Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa

Abstract Protease IV (PIV), a key virulence factor of Pseudomonas aeruginosa is a secreted lysyl-endopeptidase whose expression is induced by quorum sensing (QS). We found that PIV expressed in QS mutant has severe reduction of activity in culture supernatant (CS), even though it is overexpressed to...

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Autores principales:	Jungmin Oh, Xi-Hui Li, Soo-Kyong Kim, Joon-Hee Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14ccc000a8d74b5fbcd9b12f4496a08a
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spelling	oai:doaj.org-article:14ccc000a8d74b5fbcd9b12f4496a08a2021-12-02T11:52:28ZPost-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa10.1038/s41598-017-03733-62045-2322https://doaj.org/article/14ccc000a8d74b5fbcd9b12f4496a08a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03733-6https://doaj.org/toc/2045-2322Abstract Protease IV (PIV), a key virulence factor of Pseudomonas aeruginosa is a secreted lysyl-endopeptidase whose expression is induced by quorum sensing (QS). We found that PIV expressed in QS mutant has severe reduction of activity in culture supernatant (CS), even though it is overexpressed to high level. PIV purified from the QS mutant (M-PIV) had much lower activity than the PIV purified from wild type (P-PIV). We found that the propeptide cleaved from prepro-PIV was co-purified with M-PIV, but never with P-PIV. Since the activity of M-PIV was restored by adding the CS of QS-positive and PIV-deficient strain, we hypothesized that the propeptide binds to and inhibits PIV, and is degraded to activate PIV by a QS-dependent factor. In fact, the CS of the QS-positive and PIV-deficient strain was able to degrade the propeptide. Since the responsible factor should be a QS-dependently expressed extracellular protease, we tested QS-dependent proteases of P. aeruginosa and found that LasB (elastase) can degrade the propeptide and activate M-PIV. We purified the propeptide of PIV and confirmed that the propeptide can bind to and inhibit PIV. We suggest that PIV is post-secretionally activated through the extracellular degradation of the propeptide by LasB, a QS-dependent protease.Jungmin OhXi-Hui LiSoo-Kyong KimJoon-Hee LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungmin Oh Xi-Hui Li Soo-Kyong Kim Joon-Hee Lee Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
description	Abstract Protease IV (PIV), a key virulence factor of Pseudomonas aeruginosa is a secreted lysyl-endopeptidase whose expression is induced by quorum sensing (QS). We found that PIV expressed in QS mutant has severe reduction of activity in culture supernatant (CS), even though it is overexpressed to high level. PIV purified from the QS mutant (M-PIV) had much lower activity than the PIV purified from wild type (P-PIV). We found that the propeptide cleaved from prepro-PIV was co-purified with M-PIV, but never with P-PIV. Since the activity of M-PIV was restored by adding the CS of QS-positive and PIV-deficient strain, we hypothesized that the propeptide binds to and inhibits PIV, and is degraded to activate PIV by a QS-dependent factor. In fact, the CS of the QS-positive and PIV-deficient strain was able to degrade the propeptide. Since the responsible factor should be a QS-dependently expressed extracellular protease, we tested QS-dependent proteases of P. aeruginosa and found that LasB (elastase) can degrade the propeptide and activate M-PIV. We purified the propeptide of PIV and confirmed that the propeptide can bind to and inhibit PIV. We suggest that PIV is post-secretionally activated through the extracellular degradation of the propeptide by LasB, a QS-dependent protease.
format	article
author	Jungmin Oh Xi-Hui Li Soo-Kyong Kim Joon-Hee Lee
author_facet	Jungmin Oh Xi-Hui Li Soo-Kyong Kim Joon-Hee Lee
author_sort	Jungmin Oh
title	Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
title_short	Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
title_full	Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
title_fullStr	Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
title_full_unstemmed	Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa
title_sort	post-secretional activation of protease iv by quorum sensing in pseudomonas aeruginosa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/14ccc000a8d74b5fbcd9b12f4496a08a
work_keys_str_mv	AT jungminoh postsecretionalactivationofproteaseivbyquorumsensinginpseudomonasaeruginosa AT xihuili postsecretionalactivationofproteaseivbyquorumsensinginpseudomonasaeruginosa AT sookyongkim postsecretionalactivationofproteaseivbyquorumsensinginpseudomonasaeruginosa AT joonheelee postsecretionalactivationofproteaseivbyquorumsensinginpseudomonasaeruginosa
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Post-secretional activation of Protease IV by quorum sensing in Pseudomonas aeruginosa

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