In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes

Abstract In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PT...

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Autores principales:	Mahmood Barani, Mohammad Reza Hajinezhad, Saman Sargazi, Abbas Rahdar, Sheida Shahraki, Azadeh Lohrasbi-Nejad, Francesco Baino
Formato:	article
Lenguaje:	EN
Publicado:	Springer 2021
Materias:	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/14bfcb953f5743f69b1ff21efd0bf119
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spelling	oai:doaj.org-article:14bfcb953f5743f69b1ff21efd0bf1192021-12-05T12:19:14ZIn vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes10.1007/s10856-021-06623-60957-45301573-4838https://doaj.org/article/14bfcb953f5743f69b1ff21efd0bf1192021-12-01T00:00:00Zhttps://doi.org/10.1007/s10856-021-06623-6https://doaj.org/toc/0957-4530https://doaj.org/toc/1573-4838Abstract In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PTX was assessed using MCF7, Hela, and HUVEC cell lines. In order to evaluate the in vivo efficacy of niosomal PTX in rats as compared to free PTX, the animals were intraperitoneally administered with 2.5 mg/kg and 5 mg/kg niosomal PTX for two weeks. Results showed that the pH-responsive niosomes had a nanometric size, spherical morphology, 77% EE, and pH-responsive release in pH 5.2 and 7.4. Compared with free PTX, we found markedly lower IC50s when cancer cells were treated for 48 h with niosomal PTX, which also showed high efficacy against human cancers derived from cervix and breast tumors. Moreover, niosomal PTX induced evident morphological changes in these cell lines. In vivo administration of free PTX at the dose of 2.5 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. The situation was different when niosomal PTX was administered to the rats: the 5 mg/kg dosage of niosomal PTX significantly increased serum biochemical parameters, but the group treated with the 2.5 mg/kg dose of niosomal PTX showed fewer toxic effects than the group treated with free PTX at the same dosage. Overall, our results provide proof of concept for encapsulating PTX in niosomal formulation to enhance its therapeutic efficacy.Mahmood BaraniMohammad Reza HajinezhadSaman SargaziAbbas RahdarSheida ShahrakiAzadeh Lohrasbi-NejadFrancesco BainoSpringerarticleMaterials of engineering and construction. Mechanics of materialsTA401-492Medical technologyR855-855.5ENJournal of Materials Science: Materials in Medicine, Vol 32, Iss 12, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5
spellingShingle	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5 Mahmood Barani Mohammad Reza Hajinezhad Saman Sargazi Abbas Rahdar Sheida Shahraki Azadeh Lohrasbi-Nejad Francesco Baino In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
description	Abstract In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PTX was assessed using MCF7, Hela, and HUVEC cell lines. In order to evaluate the in vivo efficacy of niosomal PTX in rats as compared to free PTX, the animals were intraperitoneally administered with 2.5 mg/kg and 5 mg/kg niosomal PTX for two weeks. Results showed that the pH-responsive niosomes had a nanometric size, spherical morphology, 77% EE, and pH-responsive release in pH 5.2 and 7.4. Compared with free PTX, we found markedly lower IC50s when cancer cells were treated for 48 h with niosomal PTX, which also showed high efficacy against human cancers derived from cervix and breast tumors. Moreover, niosomal PTX induced evident morphological changes in these cell lines. In vivo administration of free PTX at the dose of 2.5 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. The situation was different when niosomal PTX was administered to the rats: the 5 mg/kg dosage of niosomal PTX significantly increased serum biochemical parameters, but the group treated with the 2.5 mg/kg dose of niosomal PTX showed fewer toxic effects than the group treated with free PTX at the same dosage. Overall, our results provide proof of concept for encapsulating PTX in niosomal formulation to enhance its therapeutic efficacy.
format	article
author	Mahmood Barani Mohammad Reza Hajinezhad Saman Sargazi Abbas Rahdar Sheida Shahraki Azadeh Lohrasbi-Nejad Francesco Baino
author_facet	Mahmood Barani Mohammad Reza Hajinezhad Saman Sargazi Abbas Rahdar Sheida Shahraki Azadeh Lohrasbi-Nejad Francesco Baino
author_sort	Mahmood Barani
title	In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
title_short	In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
title_full	In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
title_fullStr	In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
title_full_unstemmed	In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
title_sort	in vitro and in vivo anticancer effect of ph-responsive paclitaxel-loaded niosomes
publisher	Springer
publishDate	2021
url	https://doaj.org/article/14bfcb953f5743f69b1ff21efd0bf119
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In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes

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