Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction

Nuclear autophagy is an important selective autophagy process. The selective autophagy of sexual development micronuclei (MICs) and the programmed nuclear degradation of parental macronucleus (paMAC) occur during sexual reproduction in <i>Tetrahymena thermophila</i>. The molecular regula...

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Autores principales:	Tao Bo, Yu Kang, Ya Liu, Jing Xu, Wei Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	autophagy-related protein 5 parental macronucleus programmed nuclear degradation <i>Tetrahymena thermophila</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/14b3ecd090a341f2a3691aa624da9b26
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spelling	oai:doaj.org-article:14b3ecd090a341f2a3691aa624da9b262021-11-25T17:11:10ZAtg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction10.3390/cells101130712073-4409https://doaj.org/article/14b3ecd090a341f2a3691aa624da9b262021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3071https://doaj.org/toc/2073-4409Nuclear autophagy is an important selective autophagy process. The selective autophagy of sexual development micronuclei (MICs) and the programmed nuclear degradation of parental macronucleus (paMAC) occur during sexual reproduction in <i>Tetrahymena thermophila</i>. The molecular regulatory mechanism of nuclear selective autophagy is unclear. In this study, the autophagy-related protein Atg5 was identified from <i>T. thermophila</i>. Atg5 was localized in the cytoplasm in the early sexual-development stage and was localized in the paMAC in the late sexual-development stage. During this stage, the degradation of meiotic products of MIC was delayed in <i>atg5i</i> mutants. Furthermore, paMAC was abnormally enlarged and delayed or failed to degrade. The expression level and lipidation of Atg8.2 significantly decreased in the mutants. All these results indicated that Atg5 was involved in the regulation of the selective autophagy of paMAC by regulating Atg8.2 in <i>Tetrahymena.</i>Tao BoYu KangYa LiuJing XuWei WangMDPI AGarticleautophagy-related protein 5parental macronucleusprogrammed nuclear degradation<i>Tetrahymena thermophila</i>Biology (General)QH301-705.5ENCells, Vol 10, Iss 3071, p 3071 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autophagy-related protein 5 parental macronucleus programmed nuclear degradation <i>Tetrahymena thermophila</i> Biology (General) QH301-705.5
spellingShingle	autophagy-related protein 5 parental macronucleus programmed nuclear degradation <i>Tetrahymena thermophila</i> Biology (General) QH301-705.5 Tao Bo Yu Kang Ya Liu Jing Xu Wei Wang Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
description	Nuclear autophagy is an important selective autophagy process. The selective autophagy of sexual development micronuclei (MICs) and the programmed nuclear degradation of parental macronucleus (paMAC) occur during sexual reproduction in <i>Tetrahymena thermophila</i>. The molecular regulatory mechanism of nuclear selective autophagy is unclear. In this study, the autophagy-related protein Atg5 was identified from <i>T. thermophila</i>. Atg5 was localized in the cytoplasm in the early sexual-development stage and was localized in the paMAC in the late sexual-development stage. During this stage, the degradation of meiotic products of MIC was delayed in <i>atg5i</i> mutants. Furthermore, paMAC was abnormally enlarged and delayed or failed to degrade. The expression level and lipidation of Atg8.2 significantly decreased in the mutants. All these results indicated that Atg5 was involved in the regulation of the selective autophagy of paMAC by regulating Atg8.2 in <i>Tetrahymena.</i>
format	article
author	Tao Bo Yu Kang Ya Liu Jing Xu Wei Wang
author_facet	Tao Bo Yu Kang Ya Liu Jing Xu Wei Wang
author_sort	Tao Bo
title	Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
title_short	Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
title_full	Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
title_fullStr	Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
title_full_unstemmed	Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction
title_sort	atg5 regulates selective autophagy of the parental macronucleus during <i>tetrahymena</i> sexual reproduction
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/14b3ecd090a341f2a3691aa624da9b26
work_keys_str_mv	AT taobo atg5regulatesselectiveautophagyoftheparentalmacronucleusduringitetrahymenaisexualreproduction AT yukang atg5regulatesselectiveautophagyoftheparentalmacronucleusduringitetrahymenaisexualreproduction AT yaliu atg5regulatesselectiveautophagyoftheparentalmacronucleusduringitetrahymenaisexualreproduction AT jingxu atg5regulatesselectiveautophagyoftheparentalmacronucleusduringitetrahymenaisexualreproduction AT weiwang atg5regulatesselectiveautophagyoftheparentalmacronucleusduringitetrahymenaisexualreproduction
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Atg5 Regulates Selective Autophagy of the Parental Macronucleus during <i>Tetrahymena</i> Sexual Reproduction

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