Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting

Abstract Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast...

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Autores principales:	Snehasish Ghosh, Sanchita Mukherjee, Chiranjit Dutta, Kasturee Chakraborty, Paramita Gayen, Somnath Jan, Dhananjay Bhattacharyya, Rituparna Sinha Roy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14d09943b727411a9021042d9db8b705
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spelling	oai:doaj.org-article:14d09943b727411a9021042d9db8b7052021-12-02T16:06:06ZEngineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting10.1038/s41598-017-06360-32045-2322https://doaj.org/article/14d09943b727411a9021042d9db8b7052017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06360-3https://doaj.org/toc/2045-2322Abstract Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibrin (~56 seconds). Our engineered sealants are stabilized by lysine-aspartate ionic interactions and also by Nε(γ-glutamyl) lysine isopeptide bond mediated covalent interaction. Each sealant is formed by two peptides having complementary charges to promote lysine-aspartate ionic interactions and designed isopeptide bond mediated interactions. Computational analysis reveals the isopeptide bond mediated energetically favourable peptide assemblies in sealants 1–3. Our designed sealants 2 and 3 mimic fibrin-mediated clot formation mechanism in presence of transglutaminase enzyme and blood corpuscles. These fibrin-inspired peptides assemble to form sealants having superior hemostatic activities than fibrin. Designed sealants feature mechanical properties, biocompatibility, biodegradability and high adhesive strength. Such nature-inspired robust sealants might be potentially translated into clinics for facilitating efficient blood clotting to handle traumatic coagulopathy and impaired blood clotting.Snehasish GhoshSanchita MukherjeeChiranjit DuttaKasturee ChakrabortyParamita GayenSomnath JanDhananjay BhattacharyyaRituparna Sinha RoyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Snehasish Ghosh Sanchita Mukherjee Chiranjit Dutta Kasturee Chakraborty Paramita Gayen Somnath Jan Dhananjay Bhattacharyya Rituparna Sinha Roy Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
description	Abstract Designing biologically inspired nanoscale molecular assembly with desired functionality is a challenging endeavour. Here we report the designing of fibrin-inspired nanostructured peptide based sealants which facilitate remarkably fast entrapping of blood corpuscles (~28 seconds) in contrast to fibrin (~56 seconds). Our engineered sealants are stabilized by lysine-aspartate ionic interactions and also by Nε(γ-glutamyl) lysine isopeptide bond mediated covalent interaction. Each sealant is formed by two peptides having complementary charges to promote lysine-aspartate ionic interactions and designed isopeptide bond mediated interactions. Computational analysis reveals the isopeptide bond mediated energetically favourable peptide assemblies in sealants 1–3. Our designed sealants 2 and 3 mimic fibrin-mediated clot formation mechanism in presence of transglutaminase enzyme and blood corpuscles. These fibrin-inspired peptides assemble to form sealants having superior hemostatic activities than fibrin. Designed sealants feature mechanical properties, biocompatibility, biodegradability and high adhesive strength. Such nature-inspired robust sealants might be potentially translated into clinics for facilitating efficient blood clotting to handle traumatic coagulopathy and impaired blood clotting.
format	article
author	Snehasish Ghosh Sanchita Mukherjee Chiranjit Dutta Kasturee Chakraborty Paramita Gayen Somnath Jan Dhananjay Bhattacharyya Rituparna Sinha Roy
author_facet	Snehasish Ghosh Sanchita Mukherjee Chiranjit Dutta Kasturee Chakraborty Paramita Gayen Somnath Jan Dhananjay Bhattacharyya Rituparna Sinha Roy
author_sort	Snehasish Ghosh
title	Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_short	Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_full	Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_fullStr	Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_full_unstemmed	Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
title_sort	engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/14d09943b727411a9021042d9db8b705
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Engineered isopeptide bond stabilized fibrin inspired nanoscale peptide based sealants for efficient blood clotting

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