A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy

(1) Background: Left ventricular hypertrophy, myocardial disarray and interstitial fibrosis are the hallmarks of hypertrophic cardiomyopathy (HCM). Access to the myocardium for diagnostic purposes is limited. Circulating biomolecules reflecting the myocardial disease processes could improve the earl...

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Autores principales:	Tilo Thottakara, Natalie Lund, Elisabeth Krämer, Paulus Kirchhof, Lucie Carrier, Monica Patten
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	circulating miRNA biomarker ventricular hypertrophy hypertrophic cardiomyopathy cardiac fibrosis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/14ab195569af493da3d53124a54132a9
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spelling	oai:doaj.org-article:14ab195569af493da3d53124a54132a92021-11-25T16:54:16ZA Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy10.3390/biom111117182218-273Xhttps://doaj.org/article/14ab195569af493da3d53124a54132a92021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1718https://doaj.org/toc/2218-273X(1) Background: Left ventricular hypertrophy, myocardial disarray and interstitial fibrosis are the hallmarks of hypertrophic cardiomyopathy (HCM). Access to the myocardium for diagnostic purposes is limited. Circulating biomolecules reflecting the myocardial disease processes could improve the early detection of HCM. Circulating miRNAs have been found to reflect disease processes in several cardiovascular diseases. (2) Methods: We quantified circulating miRNA molecules in the plasma of 24 HCM and 11 healthy controls using the Human v3 miRNA Expression Assay Kit Code set (Nanostring Tech., Seattle, WA, USA) and validated differentially expressed miRNAs using RT-PCR. (3) Results: In comparison to healthy controls, the levels of six miRNAs (miR-1, miR-3144, miR-4454, miR-495-3p, miR-499a-5p and miR-627-3p) were higher in the plasma of HCM patients than healthy individuals (<i>p</i> < 0.05). Of these, higher levels of miR-1, miR-495 and miR-4454 could be validated by real-time PCR. In addition, elevated miR-4454 levels were significantly correlated with cardiac fibrosis, detected by magnetic resonance imaging in HCM patients. (4) Conclusions: Circulating miR-1, miR-495-3p and miR-4454 levels are elevated in the plasma of HCM patients. To the best of our knowledge, this is the first report showing a correlation between miR-4454 levels and cardiac fibrosis in HCM. This suggests miR-4454 as a potential biomarker for fibrosis in these patients.Tilo ThottakaraNatalie LundElisabeth KrämerPaulus KirchhofLucie CarrierMonica PattenMDPI AGarticlecirculating miRNAbiomarkerventricular hypertrophyhypertrophic cardiomyopathycardiac fibrosisMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1718, p 1718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circulating miRNA biomarker ventricular hypertrophy hypertrophic cardiomyopathy cardiac fibrosis Microbiology QR1-502
spellingShingle	circulating miRNA biomarker ventricular hypertrophy hypertrophic cardiomyopathy cardiac fibrosis Microbiology QR1-502 Tilo Thottakara Natalie Lund Elisabeth Krämer Paulus Kirchhof Lucie Carrier Monica Patten A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
description	(1) Background: Left ventricular hypertrophy, myocardial disarray and interstitial fibrosis are the hallmarks of hypertrophic cardiomyopathy (HCM). Access to the myocardium for diagnostic purposes is limited. Circulating biomolecules reflecting the myocardial disease processes could improve the early detection of HCM. Circulating miRNAs have been found to reflect disease processes in several cardiovascular diseases. (2) Methods: We quantified circulating miRNA molecules in the plasma of 24 HCM and 11 healthy controls using the Human v3 miRNA Expression Assay Kit Code set (Nanostring Tech., Seattle, WA, USA) and validated differentially expressed miRNAs using RT-PCR. (3) Results: In comparison to healthy controls, the levels of six miRNAs (miR-1, miR-3144, miR-4454, miR-495-3p, miR-499a-5p and miR-627-3p) were higher in the plasma of HCM patients than healthy individuals (<i>p</i> < 0.05). Of these, higher levels of miR-1, miR-495 and miR-4454 could be validated by real-time PCR. In addition, elevated miR-4454 levels were significantly correlated with cardiac fibrosis, detected by magnetic resonance imaging in HCM patients. (4) Conclusions: Circulating miR-1, miR-495-3p and miR-4454 levels are elevated in the plasma of HCM patients. To the best of our knowledge, this is the first report showing a correlation between miR-4454 levels and cardiac fibrosis in HCM. This suggests miR-4454 as a potential biomarker for fibrosis in these patients.
format	article
author	Tilo Thottakara Natalie Lund Elisabeth Krämer Paulus Kirchhof Lucie Carrier Monica Patten
author_facet	Tilo Thottakara Natalie Lund Elisabeth Krämer Paulus Kirchhof Lucie Carrier Monica Patten
author_sort	Tilo Thottakara
title	A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
title_short	A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
title_full	A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
title_fullStr	A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
title_full_unstemmed	A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy
title_sort	novel mirna screen identifies mirna-4454 as a candidate biomarker for ventricular fibrosis in patients with hypertrophic cardiomyopathy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/14ab195569af493da3d53124a54132a9
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A Novel miRNA Screen Identifies miRNA-4454 as a Candidate Biomarker for Ventricular Fibrosis in Patients with Hypertrophic Cardiomyopathy

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