Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine

Abstract Inactivated poultry vaccines are subject to routine potency testing for batch release, requiring large numbers of animals. The replacement of in vivo tests for cell-based alternatives can be facilitated by the identification of biomarkers for vaccine-induced immune responses. In this study,...

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Autores principales:	Robin H. G. A. van den Biggelaar, Larissa van der Maas, Hugo D. Meiring, Jeroen L. A. Pennings, Willem van Eden, Victor P. M. G. Rutten, Christine A. Jansen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14a48ada534443fc9c1e168d1b014e78
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spelling	oai:doaj.org-article:14a48ada534443fc9c1e168d1b014e782021-12-02T17:41:26ZProteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine10.1038/s41598-021-89810-32045-2322https://doaj.org/article/14a48ada534443fc9c1e168d1b014e782021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89810-3https://doaj.org/toc/2045-2322Abstract Inactivated poultry vaccines are subject to routine potency testing for batch release, requiring large numbers of animals. The replacement of in vivo tests for cell-based alternatives can be facilitated by the identification of biomarkers for vaccine-induced immune responses. In this study, chicken bone marrow-derived dendritic cells were stimulated with an inactivated vaccine for infectious bronchitis virus and Newcastle disease virus, as well as inactivated infectious bronchitis virus only, and lipopolysaccharides as positive control, or left unstimulated for comparison with the stimulated samples. Next, the cells were lysed and subjected to proteomic analysis. Stimulation with the vaccine resulted in 66 differentially expressed proteins associated with mRNA translation, immune responses, lipid metabolism and the proteasome. For the eight most significantly upregulated proteins, mRNA expression levels were assessed. Markers that showed increased expression at both mRNA and protein levels included PLIN2 and PSMB1. Stimulation with infectious bronchitis virus only resulted in 25 differentially expressed proteins, which were mostly proteins containing Src homology 2 domains. Stimulation with lipopolysaccharides resulted in 118 differentially expressed proteins associated with dendritic cell maturation and antimicrobial activity. This study provides leads to a better understanding of the activation of dendritic cells by an inactivated poultry vaccine, and identified PLIN2 and PSMB1 as potential biomarkers for cell-based potency testing.Robin H. G. A. van den BiggelaarLarissa van der MaasHugo D. MeiringJeroen L. A. PenningsWillem van EdenVictor P. M. G. RuttenChristine A. JansenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robin H. G. A. van den Biggelaar Larissa van der Maas Hugo D. Meiring Jeroen L. A. Pennings Willem van Eden Victor P. M. G. Rutten Christine A. Jansen Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
description	Abstract Inactivated poultry vaccines are subject to routine potency testing for batch release, requiring large numbers of animals. The replacement of in vivo tests for cell-based alternatives can be facilitated by the identification of biomarkers for vaccine-induced immune responses. In this study, chicken bone marrow-derived dendritic cells were stimulated with an inactivated vaccine for infectious bronchitis virus and Newcastle disease virus, as well as inactivated infectious bronchitis virus only, and lipopolysaccharides as positive control, or left unstimulated for comparison with the stimulated samples. Next, the cells were lysed and subjected to proteomic analysis. Stimulation with the vaccine resulted in 66 differentially expressed proteins associated with mRNA translation, immune responses, lipid metabolism and the proteasome. For the eight most significantly upregulated proteins, mRNA expression levels were assessed. Markers that showed increased expression at both mRNA and protein levels included PLIN2 and PSMB1. Stimulation with infectious bronchitis virus only resulted in 25 differentially expressed proteins, which were mostly proteins containing Src homology 2 domains. Stimulation with lipopolysaccharides resulted in 118 differentially expressed proteins associated with dendritic cell maturation and antimicrobial activity. This study provides leads to a better understanding of the activation of dendritic cells by an inactivated poultry vaccine, and identified PLIN2 and PSMB1 as potential biomarkers for cell-based potency testing.
format	article
author	Robin H. G. A. van den Biggelaar Larissa van der Maas Hugo D. Meiring Jeroen L. A. Pennings Willem van Eden Victor P. M. G. Rutten Christine A. Jansen
author_facet	Robin H. G. A. van den Biggelaar Larissa van der Maas Hugo D. Meiring Jeroen L. A. Pennings Willem van Eden Victor P. M. G. Rutten Christine A. Jansen
author_sort	Robin H. G. A. van den Biggelaar
title	Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
title_short	Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
title_full	Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
title_fullStr	Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
title_full_unstemmed	Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine
title_sort	proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated ibv + ndv poultry vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/14a48ada534443fc9c1e168d1b014e78
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Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine

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