Defining the expression of marker genes in equine mesenchymal stromal cells

Deborah J Guest1, Jennifer C Ousey1, Matthew RW Smith21Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU; 2Reynolds House Referrals, Greenwood Ellis and Partners, 166 High Street, Newmarket, Suffolk, CB8 9WS, UKAbstract: Mesenchymal stromal (MS) cells have been derived from m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Deborah J Guest, Jennifer C Ousey, Matthew RW Smith
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/149e4d420a2e4d8f9b95eff31c8ea86c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:149e4d420a2e4d8f9b95eff31c8ea86c
record_format	dspace
spelling	oai:doaj.org-article:149e4d420a2e4d8f9b95eff31c8ea86c2021-12-02T00:23:49ZDefining the expression of marker genes in equine mesenchymal stromal cells1178-6957https://doaj.org/article/149e4d420a2e4d8f9b95eff31c8ea86c2008-11-01T00:00:00Zhttp://www.dovepress.com/defining-the-expression-of-marker-genes-in-equine-mesenchymal-stromal--a2539https://doaj.org/toc/1178-6957Deborah J Guest1, Jennifer C Ousey1, Matthew RW Smith21Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU; 2Reynolds House Referrals, Greenwood Ellis and Partners, 166 High Street, Newmarket, Suffolk, CB8 9WS, UKAbstract: Mesenchymal stromal (MS) cells have been derived from multiple sources in the horse including bone marrow, adipose tissue and umbilical cord blood. To date these cells have been investigated for their differentiation potential and are currently being used to treat damage to horse musculoskeletal tissues. However, no work has been done in horse MS cells to examine the expression profile of proteins and cell surface antigens that are expressed in human MS cells. The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from different laboratories and different tissues. At present it is difficult to ascertain whether equivalent cells are being used in different reports. Here, we report on the expression of a range of markers used to define human MS cells. Using immunocytochemistry we show that horse MS cells homogenously express collagens, alkaline phosphatase activity, CD44, CD90 and CD29. In contrast, CD14, CD79&alpha; and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen) -1, -3, -4, TRA (tumor rejection antigen) -1&ndash;60 and -1&ndash;81 are not expressed. The MS cells also express MHC class I antigens but do not express class II antigens, although they are inducible by treatment with interferon gamma (IFN-&gamma;).Keywords: mesenchymal stem cells, equine, gene expression Deborah J GuestJennifer C OuseyMatthew RW SmithDove Medical PressarticleCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2008, Iss default, Pp 1-9 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Deborah J Guest Jennifer C Ousey Matthew RW Smith Defining the expression of marker genes in equine mesenchymal stromal cells
description	Deborah J Guest1, Jennifer C Ousey1, Matthew RW Smith21Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU; 2Reynolds House Referrals, Greenwood Ellis and Partners, 166 High Street, Newmarket, Suffolk, CB8 9WS, UKAbstract: Mesenchymal stromal (MS) cells have been derived from multiple sources in the horse including bone marrow, adipose tissue and umbilical cord blood. To date these cells have been investigated for their differentiation potential and are currently being used to treat damage to horse musculoskeletal tissues. However, no work has been done in horse MS cells to examine the expression profile of proteins and cell surface antigens that are expressed in human MS cells. The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from different laboratories and different tissues. At present it is difficult to ascertain whether equivalent cells are being used in different reports. Here, we report on the expression of a range of markers used to define human MS cells. Using immunocytochemistry we show that horse MS cells homogenously express collagens, alkaline phosphatase activity, CD44, CD90 and CD29. In contrast, CD14, CD79&alpha; and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen) -1, -3, -4, TRA (tumor rejection antigen) -1&ndash;60 and -1&ndash;81 are not expressed. The MS cells also express MHC class I antigens but do not express class II antigens, although they are inducible by treatment with interferon gamma (IFN-&gamma;).Keywords: mesenchymal stem cells, equine, gene expression
format	article
author	Deborah J Guest Jennifer C Ousey Matthew RW Smith
author_facet	Deborah J Guest Jennifer C Ousey Matthew RW Smith
author_sort	Deborah J Guest
title	Defining the expression of marker genes in equine mesenchymal stromal cells
title_short	Defining the expression of marker genes in equine mesenchymal stromal cells
title_full	Defining the expression of marker genes in equine mesenchymal stromal cells
title_fullStr	Defining the expression of marker genes in equine mesenchymal stromal cells
title_full_unstemmed	Defining the expression of marker genes in equine mesenchymal stromal cells
title_sort	defining the expression of marker genes in equine mesenchymal stromal cells
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/149e4d420a2e4d8f9b95eff31c8ea86c
work_keys_str_mv	AT deborahjguest definingtheexpressionofmarkergenesinequinemesenchymalstromalcells AT jennifercousey definingtheexpressionofmarkergenesinequinemesenchymalstromalcells AT matthewrwsmith definingtheexpressionofmarkergenesinequinemesenchymalstromalcells
_version_	1718403724390432768

Defining the expression of marker genes in equine mesenchymal stromal cells

Ejemplares similares