Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs

Somatosensory stimuli guide and shape behavior, from immediate protective reflexes to longer-term learning and higher-order processes related to pain and touch. However, somatosensory inputs are challenging to control in awake mammals due to the diversity and nature of contact stimuli. Application o...

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Autores principales:	Ara Schorscher-Petcu, Flóra Takács, Liam E Browne
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	somatosensation pain touch machine vision behavior optogenetics Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/149b30ce43e547b487c840a571072f1d
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spelling	oai:doaj.org-article:149b30ce43e547b487c840a571072f1d2021-11-24T12:23:51ZScanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs10.7554/eLife.620262050-084Xe62026https://doaj.org/article/149b30ce43e547b487c840a571072f1d2021-07-01T00:00:00Zhttps://elifesciences.org/articles/62026https://doaj.org/toc/2050-084XSomatosensory stimuli guide and shape behavior, from immediate protective reflexes to longer-term learning and higher-order processes related to pain and touch. However, somatosensory inputs are challenging to control in awake mammals due to the diversity and nature of contact stimuli. Application of cutaneous stimuli is currently limited to relatively imprecise methods as well as subjective behavioral measures. The strategy we present here overcomes these difficulties, achieving ‘remote touch’ with spatiotemporally precise and dynamic optogenetic stimulation by projecting light to a small defined area of skin. We mapped behavioral responses in freely behaving mice with specific nociceptor and low-threshold mechanoreceptor inputs. In nociceptors, sparse recruitment of single-action potentials shapes rapid protective pain-related behaviors, including coordinated head orientation and body repositioning that depend on the initial body pose. In contrast, activation of low-threshold mechanoreceptors elicited slow-onset behaviors and more subtle whole-body behaviors. The strategy can be used to define specific behavioral repertoires, examine the timing and nature of reflexes, and dissect sensory, motor, cognitive, and motivational processes guiding behavior.Ara Schorscher-PetcuFlóra TakácsLiam E BrowneeLife Sciences Publications Ltdarticlesomatosensationpaintouchmachine visionbehavioroptogeneticsMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	somatosensation pain touch machine vision behavior optogenetics Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	somatosensation pain touch machine vision behavior optogenetics Medicine R Science Q Biology (General) QH301-705.5 Ara Schorscher-Petcu Flóra Takács Liam E Browne Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
description	Somatosensory stimuli guide and shape behavior, from immediate protective reflexes to longer-term learning and higher-order processes related to pain and touch. However, somatosensory inputs are challenging to control in awake mammals due to the diversity and nature of contact stimuli. Application of cutaneous stimuli is currently limited to relatively imprecise methods as well as subjective behavioral measures. The strategy we present here overcomes these difficulties, achieving ‘remote touch’ with spatiotemporally precise and dynamic optogenetic stimulation by projecting light to a small defined area of skin. We mapped behavioral responses in freely behaving mice with specific nociceptor and low-threshold mechanoreceptor inputs. In nociceptors, sparse recruitment of single-action potentials shapes rapid protective pain-related behaviors, including coordinated head orientation and body repositioning that depend on the initial body pose. In contrast, activation of low-threshold mechanoreceptors elicited slow-onset behaviors and more subtle whole-body behaviors. The strategy can be used to define specific behavioral repertoires, examine the timing and nature of reflexes, and dissect sensory, motor, cognitive, and motivational processes guiding behavior.
format	article
author	Ara Schorscher-Petcu Flóra Takács Liam E Browne
author_facet	Ara Schorscher-Petcu Flóra Takács Liam E Browne
author_sort	Ara Schorscher-Petcu
title	Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
title_short	Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
title_full	Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
title_fullStr	Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
title_full_unstemmed	Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
title_sort	scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/149b30ce43e547b487c840a571072f1d
work_keys_str_mv	AT araschorscherpetcu scannedoptogeneticcontrolofmammaliansomatosensoryinputtomapinputspecificbehavioraloutputs AT floratakacs scannedoptogeneticcontrolofmammaliansomatosensoryinputtomapinputspecificbehavioraloutputs AT liamebrowne scannedoptogeneticcontrolofmammaliansomatosensoryinputtomapinputspecificbehavioraloutputs
_version_	1718415032150130688

Scanned optogenetic control of mammalian somatosensory input to map input-specific behavioral outputs

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