Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes

Nadyr A Damasceno,1 Nadia C Miguel,2 Marcelo Palis Ventura,3 Miguel Burnier Jr,4 Marcos P Avila,5 Eduardo F Damasceno3 1Ophthalmology Department, Hospital Naval Marcílio Dias, 2Laboratory of Neurohistology and Cell Ultrastructure, Instituto de Ciências Biomédicas, Un...

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Autores principales:	Damasceno NA, Miguel NC, Ventura MP, Burnier M Jr, Avila MP, Damasceno EF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Sclerotomy Wound Healing Crosslinking Ultraviolet A Riboflavin Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/14cdf3041567438d98184dc109415c9d
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spelling	oai:doaj.org-article:14cdf3041567438d98184dc109415c9d2021-12-02T02:47:25ZScleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes1177-5483https://doaj.org/article/14cdf3041567438d98184dc109415c9d2017-07-01T00:00:00Zhttps://www.dovepress.com/scleral-wound-healing-with-cross-link-technique-using-riboflavin-and-u-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Nadyr A Damasceno,1 Nadia C Miguel,2 Marcelo Palis Ventura,3 Miguel Burnier Jr,4 Marcos P Avila,5 Eduardo F Damasceno3 1Ophthalmology Department, Hospital Naval Marcílio Dias, 2Laboratory of Neurohistology and Cell Ultrastructure, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 3Ophthalmology Department, Universidade Federal Fluminense, Niterói, Brazil; 4Ophthalmology Department, McGill University, Montreal, QC, Canada; 5Ophthalmology Department, Universidade Federal de Goiás, Goiania, Brazil Purpose: The aim of study was to evaluate the cross-link using riboflavin and ultraviolet A (UVA) for improving scleral wound healing.Materials and methods: This was an experimental study involving four New Zealand rabbits (eight eyes). Therapy procedure was chosen for the right eye and control procedure for the left one. UVA irradiation of 365 nm with a surface irradiance of 3 mW/cm2 and a photosensitizer of riboflavin drops were applied for 30 minutes on the right eye at 2 mm from the limbus. Sclerotomy incision was performed at 2 mm from the limbus in both right (on the cross-link-treated area) and left eye. Then, 30 days after surgery, a morphological analysis and histological staining with hematoxylin–eosin and picrosirius red were performed, and the sclerotomy cicatrization of right and left eyes was compared. The variables investigated were as follows: sclerotomy incision pictures and measurements were made using the ImageJ Software. Scleral thickness was measured (employing the anterior optical coherence tomography and the digital caliper). Collagen fiber density stained with picrosirius red staining was measured using the Image Pro Plus software.Results: The morphological analysis showed that in all samples, the right eye presented sclerotomy closure, and in two eyes, among them, there were no visible edges of the sclerotomies incision. The left eye presented sclerotomy closure and incision edges. The Image Pro Plus demonstrated a higher density of collagen fibers in the right eye when compared to the left one. The statistical analysis was significant when compared to the collagen fiber density in the treated eyes with the control eyes.Conclusion: The cross-link procedure resulted in a better sclerotomy wound healing. Keywords: sclerotomy wound healing, cross-linking, ultraviolet A, riboflavinDamasceno NAMiguel NCVentura MPBurnier M JrAvila MPDamasceno EFDove Medical PressarticleSclerotomy Wound HealingCrosslinkingUltraviolet ARiboflavinOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 1265-1272 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sclerotomy Wound Healing Crosslinking Ultraviolet A Riboflavin Ophthalmology RE1-994
spellingShingle	Sclerotomy Wound Healing Crosslinking Ultraviolet A Riboflavin Ophthalmology RE1-994 Damasceno NA Miguel NC Ventura MP Burnier M Jr Avila MP Damasceno EF Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
description	Nadyr A Damasceno,1 Nadia C Miguel,2 Marcelo Palis Ventura,3 Miguel Burnier Jr,4 Marcos P Avila,5 Eduardo F Damasceno3 1Ophthalmology Department, Hospital Naval Marcílio Dias, 2Laboratory of Neurohistology and Cell Ultrastructure, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 3Ophthalmology Department, Universidade Federal Fluminense, Niterói, Brazil; 4Ophthalmology Department, McGill University, Montreal, QC, Canada; 5Ophthalmology Department, Universidade Federal de Goiás, Goiania, Brazil Purpose: The aim of study was to evaluate the cross-link using riboflavin and ultraviolet A (UVA) for improving scleral wound healing.Materials and methods: This was an experimental study involving four New Zealand rabbits (eight eyes). Therapy procedure was chosen for the right eye and control procedure for the left one. UVA irradiation of 365 nm with a surface irradiance of 3 mW/cm2 and a photosensitizer of riboflavin drops were applied for 30 minutes on the right eye at 2 mm from the limbus. Sclerotomy incision was performed at 2 mm from the limbus in both right (on the cross-link-treated area) and left eye. Then, 30 days after surgery, a morphological analysis and histological staining with hematoxylin–eosin and picrosirius red were performed, and the sclerotomy cicatrization of right and left eyes was compared. The variables investigated were as follows: sclerotomy incision pictures and measurements were made using the ImageJ Software. Scleral thickness was measured (employing the anterior optical coherence tomography and the digital caliper). Collagen fiber density stained with picrosirius red staining was measured using the Image Pro Plus software.Results: The morphological analysis showed that in all samples, the right eye presented sclerotomy closure, and in two eyes, among them, there were no visible edges of the sclerotomies incision. The left eye presented sclerotomy closure and incision edges. The Image Pro Plus demonstrated a higher density of collagen fibers in the right eye when compared to the left one. The statistical analysis was significant when compared to the collagen fiber density in the treated eyes with the control eyes.Conclusion: The cross-link procedure resulted in a better sclerotomy wound healing. Keywords: sclerotomy wound healing, cross-linking, ultraviolet A, riboflavin
format	article
author	Damasceno NA Miguel NC Ventura MP Burnier M Jr Avila MP Damasceno EF
author_facet	Damasceno NA Miguel NC Ventura MP Burnier M Jr Avila MP Damasceno EF
author_sort	Damasceno NA
title	Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
title_short	Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
title_full	Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
title_fullStr	Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
title_full_unstemmed	Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes
title_sort	scleral wound healing with cross-link technique using riboflavin and ultraviolet a on rabbit eyes
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/14cdf3041567438d98184dc109415c9d
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Scleral wound healing with cross-link technique using riboflavin and ultraviolet A on rabbit eyes

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