecular mechanisms that coordinate these changes have yet to be fully elucidated. The study performed a large scale screen of the transcriptome of the rat placenta throughout mid-late gestation (E14.25-E20) with emphasis on characterizing gestational age associated changes in the expression of genes involved in angiogenic pathways. Sprague Dawley dams were sacrificed at E14.25, E15.25, E17.25 and E20 (n = 6 per group) and RNA was isolated from one placenta per dam. Changes in placental gene expression were identified using Illumina Rat Ref-12 Expression BeadChip Microarrays. Differentially expressed genes (>2-fold change, <1% false discovery rate, FDR) were functionally categorised by gene ontology pathway analysis. A subset of differentially expressed genes identified by microarrays were confirmed using Real-Time qPCR. The expression of thirty one genes involved in the angiogenic pathway was shown to change over time, using microarray analysis (22 genes displayed increased and 9 gene decreased expression). Five genes (4 up regulated: Cd36, Mmp14, Rhob and Angpt4 and 1 down regulated: Foxm1) involved in angiogenesis and blood vessel morphogenesis were subjected to further validation. qPCR confirmed late gestational increased expression of Cd36, Mmp14, Rhob and Angpt4 and a decrease in expression of Foxm1 before labour onset (P<0.0001). The observed acute, pre-labour changes in the expression of the 31 genes during gestation warrant further investigation to elucidate their role in pregnancy.
format	article
author	Kanchan Vaswani Melissa Wen-Ching Hum Hsiu-Wen Chan Jennifer Ryan Ryan J Wood-Bradley Marloes Dekker Nitert Murray D Mitchell James A Armitage Gregory E Rice
author_facet	Kanchan Vaswani Melissa Wen-Ching Hum Hsiu-Wen Chan Jennifer Ryan Ryan J Wood-Bradley Marloes Dekker Nitert Murray D Mitchell James A Armitage Gregory E Rice
author_sort	Kanchan Vaswani
title	The effect of gestational age on angiogenic gene expression in the rat placenta.
title_short	The effect of gestational age on angiogenic gene expression in the rat placenta.
title_full	The effect of gestational age on angiogenic gene expression in the rat placenta.
title_fullStr	The effect of gestational age on angiogenic gene expression in the rat placenta.
title_full_unstemmed	The effect of gestational age on angiogenic gene expression in the rat placenta.
title_sort	effect of gestational age on angiogenic gene expression in the rat placenta.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/149499f543bd4c268f1511993d6d6e0b
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The effect of gestational age on angiogenic gene expression in the rat placenta.

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