Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli

Abstract The dissemination of carbapenem resistance in Escherichia coli has major implications for the management of common infections. bla KPC, encoding a transmissible carbapenemase (KPC), has historically largely been associated with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a sp...

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Autores principales:	N. Stoesser, A. E. Sheppard, G. Peirano, L. W. Anson, L. Pankhurst, R. Sebra, H. T. T. Phan, A. Kasarskis, A. J. Mathers, T. E. A. Peto, P. Bradford, M. R. Motyl, A. S. Walker, D. W. Crook, J. D. Pitout
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/149ae8286e534a248080c7454a854804
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spelling	oai:doaj.org-article:149ae8286e534a248080c7454a8548042021-12-02T11:52:30ZGenomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli10.1038/s41598-017-06256-22045-2322https://doaj.org/article/149ae8286e534a248080c7454a8548042017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06256-2https://doaj.org/toc/2045-2322Abstract The dissemination of carbapenem resistance in Escherichia coli has major implications for the management of common infections. bla KPC, encoding a transmissible carbapenemase (KPC), has historically largely been associated with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a specific transposable element (Tn4401, ~10 kb). Here we characterize the genetic features of bla KPC emergence in global E. coli, 2008–2013, using both long- and short-read whole-genome sequencing. Amongst 43/45 successfully sequenced bla KPC-E. coli strains, we identified substantial strain diversity (n = 21 sequence types, 18% of annotated genes in the core genome); substantial plasmid diversity (≥9 replicon types); and substantial bla KPC-associated, mobile genetic element (MGE) diversity (50% not within complete Tn4401 elements). We also found evidence of inter-species, regional and international plasmid spread. In several cases bla KPC was found on high copy number, small Col-like plasmids, previously associated with horizontal transmission of resistance genes in the absence of antimicrobial selection pressures. E. coli is a common human pathogen, but also a commensal in multiple environmental and animal reservoirs, and easily transmissible. The association of bla KPC with a range of MGEs previously linked to the successful spread of widely endemic resistance mechanisms (e.g. bla TEM, bla CTX-M) suggests that it may become similarly prevalent.N. StoesserA. E. SheppardG. PeiranoL. W. AnsonL. PankhurstR. SebraH. T. T. PhanA. KasarskisA. J. MathersT. E. A. PetoP. BradfordM. R. MotylA. S. WalkerD. W. CrookJ. D. PitoutNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q N. Stoesser A. E. Sheppard G. Peirano L. W. Anson L. Pankhurst R. Sebra H. T. T. Phan A. Kasarskis A. J. Mathers T. E. A. Peto P. Bradford M. R. Motyl A. S. Walker D. W. Crook J. D. Pitout Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
description	Abstract The dissemination of carbapenem resistance in Escherichia coli has major implications for the management of common infections. bla KPC, encoding a transmissible carbapenemase (KPC), has historically largely been associated with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a specific transposable element (Tn4401, ~10 kb). Here we characterize the genetic features of bla KPC emergence in global E. coli, 2008–2013, using both long- and short-read whole-genome sequencing. Amongst 43/45 successfully sequenced bla KPC-E. coli strains, we identified substantial strain diversity (n = 21 sequence types, 18% of annotated genes in the core genome); substantial plasmid diversity (≥9 replicon types); and substantial bla KPC-associated, mobile genetic element (MGE) diversity (50% not within complete Tn4401 elements). We also found evidence of inter-species, regional and international plasmid spread. In several cases bla KPC was found on high copy number, small Col-like plasmids, previously associated with horizontal transmission of resistance genes in the absence of antimicrobial selection pressures. E. coli is a common human pathogen, but also a commensal in multiple environmental and animal reservoirs, and easily transmissible. The association of bla KPC with a range of MGEs previously linked to the successful spread of widely endemic resistance mechanisms (e.g. bla TEM, bla CTX-M) suggests that it may become similarly prevalent.
format	article
author	N. Stoesser A. E. Sheppard G. Peirano L. W. Anson L. Pankhurst R. Sebra H. T. T. Phan A. Kasarskis A. J. Mathers T. E. A. Peto P. Bradford M. R. Motyl A. S. Walker D. W. Crook J. D. Pitout
author_facet	N. Stoesser A. E. Sheppard G. Peirano L. W. Anson L. Pankhurst R. Sebra H. T. T. Phan A. Kasarskis A. J. Mathers T. E. A. Peto P. Bradford M. R. Motyl A. S. Walker D. W. Crook J. D. Pitout
author_sort	N. Stoesser
title	Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
title_short	Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
title_full	Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
title_fullStr	Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
title_full_unstemmed	Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli
title_sort	genomic epidemiology of global klebsiella pneumoniae carbapenemase (kpc)-producing escherichia coli
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/149ae8286e534a248080c7454a854804
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Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli

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