Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats

Background: Chronic ethanol consumption presents toxic effects on liver tissue by inducing oxidative stress and inflammation. The antioxidant and anti-inflammatory properties of lemon verbena were established. Objectives: The present study aimed to evaluate the protective effects of the hydroalcoh...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Emran Habibi, Fatemeh Shaki, Fereshteh Talebpour Amiri, Mahboobeh Feyzi Gharehsou, Mehdi Mokhatari
Formato:	article
Lenguaje:	EN
Publicado:	Mazandaran University of Medical Sciences 2021
Materias:	aloysia citriodora palau ethanol lemon verbena liver toxicity oxidative damage inflammation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/14c19b54ded443d68606e30fc64a5635
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:14c19b54ded443d68606e30fc64a5635
record_format	dspace
spelling	oai:doaj.org-article:14c19b54ded443d68606e30fc64a56352021-11-07T16:05:09ZEffects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats2423-4494https://doaj.org/article/14c19b54ded443d68606e30fc64a56352021-02-01T00:00:00Zhttps://pbr.mazums.ac.ir/article-1-377-en.htmlhttps://doaj.org/toc/2423-4494Background: Chronic ethanol consumption presents toxic effects on liver tissue by inducing oxidative stress and inflammation. The antioxidant and anti-inflammatory properties of lemon verbena were established. Objectives: The present study aimed to evaluate the protective effects of the hydroalcoholic extract of Aloysia citriodora (A. citriodora) on ethanol-induced hepatotoxicity in male rats by evaluating inflammatory and oxidative stress factors. Methods: The study animals were randomly divided into 7 groups, (6/group) including control, extract alone (400mg/kg), ethanol 10 mg/kg, vitamin C 500 mg/kg + ethanol 10 mg/kg, and the fifth, sixth and seventh groups respectively received an intraperitoneal injection of 100, 200, and 400 mg/kg of A. citriodora extract plus ethanol once a day for 6 weeks. Oxidative stress parameters, such as glutathione content, lipid peroxidation, protein carbonyl, and reactive oxygen species were measured. Furthermore, inflammation parameters (nitric oxide) and liver damage were evaluated by determining the levels of liver enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and histopathological examinations. Results: In the liver tissue of the ethanol-receiving group, a significant increase (P<0.001) was observed in ALT, AST, and ALP levels and pathological changes, compared to the control group. There was also a significant increase in the levels of oxidative stress and inflammatory factors. Interestingly, A. citriodora extract could inhibit ethanol-induced liver damage by suppressing oxidative stress and inflammation. Conclusion: A. citriodora extract significantly attenuated inflammation and oxidative stress caused by ethanol. Therefore, it can be suggested as a beneficial supplement for treating ethanol-induced hepatotoxicity.Emran HabibiFatemeh Shaki Fereshteh Talebpour AmiriMahboobeh Feyzi GharehsouMehdi MokhatariMazandaran University of Medical Sciencesarticlealoysia citriodora palauethanollemon verbenaliver toxicityoxidative damageinflammationTherapeutics. PharmacologyRM1-950ENPharmaceutical and Biomedical Research, Vol 7, Iss 3, Pp 171-182 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aloysia citriodora palau ethanol lemon verbena liver toxicity oxidative damage inflammation Therapeutics. Pharmacology RM1-950
spellingShingle	aloysia citriodora palau ethanol lemon verbena liver toxicity oxidative damage inflammation Therapeutics. Pharmacology RM1-950 Emran Habibi Fatemeh Shaki Fereshteh Talebpour Amiri Mahboobeh Feyzi Gharehsou Mehdi Mokhatari Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
description	Background: Chronic ethanol consumption presents toxic effects on liver tissue by inducing oxidative stress and inflammation. The antioxidant and anti-inflammatory properties of lemon verbena were established. Objectives: The present study aimed to evaluate the protective effects of the hydroalcoholic extract of Aloysia citriodora (A. citriodora) on ethanol-induced hepatotoxicity in male rats by evaluating inflammatory and oxidative stress factors. Methods: The study animals were randomly divided into 7 groups, (6/group) including control, extract alone (400mg/kg), ethanol 10 mg/kg, vitamin C 500 mg/kg + ethanol 10 mg/kg, and the fifth, sixth and seventh groups respectively received an intraperitoneal injection of 100, 200, and 400 mg/kg of A. citriodora extract plus ethanol once a day for 6 weeks. Oxidative stress parameters, such as glutathione content, lipid peroxidation, protein carbonyl, and reactive oxygen species were measured. Furthermore, inflammation parameters (nitric oxide) and liver damage were evaluated by determining the levels of liver enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and histopathological examinations. Results: In the liver tissue of the ethanol-receiving group, a significant increase (P<0.001) was observed in ALT, AST, and ALP levels and pathological changes, compared to the control group. There was also a significant increase in the levels of oxidative stress and inflammatory factors. Interestingly, A. citriodora extract could inhibit ethanol-induced liver damage by suppressing oxidative stress and inflammation. Conclusion: A. citriodora extract significantly attenuated inflammation and oxidative stress caused by ethanol. Therefore, it can be suggested as a beneficial supplement for treating ethanol-induced hepatotoxicity.
format	article
author	Emran Habibi Fatemeh Shaki Fereshteh Talebpour Amiri Mahboobeh Feyzi Gharehsou Mehdi Mokhatari
author_facet	Emran Habibi Fatemeh Shaki Fereshteh Talebpour Amiri Mahboobeh Feyzi Gharehsou Mehdi Mokhatari
author_sort	Emran Habibi
title	Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
title_short	Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
title_full	Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
title_fullStr	Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
title_full_unstemmed	Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats
title_sort	effects of aloysia citriodora hydroalcoholic extract on ethanol-induced hepatotoxicity in male wistar rats
publisher	Mazandaran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/14c19b54ded443d68606e30fc64a5635
work_keys_str_mv	AT emranhabibi effectsofaloysiacitriodorahydroalcoholicextractonethanolinducedhepatotoxicityinmalewistarrats AT fatemehshaki effectsofaloysiacitriodorahydroalcoholicextractonethanolinducedhepatotoxicityinmalewistarrats AT fereshtehtalebpouramiri effectsofaloysiacitriodorahydroalcoholicextractonethanolinducedhepatotoxicityinmalewistarrats AT mahboobehfeyzigharehsou effectsofaloysiacitriodorahydroalcoholicextractonethanolinducedhepatotoxicityinmalewistarrats AT mehdimokhatari effectsofaloysiacitriodorahydroalcoholicextractonethanolinducedhepatotoxicityinmalewistarrats
_version_	1718443258447659008

Effects of Aloysia citriodora Hydroalcoholic Extract on Ethanol-induced Hepatotoxicity in Male Wistar Rats

Ejemplares similares