The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia

Abstract The aim of this study is to establish the expression of ATP-sensitive potassium channels(KATP) in human umbilical arteries with severe pre-eclampsia. Real-time quantitative PCR and western blotting were used to detect the mRNA and protein expression levels of KATP channel subunits Kir6.1 an...

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Autores principales:	Benlan Yin, Yujiao Zhang, Xiaohong Wei, Chunrong Pang, Ting Hou, Chao Yang, Yuzhi Ning, Xiaodong Fu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/149d0e06d9a747e5816aa1576fcc2299
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spelling	oai:doaj.org-article:149d0e06d9a747e5816aa1576fcc22992021-12-02T15:51:16ZThe expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia10.1038/s41598-021-87146-62045-2322https://doaj.org/article/149d0e06d9a747e5816aa1576fcc22992021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87146-6https://doaj.org/toc/2045-2322Abstract The aim of this study is to establish the expression of ATP-sensitive potassium channels(KATP) in human umbilical arteries with severe pre-eclampsia. Real-time quantitative PCR and western blotting were used to detect the mRNA and protein expression levels of KATP channel subunits Kir6.1 and SUR2B in human umbilical arteries from normal pregnant and those with severe pre-eclampsia, early onset severe pre-eclampsia and late onset severe pre-eclampsia. The mRNA and protein levels of SUR2B in the severe pre-eclampsia group were lower than those in the normal group (P < 0.001), and the expression of Kir6.1 was not statistically significant between the two groups (P > 0.05). The mRNA and protein levels of SUR2B in early onset severe pre-eclampsia group were lower than those in late onset severe pre-eclampsia group (P < 0.001). There was no significant difference in expression of Kir6.1 between the two groups (P > 0.05). The mRNA and protein expression levels of SUR2B in pregnant women with severe pre-eclampsia were lower than those in normal pregnant women, suggesting that the expression of the SUR2B of the KATP channel may be related to the occurrence and development of severe pre-eclampsia. Compared with late onset severe pre-eclampsia, the mRNA and protein expression levels of SUR2B were lower in the umbilical arteries of women with early onset severe pre-eclampsia, suggesting that the occurrence time of severe pre-eclampsia may be related to the extent reduced expression of the SUR2B of the KATP channel.Benlan YinYujiao ZhangXiaohong WeiChunrong PangTing HouChao YangYuzhi NingXiaodong FuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benlan Yin Yujiao Zhang Xiaohong Wei Chunrong Pang Ting Hou Chao Yang Yuzhi Ning Xiaodong Fu The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
description	Abstract The aim of this study is to establish the expression of ATP-sensitive potassium channels(KATP) in human umbilical arteries with severe pre-eclampsia. Real-time quantitative PCR and western blotting were used to detect the mRNA and protein expression levels of KATP channel subunits Kir6.1 and SUR2B in human umbilical arteries from normal pregnant and those with severe pre-eclampsia, early onset severe pre-eclampsia and late onset severe pre-eclampsia. The mRNA and protein levels of SUR2B in the severe pre-eclampsia group were lower than those in the normal group (P < 0.001), and the expression of Kir6.1 was not statistically significant between the two groups (P > 0.05). The mRNA and protein levels of SUR2B in early onset severe pre-eclampsia group were lower than those in late onset severe pre-eclampsia group (P < 0.001). There was no significant difference in expression of Kir6.1 between the two groups (P > 0.05). The mRNA and protein expression levels of SUR2B in pregnant women with severe pre-eclampsia were lower than those in normal pregnant women, suggesting that the expression of the SUR2B of the KATP channel may be related to the occurrence and development of severe pre-eclampsia. Compared with late onset severe pre-eclampsia, the mRNA and protein expression levels of SUR2B were lower in the umbilical arteries of women with early onset severe pre-eclampsia, suggesting that the occurrence time of severe pre-eclampsia may be related to the extent reduced expression of the SUR2B of the KATP channel.
format	article
author	Benlan Yin Yujiao Zhang Xiaohong Wei Chunrong Pang Ting Hou Chao Yang Yuzhi Ning Xiaodong Fu
author_facet	Benlan Yin Yujiao Zhang Xiaohong Wei Chunrong Pang Ting Hou Chao Yang Yuzhi Ning Xiaodong Fu
author_sort	Benlan Yin
title	The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
title_short	The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
title_full	The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
title_fullStr	The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
title_full_unstemmed	The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
title_sort	expression of atp-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/149d0e06d9a747e5816aa1576fcc2299
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The expression of ATP-sensitive potassium channels in human umbilical arteries with severe pre-eclampsia

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